Synthesis, characterization and DNA-binding characteristics of Ru(II) molecular light switch complexes

Devi, Ch. Sarala; Nagababu, Penumaka; Shilpa, M.; Kumar, Yata Praveen; Reddy, M. Rajender; Gabra, Nazar Md; Satyanarayana, S.

doi:10.1007/s13738-012-0150-z

Cited by 9 publications

(5 citation statements)

References 39 publications

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“…Light switch studies were conducted using a literature method . As shown in Figure , upon addition of DNA to complexes 1 , 2 and 3 , its emission intensity is increased (light switch on).…”

Section: Resultsmentioning

confidence: 99%

“…Many cobalt(II) complexes can cleave DNA under irradiation by UV or visible light. When circular plasmid DNA is subjected to electrophoresis, relatively fast migration will be observed in the intact supercoiled form (Form I); if scission occurs on one strand (nicked), the supercoiled form will relax to generate a slower‐moving open circular form (Form II) . Figure shows the extent of DNA photo‐cleavage with 50 μM concentration of complexes 1 , 2 and 3 at 365 nm for 60 min.…”

Section: Resultsmentioning

confidence: 99%

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Cobalt(II)–metformin complexes containing α‐diimine/α‐diamine as auxiliary ligand: DNA binding properties

Vasantha

Kumar

Shekhar

et al. 2017

Applied Organom Chemis

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The cobalt(II) complexes [Co(Cl)2(met)(o‐phen)] (1), [Co(Cl)2(en)(met)] (2) and [Co(Cl)2(met)(opda)] (3) (met = metformin, o‐phen = ortho‐phenanthroline, en = ethylenediamine, opda = ortho‐phenylenediamine) were synthesized and characterized using liquid chromatography–mass spectrometry, elemental analysis, molar conductance measurements, thermal analysis, infrared spectroscopy, magnetic moment measurements, electronic spectroscopy and X‐ray diffraction. The metal centre was found to be in an octahedral geometry. UV–visible absorption, fluorescence and viscosity measurements were conducted to assess the interaction of the complexes with calf thymus DNA. The complexes showed absorption hyperchromism in UV–visible spectra with DNA. The binding constants from UV–visible absorption studies were 1.38 × 105, 2.1 × 105 and 3.1 × 105 M−1 for 1, 2 and 3, respectively, and Stern–Volmer quenching constants from fluorescence studies were 0.146, 0.176 and 0.475, respectively. Viscosity measurements revealed that the binding of the complexes with DNA could be surface binding, mainly due to groove binding. The activities of the complexes in DNA cleavage decrease in the order 3 > 2 > 1. The complexes were docked into DNA topoisomerase II using Discovery Studio 2.1 software.

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“…Light switch studies were conducted using a literature method . As shown in Figure , upon addition of DNA to complexes 1 , 2 and 3 , its emission intensity is increased (light switch on).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Cobalt(II)–metformin complexes containing α‐diimine/α‐diamine as auxiliary ligand: DNA binding properties

Vasantha

Kumar

Shekhar

et al. 2017

Applied Organom Chemis

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“…The hypochromism (H%) of the metal-to-ligand charge transfer (MLCT) bands in complexes 1 and 2, defined as H% ¼ 100 Â (A free ÀA bound )/A free , where A ¼ absorption, was determined to be about 8.7 AE 0.05 and 7.5 AE 0.05 %, respectively. In order to compare the DNA-binding affinities of the two complexes quantitatively, their intrinsic binding constants (phenanthroline)), [18] since the intercalator for complexes 1 and 2 is (4HEPIP), which has additional groups such as hydroxyl and ethoxy which may exert some steric hindrance. These steric clashes then prevent the complexes from intercalating effectively and thus cause a decrease of the intrinsic binding constants.…”

Section: Fluorescence Studiesmentioning

confidence: 99%

“…[12][13][14] However, there are only a few reports on ruthenium(II) complexes with monodentate ancillary ligands. [15][16][17][18] Chen et al reported that many Ru II polypyridyl complexes are less soluble in water due to their big hydrophobic ligands. [19] This limits their applications as good probes of biological systems or anticancer drugs.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis, Characterisation, Interaction with DNA, Cytotoxicity, and Apoptotic Studies of Ruthenium(II) Polypyridyl Complexes

et al. 2014

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We report the synthesis and characterisation of two new ruthenium(II) polypyridyl complexes containing monodentate ancillary ligands [Ru(L) 4 (4HEPIP)], where L ¼ 4-aminopyridine (1) or pyridine (2) and 4HEPIP ¼ 2-(4-hydroxy-3-ethoxyphenyl)-1H-imidazo[4,5-f][1,10](phenanthroline). These complexes were characterised by elemental analysis and ultraviolet-visible, infrared, and 1 H NMR spectroscopy. The binding properties of the two complexes towards calf thymus (CT)-DNA were investigated with different spectrophotometric methods, viscosity measurements, and salt dependent studies. Experimental results indicated that the complexes interact with CT-DNA base pairs by intercalation. Upon irradiation at 365 nm, these complexes efficiently cleave pBR322 DNA from super coiled form I to nicked form II. Their cytotoxicity on different cancer cell lines such as A549, Du145, and HeLa was investigated. The IC 50 values are 39.5, 28.3, and 27.3 mM for complex 1, and 55, 67.9, and 47.9 mM for complex 2 respectively. Cellular uptake and apoptosis induced by these complexes was also studied.

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DNA-binding and cleavage, cytotoxicity properties of Ru(II) complexes with 2-(4′-chloro-phenyl) imidazo[4,5-f][1,10]phenanthroline, ligand and their “light switch” on–off effect

et al. 2013

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Synthesis, characterization and DNA-binding characteristics of Ru(II) molecular light switch complexes

Cited by 9 publications

References 39 publications

Cobalt(II)–metformin complexes containing α‐diimine/α‐diamine as auxiliary ligand: DNA binding properties

Cobalt(II)–metformin complexes containing α‐diimine/α‐diamine as auxiliary ligand: DNA binding properties

Synthesis, Characterisation, Interaction with DNA, Cytotoxicity, and Apoptotic Studies of Ruthenium(II) Polypyridyl Complexes

DNA-binding and cleavage, cytotoxicity properties of Ru(II) complexes with 2-(4′-chloro-phenyl) imidazo[4,5-f][1,10]phenanthroline, ligand and their “light switch” on–off effect

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