Synthesis, characterization and cytotoxicity of novel modified poly[(maleic anhydride)‐ co‐(vinyl acetate)]/noradrenaline conjugate

Abstract

Poly[(maleic anhydride)‐co‐(vinyl acetate)] (MAVA) copolymer was synthesized by free radical polymerization reaction, in methyl ethyl ketone at 80 °C, using benzoyl peroxide as the initiator. The copolymer was then modified with a biomolecule, noradrenaline (NA). The modification reaction was performed at 70 °C in dimethylformamide containing triethylamine as the catalyst. The modified polymer was named MAVA/NA. Structural characterization of the copolymer and the modified product was carried out by Fourier transform infrared (FTIR) and 1H NMR and 13C NMR spectroscopy. The FTIR, 1H NMR and 13C NMR spectra confirmed that NA was successfully covalently bound to the MAVA copolymer backbone. Surface morphology was visualized by atomic force microscopy. The cumulative release of NA from MAVA/NA was determined in phosphate buffered saline solution for 7 days at 37 °C and compared with MAVA. Cytotoxicity of the MAVA/NA was evaluated by using a mouse fibroblast cell line (L929). Results obtained indicated that MAVA/NA had almost no toxicity and no negative effect on cell viability at 250 µg mL−1 concentration. © 2012 Society of Chemical Industry

A New Family of Photoluminescent Polymers with Dual Chromophores

“…The chemical shift at δ = 218 ppm can be attributed to C(13) . The characteristic peaks appearing at δ = 181, 178, and 176 ppm belonged to C(7), C(8), and C(5), respectively . The broad peaks at δ = 73, 55, 44, and 35 ppm pertained to C(4), C(1), C(2), and C(3), respectively, in the backbone, whereas the sharp peaks at δ = 73, 57, 35, 32, and 23 ppm can be attributed to C(9), C(12), C(14), C(10,11), and C(6), respectively.…”

“…The other peaks at 1375, 1245, and 1028 cm −1 can be ascribed to CH 3 , COC, and CO, respectively. [11] The inverse-gated decoupling 13 C NMR spectrum of PMV/Na is shown in Figure S2b (Supporting Information). The chemical shift at δ = 218 ppm can be attributed to C (13).…”

“…[11] The inverse-gated decoupling 13 C NMR spectrum of PMV/Na is shown in Figure S2b (Supporting Information). The chemical shift at δ = 218 ppm can be attributed to C (13). [12] The characteristic peaks appearing at δ = 181, 178, and 176 ppm belonged to C(7), C(8), and C(5), respectively.…”

“…[12] The characteristic peaks appearing at δ = 181, 178, and 176 ppm belonged to C(7), C(8), and C(5), respectively. [13] The broad peaks at δ = 73, 55, 44, and 35 ppm pertained to C(4), C(1), C(2), and C(3), respectively, in the backbone, whereas the sharp peaks at δ = 73, 57, 35, 32, and 23 ppm can be attributed to C(9), C(12), C(14), C(10,11), and C(6), respectively. Based on the above information pertaining to FTIR and NMR, it is reasonable to accept the molecular structure of PMV/Na shown in Figure 2a.…”

Zwitterionic amphiphile coated magnetofluorescent nanoparticles – synthesis, characterization and tumor cell targeting

“…The spectrum comparison and analysis indicates the formation of SB after the reaction of the tertiary amines of PMAO-DMAPA with 1,3-propanesultone. 43–46 Peaks associated with CB in PMAO-CB-SB are not significant for observation, probably because the peaks for the additional carboxyl C=O from the formed CB groups in PMAO-CB-SB are overlapping with those of the existing carboxyl C=O in PMAO-DMAPA. However, a slight distortion at around 1590 cm −1 in the spectrum of PMAO-CB-SB (not marked but close to the mark line at 1560 cm −1 ) is still distinguishable.…”