Synthesis, molecular features and biological activities of modified plant polysaccharides

Mukherjee, Shuvam; Jana, Subrata; Khawas, Sadhana; Kicuntod, Jintawee; Marschall, Manfred; Ray, Bimalendu; Ray, Sayani

doi:10.1016/j.carbpol.2022.119299

Cited by 60 publications

(15 citation statements)

References 382 publications

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“…6 Chemical modification with functional groups, such as carboxymethylation, sulfation, phosphorylation, and acetylation, brings new biological characteristics and expands the application of glycans. 10,11 Although there is substantial demand, it is still difficult to characterize the glycan functional groups. The common tools used for the structural characterization of glycans include nuclear magnetic resonance (NMR), 12 mass spectrometry (MS), 13 and microarrays.…”

Section: ■ Introductionmentioning

confidence: 99%

Mapping the Acetylamino and Carboxyl Groups on Glycans by Engineered α-Hemolysin Nanopores

Xia,

Fang,

et al. 2023

J. Am. Chem. Soc.

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Glycan is a crucial class of biological macromolecules with important biological functions. Functional groups determine the chemical properties of glycans, which further affect their biological activities. However, the structural complexity of glycans has set a technical hurdle for their direct identification. Nanopores have emerged as highly sensitive biosensors that are capable of detecting and characterizing various analytes. Here, we identified the functional groups on glycans with a designed αhemolysin nanopore containing arginine mutations (M113R), which is specifically sensitive to glycans with acetamido and carboxyl groups. Molecular dynamics simulations indicated that the acetamido and carboxyl groups of the glycans produce unique electrical signatures by forming polar and electrostatic interactions with the M113R nanopores. Using these electrical features as the fingerprints, we mapped the length of the glycans containing acetamido and carboxyl groups at the monosaccharide, disaccharide, and trisaccharide levels. This proof-of-concept study provides a promising foundation for developing single-molecule glycan fingerprinting libraries and demonstrates the capability of biological nanopores in glycan sequencing.

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Section: ■ Introductionmentioning

confidence: 99%

Mapping the Acetylamino and Carboxyl Groups on Glycans by Engineered α-Hemolysin Nanopores

Xia,

Fang,

et al. 2023

J. Am. Chem. Soc.

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“…Commonly the glycosides are α-1,4-glucoside, β-1,4-glucoside, and α-1,6-glucoside . Compared with animal polysaccharides and microorganism polysaccharides, plant-derived polysaccharides offer a wide range of sources and easy availability …”

Section: Natural Dietary Bioactive Compoundsmentioning

confidence: 99%

“…12 Compared with animal polysaccharides and microorganism polysaccharides, plant-derived polysaccharides offer a wide range of sources and easy availability. 13 Polysaccharides showed a large number of physiological effects, including antitumor, antiradiation, antiviral, antiinflammation, antioxidation, antifatigue, immune regulation, hypoglycemic, hypolipidemic, neuro-protection, and the gut microbiota modulation. 14−16 Polysaccharides can enter the intestine nearly intact where they become the main nutrients for gut microbial for further degradation and fermentation.…”

Section: ■ Natural Dietary Bioactive Compoundsmentioning

confidence: 99%

Neuroprotection of Food Bioactives in Neurodegenerative Diseases: Role of the Gut Microbiota and Innate Immune Receptors

Sun

2023

J. Agric. Food Chem.

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Gut-brain connections may be mediated by an assortment of microbial molecules, which can subsequently traverse intestinal and blood-brain barriers and impact neurological function. Pattern recognition receptors (PRRs) are important innate immune proteins in the gut. Gut microbiota act in concert with the PRRs is a novel target for regulating host-microbe signaling and immune homeostasis, which may involve the pathogenesis of neurodegenerative diseases. Natural food bioactives bestow a protective advantage on neurodegenerative diseases through immunomodulatory effects of the modified gut microbiota or alterations in the landscape of microbiota-produced metabolites via PRRs modulation. In this review, we discuss the effect of natural food bioactives on the gut microbiota and the role of PRRs in the gut-brain crosstalk. We focused on the neuroprotective mechanisms of natural bioactive compounds behind the action of the gut microbiota and PRRs. Research advances in natural food bioactives as antineurodegeneration agents were also presented.

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“… 2022 ; Mukherjee et al. 2022 ). These biological activities allow for their wide application in multiple diseases (Zeng et al.…”

Section: Introductionmentioning

confidence: 99%

Sanziguben polysaccharides improve diabetic nephropathy in mice by regulating gut microbiota to inhibit the TLR4/NF-κB/NLRP3 signalling pathway

Wang

Liu

Ren

et al. 2023

Pharmaceutical Biology

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Context Sanziguben (SZGB) is an empirical prescription used in traditional Chinese medicine to treat diabetic nephropathy (DN). As an abundant and primarily effective component of SZGB, Sanziguben polysaccharides (SZP) can be digested by flora to generate biological activity. Objective Our study aimed to clarify the potential mechanism of SZP in improving chronic DN. Materials and methods Male db/db mice were randomized into DN, SZP (500 mg/kg) and metformin (MET, 300 mg/kg) groups. Wild-type littermates served as the normal control (NC) group. The drug was orally administered for 8 weeks. Enzyme-linked immunosorbent assay was used to detect the inflammatory factors. Proteins related to inflammation were evaluated using western blotting and immunohistochemical examination. Gut microbiota was analysed using 16S rRNA sequencing. Results SZP significantly reduced 24 h urine albumin ( p < 0.05) of DN mice. Compared to DN group, SZP significantly decreased the homeostasis model assessment of insulin resistance index, serum creatinine and blood urea nitrogen levels (20.27 ± 3.50 vs. 33.64 ± 4.85, 19.22 ± 3.77 vs. 32.52 ± 3.05 μmol/L, 13.23 ± 1.42 vs. 16.27 ± 0.77 mmol/L, respectively), and mitigated renal damage. SZP also regulated gut microbiota and decreased the abundance of Gram-negative bacteria (Proteobacteria, Klebsiella and Escherichia-Shigella ). Subsequently, SZP reduced lipopolysaccharides levels (1.06- to 1.93-fold) of DN mice. Furthermore, SZP inhibited the expression levels of TLR4, phospho-NF-κB p65, NLRP3 proteins and interleukin (IL)-18 and IL-1β. Conclusions These results demonstrated that SZP improved intestinal flora disorder and inhibited the TLR4/NF-κB/NLRP3 pathway to alleviate DN.

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Synthesis, molecular features and biological activities of modified plant polysaccharides

Cited by 60 publications

References 382 publications

Mapping the Acetylamino and Carboxyl Groups on Glycans by Engineered α-Hemolysin Nanopores

Mapping the Acetylamino and Carboxyl Groups on Glycans by Engineered α-Hemolysin Nanopores

Neuroprotection of Food Bioactives in Neurodegenerative Diseases: Role of the Gut Microbiota and Innate Immune Receptors

Sanziguben polysaccharides improve diabetic nephropathy in mice by regulating gut microbiota to inhibit the TLR4/NF-κB/NLRP3 signalling pathway

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