With their high‐surface‐to‐volume ratio, nanofibers have been postulated to increase interactions between nanofibrous materials and targeted substrates, which are helpful to overcome many obstacles and enhance the efficiency in a diverse number of applications. Over the past decade, many studies have been published on the fabrication of nanofibers and their applications in various fields. In this review, novel biological, chemical, and electrical characteristics of nanofibers as well as their recent status and achievements in medicine, chemistry, and electronics are analyzed. It is found that nanofibers can induce fast regeneration of many tissues/organs in medical applications and improve the efficiency of many chemical and electronics applications.