Synthesis, DNA binding, hemolytic, and anti-cancer assays of curcumin I-based ligands and their ruthenium(III) complexes

Ali, Imran; Saleem, Kishwar; Wesselinova, Diana; Haque, Ahasanul

doi:10.1007/s00044-012-0133-8

Cited by 118 publications

(36 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies already revealed a selective uptake of glucose‐substituted ruthenium complexes in HepG2 cells, which could contribute to the enhanced cytotoxic effects seen in our investigations . Furthermore, increased cytotoxicity of curcumin as well as curcumin‐conjugated metal complexes against HepG2 cells is consistent with previous reports . Correlation between cytotoxicity and specific uptake of d ‐fructose‐conjugated compounds requires further investigations.…”

Section: Resultssupporting

confidence: 92%

Synthesis and in vitro Toxicity of d‐Glucose and d‐Fructose Conjugated Curcumin–Ruthenium Complexes

et al. 2016

View full text Add to dashboard Cite

A series of carbohydrate‐conjugated bis(demethoxy)curcumin (BDC) ligands were synthesized by using the Huisgen copper(I)‐catalyzed cycloaddition between azido‐functionalized d‐glucose and d‐fructose as well as propargyl‐modified BDC. The unprotected sugar ligands were treated with Ru(bpy)2Cl2 to form curcumin‐conjugated Ru complexes of general formula Ru(bpy)2(L)Cl. The ligands as well as Ru complexes were analyzed by NMR, IR, UV/Vis, and fluorescence spectroscopy, mass spectrometry as well as by elemental analysis (EA). Incubation of L929, HepG2 and the breast cancer cell line MDA‐MB‐231 revealed lower cytotoxicity of all carbohydrate‐conjugated ligands compared with BDC. The Ru complexes exhibited higher cytotoxicity than the parent ligands, in particular against HepG2 cells, whereas the noncancerous L929 cell line remained unaffected. Unexpectedly, the d‐fructose‐conjugated ligand and its corresponding Ru complex did not show any significant toxicity against MDA‐MB‐231 cells.

show abstract

Section: Resultssupporting

confidence: 92%

Synthesis and in vitro Toxicity of d‐Glucose and d‐Fructose Conjugated Curcumin–Ruthenium Complexes

et al. 2016

View full text Add to dashboard Cite

show abstract

“…This frequently results in dreadful side effects in patients including a drastic drop in cell counts (WBC and RBC) and severe anemic conditions. Although erythrocytes are not good models for normal cells, to understand whether asparaginase is hemocompatible, hemolysis assay was performed on erythrocytes .The in vitro hemolysis assay results were in concordance with earlier reports of Husain et al and Mahajan et al which showed l ‐asparaginase purified from Enterobacter cloacae and Bacillus licheniformis RAM‐8 was not toxic to human erythrocytes at higher concentrations. This result may suggest that l ‐asparaginase may exert selective cytotoxicity only on leukemic cells, making it an ideal candidate for cancer chemotherapy especially for leukemia.…”

Section: Discussionsupporting

confidence: 85%

Growth inhibitory and proapoptotic effects of l‐asparaginase from Fusarium culmorum ASP‐87 on human leukemia cells (Jurkat)

Meghavarnam

Salah

Sreepriya

et al. 2016

Fundamemntal Clinical Pharma

View full text Add to dashboard Cite

The objective of this study was to evaluate the anticancer properties of l-asparaginase purified from fungal isolate Fusarium culmorum ASP-87 against human T-cell leukemia cell line (Jurkat). The growth inhibitory and proapoptotic effects of purified l-asparaginase on Jurkat cell lines were investigated by determining its influence on cell viability, colony formation, DNA fragmentation, and cell cycle progression. The results revealed that purified l-asparaginase showed significant decrease in cell survival with IC value of 90 μg/mL (9 IU/mL). The enzyme inhibited colony formation and showed characteristic laddering pattern on agarose gel thereby confirming the induction of apoptosis. Further, cell cycle analysis revealed that the enzyme induced apoptotic cell death by arresting the growth of cells at G -M phase. However, the enzyme did not elicit any toxic effects on human erythrocytes. l-asparaginase purified from F. culmorum ASP-87 showed significant and selective cytotoxic and apoptotic effects on human T-cell leukemic cells in dose-dependent manner. Results of the study give leads for the anticancer effects of fungal l-asparaginase and its potential usefulness in the chemotherapy of leukemia.

show abstract

“…The addition of 1 or 2 to EB-bound CT-DNA solution caused obvious reduction in emission intensities, indicating complex competitively bound to CT-DNA with EB. The quenching plots illustrate that quenching of EB bound to DNA by the complexes are in agreement with the linear Stern-Volmer equation, which also indicates the complexes bind to DNA, in agreement with the change in electronic absorption data [25].…”

Section: Dna-binding Studiesmentioning

confidence: 54%

Synthesis and characterization of dinuclear (Hedta)Ru(III) complexes with N-heterocyclic ligands: magnetic properties and DNA-binding studies

Wang

Zhao

Fang

et al. 2013

Journal of Coordination Chemistry

View full text Add to dashboard Cite

Two ruthenium(III) complexes containing ethylenediaminetetraacetate(edta), viz. [{Ru(Hedta)} 2 L]· xH 2 O L = 4,4′-bipyridine(bpy) (1) and 4,4′-azopyridine(Azpy) (2), have been synthesized by the reaction between K[Ru(Hedta)Cl]·1.5H 2 O and the corresponding N-heterocycles. Complex 1 was determined by single-crystal X-ray diffraction. The products were characterized by IR, UV-vis, cyclic voltammetry, and magnetic techniques. Their DNA-binding activities were investigated using electronic absorption spectroscopic methods and fluorescence quenching; the experimental results show that these two ruthenium complexes may bind to CT-DNA through intercalation modes.

show abstract

Synthesis, DNA binding, hemolytic, and anti-cancer assays of curcumin I-based ligands and their ruthenium(III) complexes

Cited by 118 publications

References 32 publications

Synthesis and in vitro Toxicity of d‐Glucose and d‐Fructose Conjugated Curcumin–Ruthenium Complexes

Synthesis and in vitro Toxicity of d‐Glucose and d‐Fructose Conjugated Curcumin–Ruthenium Complexes

Growth inhibitory and proapoptotic effects of l‐asparaginase from Fusarium culmorum ASP‐87 on human leukemia cells (Jurkat)

Synthesis and characterization of dinuclear (Hedta)Ru(III) complexes with N-heterocyclic ligands: magnetic properties and DNA-binding studies

Contact Info

Product

Resources

About