Synthesis, DNA/HSA Interaction Spectroscopic Studies and In Vitro Cytotoxicity of a New Mixed Ligand Cu(II) Complex

Abstract

A new mixed ligand copper(II)-dipeptide complex with 2-(2'-pyridyl)benzothiazole (pbt), [Cu(Gly-L-leu)(pbt)(H2O)]·ClO4 (Gly-L-leu = Glycyl-L-leucine anion) was synthesized and characterized by various physico-chemical means. The DNA binding and cleavage properties of the complex investigated by viscosity, agarose gel electrophoresis and multi-spectroscopic techniques (UV, circular dichroism (CD) and fluorescence) showed that the complex was bound to CT-DNA through intercalation mode with moderate binding constant (K b = 3.132 × 10(4) M(-1)), and cleaved pBR322 DNA efficiently (~ 5 μM) in the presence of Vc, probably via an oxidative mechanism induced by •OH. Additionally, the interaction of the complex with human serum albumin (HSA) was explored by UV-visible, CD, fluorescence, synchronous fluorescence and 3D fluorescence spectroscopy. The complex exhibits desired affinity to HSA through hydrophobic interaction. Moreover, the cytotoxicity of the complex against three human carcinoma cell lines (HeLa, HepG2 and A549) was evaluated by MTT assay, which showed that the complex had effective cytotoxicity and higher inhibition toward A549 cell lines with IC50 of 38.0 ± 3.2 μM.

Three new mixed‐ligand copper(II) complexes containing glycyl‐l‐valine and N,N‐aromatic heterocyclic compounds: Synthesis, characterization, DNA interaction, cytotoxicity and antimicrobial activity

“…Therefore, based on the above experimental results and relevant literature, the preliminary structures of the three copper(II) complexes could be proposed with an approximate square pyramidal geometry in which four equatorial positions were occupied by Gly‐ l ‐Val (N, O) and aromatic heterocycle (N, N) and the axial position was occupied by an O atom of H 2 O as shown in Scheme .…”

“…In our previous works, we have reported a series of copper(II) peptide complexes containing N,N‐aromatic heterocyclic compounds, and also studied their DNA interactions, antioxidation properties and cytotoxicity. It was found that the ligands have significant influence on the activities.…”

Insights into the interaction of potent antimicrobial chalcone triazole analogs with human serum albumin: spectroscopy and molecular docking approaches

“…26 HSA exhibited an absorption peak at 280 nm due to the p-p* transitions of Trp, Tyr and Phe amino acids. 27 The interaction of CTs and HSA was evaluated by recording the spectra of the protein in the absence and presence of various concentrations of CTs. The incremental change in the absorption spectra ( Fig.…”

In vitro anticancer activity of parent and nano-encapsulated samarium(iii) complex towards antimicrobial activity studies and FS-DNA/BSA binding affinity

“…In addition, hydrogen bonds and van der Waals forces play a signicant role in this procedure. 43 3.3.3. Binding distance and energy transfer.…”