Synthesis, characterization, and application of a novel multifunctional stationary phase for hydrophilic interaction/reversed phase mixed-mode chromatography

Aral, Hayriye; Çelik, Kadir Serdar; Altındağ, Ramazan; Aral, Tarık

doi:10.1016/j.talanta.2017.07.014

Cited by 35 publications

(19 citation statements)

References 47 publications

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“…Elemental analysis of the synthesized PFB‐SP showed that the carbon, hydrogen, and nitrogen contents were 5.9, 0.80, and 1.20%, respectively, Supporting Information Table S1. The results between the three batches were close, indicating repeatability . The results from SEM images (Supporting Information Figure S1) indicated that the SP did not show modification in morphology of the silica particles and that no agglomerates were formed that may influence the packing process.…”

Section: Resultsmentioning

confidence: 69%

Synthesis and evaluation of a pentafluorobenzamide stationary phase for HPLC separations in the reversed phase and hydrophilic interaction modes

Ferreira

Gama

Silva

et al. 2018

J of Separation Science

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A pentafluorobenzamide stationary phase was synthesized by an easy method with no intermediate purification steps. Physicochemical characterization (elemental analysis, fourier transform infrared spectroscopy, Si and C nuclear magnetic resonance spectroscopy) confirmed the presence of pentafluorobenzamide functionalization on the surface of the silica particles. The pentafluorobenzamide stationary phase proved to be quite versatile as it can be used in two different modes in liquid chromatography: reversed phase and hydrophilic interaction liquid chromatography. Chromatographic characterizations in both modes confirmed the multiple interactions established by the new stationary phase, such as hydrogen bonding and π-π and ion-exchange interactions. The pentafluorobenzamide stationary phase was successfully employed for the separation of nucleosides and antihypertensive drugs under hydrophilic interaction liquid chromatography conditions, as well as pesticides and benzodiazepine using reversed phase conditions. The stationary phase showed significant potential when compared with commercial columns.

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Section: Resultsmentioning

confidence: 69%

Synthesis and evaluation of a pentafluorobenzamide stationary phase for HPLC separations in the reversed phase and hydrophilic interaction modes

Ferreira

Gama

Silva

et al. 2018

J of Separation Science

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“…The π–π interactions might have also affected the separation of the phenols. The strong π‐basic or π‐acidic rings interacted strongly with the stationary benzene rings [37]; thus, the 2,3‐dichlorophenol containing two electron‐withdrawing chloro‐ groups contained a strong π‐acidic aromatic ring, which increased the retention time. Furthermore, the resolutions and retentions of the studied analytes increased gradually with the decreasing ACN contents in the mobile phase.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of microporous organic network@silica composite for high‐performance liquid chromatographic separation of drugs and proteins

2021

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Microporous organic networks (MONs) that exhibit good stability and hydrophobicity are promising candidates for performing HPLC separation of small organic compounds. However, their applications in separating large analytes as well as biomolecules are still limited by the microporous nature of MONs. Herein, we demonstrated the fabrication of a MON-functionalized silica (MON@SiO 2 ), exhibiting micro and mesopores for the HPLC separations of small drugs as well as large analytes, such as flavones, nonsteroidal anti-inflammatory drugs (NSAIDs), endocrine disrupting chemicals (EDCs), and proteins. MON was successfully modified on SiO 2 microspheres to yield the uniform and mono-dispersed MON@SiO 2 . The separation mechanisms and performance of the MON@SiO 2 packed column were evaluated for a wide range of analytes, including neutral, acidic, basic compounds, drugs, and proteins. Compared with commercial C18 and SiO 2 -NH 2 packed columns, the proposed MON@SiO 2 column afforded superior performance in the separations of flavones, NSAIDs, EDCs, and proteins. Moreover, the MON@SiO 2 column also offered good repeatability with intraday RSDs (n = 7) of <0.1%, <2.0%, <2.3%, and <0.7% for the retention time, peak height, peak area, and half peak width, respectively, for separating EDCs. This work proved the potential of using MONs in the HPLC separations of drugs and proteins.

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“…A multifunctional phase, synthesized from L-isoleucine and 4-phenylbutylamine, was used as a mixed-mode HILIC/RPLC phase. 98 This new stationary phase was found to be particularly…”

Section: Hilic For Polar and Ionizable Compoundsmentioning

confidence: 92%

Recent Advances in Chromatography for Pharmaceutical Analysis

et al. 2018

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Synthesis, characterization, and application of a novel multifunctional stationary phase for hydrophilic interaction/reversed phase mixed-mode chromatography

Cited by 35 publications

References 47 publications

Synthesis and evaluation of a pentafluorobenzamide stationary phase for HPLC separations in the reversed phase and hydrophilic interaction modes

Synthesis and evaluation of a pentafluorobenzamide stationary phase for HPLC separations in the reversed phase and hydrophilic interaction modes

Fabrication of microporous organic network@silica composite for high‐performance liquid chromatographic separation of drugs and proteins

Recent Advances in Chromatography for Pharmaceutical Analysis

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