Synthesis, characterization and biocompatibility of chitosan functionalized superparamagnetic nanoparticles for heat activated curing of cancer cells

Abstract

Surface functionalization, colloidal stability and biocompatibility of magnetic nanoparticles are crucial for their biological applications. Here, we report a synthetic approach for the direct preparation of superparamagnetic nanoparticles consisting of a perovskite LSMO core modified with a covalently linked chitosan shell that provides colloidal stability in aqueous solutions for cancer hyperthermia therapy. The characterization of the core-shell nanostructure using Fourier transform infrared spectroscopy; thermo-gravimetric analysis to assess the chemical bonding of chitosan to nanoparticles; field-emission scanning electron microscopy and transmission electron microscopy for its size and coating efficiency estimation; and magnetic measurement for their magnetization properties was performed. Zeta potential and light scattering studies of the core shell revealed it to possess good colloidal stability. Confocal microscopy and MTT assay are performed for qualitative and quantitative measurement of cell viability and biocompatibility. In depth cell morphology and biocompatibility is evaluated by using multiple-staining of different dyes. The magnetic@chitosan nanostructure system is found to be biocompatible up to 48 h with 80% cell viability. Finally, an in vitro cancer hyperthermia study is done on the MCF7 cell line. During in vitro hyperthermia treatment of cancer cells, cell viability is reduced upto 40% within 120 min with chitosan coated nanoparticles. Our results demonstrate that this simplified and facile synthesis strategy shows potential for designing a colloidal stable state and biocompatible core shell nanostructures for cancer hyperthermia therapy.

Hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3 nanoparticles: in vivo studies

“…Despite several in vitro reports demonstrating the potential ability of LSMO nanoparticles in cancer treatment, [18][19][20] studies on in vivo tumor regression have not yet been attempted. The present study seeks to fill this knowledge gap by evaluating RF-induced Dex-LSMO nanoparticlesmediated hyperthermia to be used for tumor regression in melanoma-bearing C57BL/6J mice.…”

Safety and toxicology of the intravenous administration of Ang2-siRNA plasmid chitosan magnetic nanoparticles

“…The surface functionalization, colloidal stability and biocompatibility of the magnetic nanoparticles are crucial for their biomedical applications (22), and the toxicity of the nanoparticles is often closely associated with their sizes. When the particle diameter is <100 nm, the toxicity is predominantly decided by the nature of the particles or the surface-attached materials, but when the particle size is larger, death can often be caused by the embolism of capillaries and small blood vessels by the particles.…”

Multicomponent, Tumor-Homing Chitosan Nanoparticles for Cancer Imaging and Therapy

“…MR contrast agents using chitosan nanoparticles have been reported for cancer MR imaging [29,30,31,32,33,34,35,36]. Nwe et al reported T 1 contrast agents using 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid 1-(2,5-dioxo-1-pyrrolidinyl) ester (DOTA-NHS) and water-soluble glycol chitosan (GC) polymer, followed by Gd chelation [31].…”