Synthesis, characterization, and cytotoxicity of glutathione-PEG-iron oxide magnetic nanoparticles

Haddad, Paula S.; Santos, Marconi C.; Cassago, Carolina Aparecida de Guzzi; Bernardes, Juliana S.; Jesus, Marcelo Bispo de; Seabra, Amedea B.

doi:10.1007/s11051-016-3680-y

Cited by 20 publications

(11 citation statements)

References 70 publications

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“…Higher hydrodynamic sizes of NPs measured by DLS, in comparison with the sizes obtained by AFM or XRD, are attributed to the presence of extra hydrate layers in aqueous environments. 10,18,19 The zeta potential was -22.10 AE 0.55 mV. These results indicate that thiolated nanoparticles have a hydrodynamic diameter in the nanoscale and a moderate polydispersity index, indicating a good dispersion in water, which makes these NPs attractive vehicles for drug delivery in biomedical applications.…”

Section: Synthesis and Characterization Of Fe 3 O 4 -Msa Npsmentioning

confidence: 76%

“…Our group has been synthesizing Fe 3 O 4 NPs by coprecipitation and thermal decomposition methods, followed by NP surface functionalization with different coatings that can act as carriers of nitric oxide (NO), including thiol molecules such as mercaptosuccinic acid (MSA), dimercaptosuccinic acid (DMSA), [11][12][13][14] glutathione (GSH), cysteine (Cys), 8,9,15 silica, 16,17 and polyethylene glycol (PEG). 18,19 Nitric oxide (NO) is an important biological molecule involved in several physiological processes such as the promotion of wound healing, the control of blood ow, and antimicrobial and anticancer activities. [20][21][22][23][24] However, as a free radical, the potential therapeutic applications of NO have been limited by its instability and short half-life.…”

Section: Introductionmentioning

confidence: 99%

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Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications

et al. 2017

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Section: Synthesis and Characterization Of Fe 3 O 4 -Msa Npsmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications

et al. 2017

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“…Total levels of NO were quantified by measuring nitrite (NO 2 − ), nitrate (NO 3 − ), and S-nitrosothiol contents by using the WPI TBR4100/1025 amperometric NO sensor (World Precision Instruments Inc., Sarasota, FL, USA) with the nitric oxide specific ISO-NOP sensor (2 mm) [ 30 , 31 ]. For quantification of S-nitrosothiols, a solution of CuCl 2 (100 mmol L −1 ) was used, and for NO 2 − , a solution of potassium iodide (KI, 100 mmol·L −1 ) in sulfuric acid (H2SO 4 , (100 mmol·L −1 ) was used.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial Dysfunction in Cardiorenal Syndrome 3: Renocardiac Effect of Vitamin C

Neres-Santos

Junho

Panico

et al. 2021

Cells

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Cardiorenal syndrome (CRS) is a pathological link between the kidneys and heart, in which an insult in a kidney or heart leads the other organ to incur damage. CRS is classified into five subtypes, and type 3 (CRS3) is characterized by acute kidney injury as a precursor to subsequent cardiovascular changes. Mitochondrial dysfunction and oxidative and nitrosative stress have been reported in the pathophysiology of CRS3. It is known that vitamin C, an antioxidant, has proven protective capacity for cardiac, renal, and vascular endothelial tissues. Therefore, the present study aimed to assess whether vitamin C provides protection to heart and the kidneys in an in vivo CRS3 model. The unilateral renal ischemia and reperfusion (IR) protocol was performed for 60 min in the left kidney of adult mice, with and without vitamin C treatment, immediately after IR or 15 days after IR. Kidneys and hearts were subsequently collected, and the following analyses were conducted: renal morphometric evaluation, serum urea and creatinine levels, high-resolution respirometry, amperometry technique for NO measurement, gene expression of mitochondrial dynamic markers, and NOS. The analyses showed that the left kidney weight was reduced, urea and creatinine levels were increased, mitochondrial oxygen consumption was reduced, NO levels were elevated, and Mfn2 expression was reduced after 15 days of IR compared to the sham group. Oxygen consumption and NO levels in the heart were also reduced. The treatment with vitamin C preserved the left kidney weight, restored renal function, reduced NO levels, decreased iNOS expression, elevated constitutive NOS isoforms, and improved oxygen consumption. In the heart, oxygen consumption and NO levels were improved after vitamin C treatment, whereas the three NOS isoforms were overexpressed. These data indicate that vitamin C provides protection to the kidneys and some beneficial effects to the heart after IR, indicating it may be a preventive approach against cardiorenal insults.

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“…Two generic types of modification have been used in the past. One is that hydrophobic nanoparticles are coated with a hydrophilic polymer, for example, dextran, 22 polyethylene glycol (PEG), 21,23,24 chitosan 25 and so on, [26][27][28][29] the other is that hydrophobic nanoparticles are encapsulated in a hydrophilic polymer and form a new carrier, 30 which are somewhat larger than iron oxide nanoparticles, and have a higher relaxivity per individual particle. However, as the particle size increases, it will be difficult to cross the blood-brain barrier (BBB).…”

Section: Introductionmentioning

confidence: 99%

Citric acid coated ultrasmall superparamagnetic iron oxide nanoparticles conjugated with lactoferrin for targeted negative MR imaging of glioma

et al. 2020

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The proposed study was to develop the preparation of ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) modified with citric acid, with surface conjugated with lactoferrin (Lf), which used as a potential targeted contrast agent for magnetic resonance imaging (MRI) of brain glioma. USPIONs were prepared by the thermal decomposition method. The hydrophobic USPIONs were coated with citric acid by the ligand exchange method. Then, Lf was conjugated into the surface of USPIONs. The obtained Lf-USPIONs were analyzed by fourier transform infrared (FTIR) spectroscopy and polyacrylamide gel electrophoresis. The size, size distribution, shape and superparamagnetic property of Lf-USPIONs were investigated with TEM and vibrating sample magnetometer (VSM). Both FTIR and electrophoresis analysis demonstrated the successful conjugation of Lf to the surface of USPIONs. The average size of Lf-USPIONs was about 8.4 ± 0.5 nm, which was determined using the statistics of measured over 100 nanoparticles in the TEM image, with a negative charge of −7.3 ± 0.2 mV. TEM imaging revealed that Lf-USPIONs were good in dispersion and polygonal in morphology. VSM results indicated that Lf-USPIONs were superparamagnetic and the saturated magnetic intensity was about 69.8 emu/g. The Lf-USPIONs also showed good biocompatibility in hemolysis, cytotoxicity, cell migration and blood biochemistry studies. MR imaging results in vitro and in vivo indicated that Lf-USPIONs exhibited good negative contrast enhancement. Taken together, Lf-USPIONs hold great potential for brain gliomas MR imaging as a nanosized targeted contrast agent.

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Synthesis, characterization, and cytotoxicity of glutathione-PEG-iron oxide magnetic nanoparticles

Cited by 20 publications

References 70 publications

Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications

Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications

Mitochondrial Dysfunction in Cardiorenal Syndrome 3: Renocardiac Effect of Vitamin C

Citric acid coated ultrasmall superparamagnetic iron oxide nanoparticles conjugated with lactoferrin for targeted negative MR imaging of glioma

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