Synthesis, characterization, antimicrobial and antimetastatic activity of silver nanoparticles synthesized from Ficus ingens leaf

Abstract

Cancer incidence is still increasing due to inadequate responsive treatments. Inertness and biocompatibility of nanoparticles synthesized using plant extracts have shown therapeutic applications and make it to be a good anti-cancer candidates. This study is a recent novel spotlight that synthesized silver nanoparticle from Ficus ingens leaf extract (FILE) and studied its anti-metastatic and anti-bacterial activity. The chemical and surface analysis of the synthesized FILE silver nanoparticles (FILE-AgNPs) was studied using UV-visible, Fourier transform infrared (FTIR), X-ray diffraction (XRD), zeta sizer, atomic force microscopy (AFM) and scanning electron microscopy (SEM). Gas chromatography mass spectrometric (GC-MS) and quantitative photochemical analyses were also carried out. The antimicrobial activity of FILE-AgNPs was found to be effective with MIC of 10 mg/mL for E. coli and 20 mg/mL for S. typhi and B. cereus with significance difference. Toxicity, proliferation and antimetastatic potential of FILE-AgNPs were studied on MDA-MB 231 cell models using tryphan blue, MTT and wound heal assay, respectively. FILE-AgNPs showed the ability to inhibit metastasis of MDA-MB 231 cells in dose-dependent manner in which 10 lg/mL and 5 lg/mL inhibit by 96% and 75%, respectively. The synthesized FILE-AgNPs are remarkable candidates for treatment of cancer cases and other cancer related cases.

Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: a review of recent literature

“… 109 The MIC of 50 μg mL −1 was recorded for S. cereus whereas, it was 100 μg mL −1 for E. coli . This finding is in sharp contrast to the work of Kavaz et al 107 mentioned earlier where Gram-negative bacteria has lower MIC than Gram-positive bacteria. However, Erci et al 108 defended their finding of the more pronounced effect of AgNPs against Gram-positive bacteria than Gram-negative bacteria based on the structural difference in cell wall composition of Gram-positive and Gram-negative bacteria.…”

“…However, the nanoparticle formation takes place suddenly after 60 s of microwave irradiation. Ficus ingens mediated AgNPs recorded MIC value of 10 μg mL −1 on E. coli and 20 μg mL −1 on both S. typhi and B. cereus 107 which is in close agreement with the earlier report of 10 μg mL −1 for E. coli . The AgNPs showed highest inhibition against E. coli and least with S. cereus .…”

Phytofabrication of Silver Nanoparticles (AgNPs) with Pharmaceutical Capabilities Using Otostegia persica (Burm.) Boiss. Leaf Extract

“…In the FTIR spectrum in the range from 500 to 4000 cm −1 of such phytofabricated AgNPs, the following absorption peaks were observed: 3421, 2923, 1755, 1661, 1524, 1376, 1254, 1048, and 697 cm −1 . These absorption peaks were attributed to the O-H stretching vibrations (3421 cm −1 ) in alcohols, phenols, and flavonoids compounds [28], the C-H stretching vibrations (2923 cm −1 ) of aromatic compounds [27], the C=O stretching vibrations (1755 cm −1 ) for carbonyl compounds [29], the N-H stretching vibrations (1661 cm −1 ) of amide (I) in proteins [30], the C=C stretching vibrations (1524 cm −1 ) of aromatic compounds [31], the C-H stretching vibrations (1376 cm −1 ) in methylene moieties [32], the C-N stretching vibrations (1254 cm −1 ) of aliphatic amines [33], the C-O stretching vibrations (1048 cm −1 ) in alcohols and phenols [34], and finally, the C-H bonding vibrations (697 cm −1 ) of aromatic compounds [35]. In the FTIR spectrum of the O. persica leaf extract, the corresponding absorption peaks were observed at 3417, 2917, 2843, 1742, 1653, 1510, 1442, 1375, 1246, 1044, and 694 cm −1 , and as before related to the appropriate vibrations of the O-H, C-H, C-H, C=O, N-H, C=C, -OH, C-H, C-N, C-O, and C-H groups, respectively.…”

Synthesis of Silver Nanoparticles from the Polysaccharide of Farfarae Flos and Uncovering Its Anticancer Mechanism Based on the Cell Metabolomic Approach

“…The size of FFP@AgNPs was 69.79 nm (Figure A). The PDI (polydispersity index) of FFP@AgNPs was found to be 0.217, suggesting that the synthesized nanoparticles were monodispersed . Moreover, TEM analysis was further used to reveal the morphology, shape, and size of FFP@AgNPs (Figure B).…”