Synthesis of Biocompatible and Environmentally Nanofibrous Mats Loaded with Moxifloxacin as a Model Drug for Biomedical Applications

Abstract

Biopolymeric chitosan structure (Cs) is rationally investigated owing to its potentiality in pharmaceutical applications. The synthetic routes of biomimetic Cs-based blend electrospun nanofibers were studied. Herein, biocompatible crosslinked electrospun polyvinyl alcohol (PVA)/Cs-reduced gold nanoparticles (Cs(Rg))/β-CD (beta-cyclodextrin) in pure water were fabricated. To this end, supportive PVA as a carrier, Cs bio modifier, and gold reductant and β-CD as smoother, inclusion guest molecule, and capping agent exhibit efficient entrapment of moxifloxacin (Mox) and consequently accelerate release. Besides, PVA/Cs(Rg)/β-CD paves towards controlled drug encapsulation-release affinity, antimicrobial, and for wound dressing. Without losing the nanofiber structure, the webs prolonged stability for particle size and release content up to 96.4%. The synergistic effect of the nanoformulation PVA/Cs(Rg)/β-CD against pathogenic bacteria, fungus, and yeast, including Staphylococcus aureus, Escherichia coli, Candida albicans, and Aspergillus niger, posed clear zones up to 53 φmm. Furthermore, a certain combination of PVA/Cs (Rg)/β-CD showed a total antioxidant capacity of 311.10 ± 2.86 mg AAE/g sample. In vitro cytotoxicity assay of HePG2 and MCF-7 NF6 can eradicate 34.8 and 29.3 µg/mL against selected cells.

Chitosan based-nanoparticles and nanocapsules: Overview, physicochemical features, applications of a nanofibrous scaffold, and bioprinting

“…In general, the polycationic nature of the chitosan molecule communicates with the components of the microorganism's anionic surface antigens (lipopolysaccharides and proteins), which contributes to the release of intracellular response: to fluctuations in the permeability barrier. Also, it prevents bacterial growth by stopping the entry of nutrients into the cell; once in the cell, binds to DNA and thus inhibits the synthesis of proteins and RNA [44,47,48].…”

Electrospun Nanofiber Scaffolds Loaded with Metal-Based Nanoparticles for Wound Healing

“…Then, these biogenic AuNPs were combined with drugs such as moxifloxacin hydrochloride, peppermint oil, and a nanoemulsion (SNE) to produce antibacterial and anti-inflammatory nanofibers. This approach provides a new strategy and method for the development of efficient and environmentally friendly wound healing materials [ 162 , 163 ].…”

Synthesis of Cross-Linked and Sterilized Water-Soluble Electrospun Nanofiber Biomatrix of Streptomycin-Imbedded PVA/CHN/β-CD for Wound Healing

“…To ensure minimal side effects, controlled medications from various periods are precisely administered to the specific target area . Among solvents, water stands out as the optimal choice for electrospinning nanofibers due to its nontoxicity, cytocompatibility, and universal applicability. , The skin acts as a crucial barrier against microbes; therefore, any skin injury demands appropriate wound care for hemostasis, healing, and safeguarding the body from external elements. , Wound dressing materials must meet stringent criteria; they should be nontoxic, nonallergenic, and create a conducive environment for accelerated wound healing.…”

“…Besides, the polycationic structure of CHN causes its solution to be extremely viscous, making electrostatic spinning challenging . By combining CHN with other synthetic biocompatible polymers such as poly­(vinyl alcohol) (PVA), these limitations can be overcome. ,, PVA has many beneficial properties, including its ability to form fibers, degrade quickly, and absorb moisture. PVA nanofibers can absorb the exudates in infected wounds and enhance the regeneration of wound tissues due to their nontoxicity, biocompatibility, and oxygen permeability .…”