Synthesis of biocompatible Konjac glucomannan stabilized silver nanoparticles, with Asystasia gangetica phenolic extract for colorimetric detection of mercury (II) ion

Abstract

Herein, the synthesis of a biocompatible silver nanoparticles (AgNPs), for colorimetric detection of toxic mercury (II) ion (Hg2+), is reported. Phenolic-rich fraction of Asystasia gangetica leaf was extracted and used as a reductant of silver salt, all within the hydrophilic konjac glucomannan (KgM) solution as stabilizer, at room temperature (RT). The bioactive components of Asystasia gangetica phenolic extract (AGPE), as elucidated with a (UHPLC-MS-QTOF-MS), revealed plethora of phenolic compounds, which can facilitate the reduction of silver salt at ambient conditions. Sparkling yellow colloidal solution of KgM-AgNPs was realized within 1 h, at RT, having a UV–vis maximum at 420 nm. KgM-AgNPs was characterized using UV–vis, Raman and (FTIR), TEM, SEM, EDS, XRD, TGA/DTG. TEM and FESEM images showed that KgM-AgNPs were spherical, with particle size distribution around 10–15 nm from TEM. The KgM-AgNPs biocompatibility was investigated on mouse L929 fibrobroblast and rat erythrocytes, without any harmful damages on the tested cells. In aqueous environment, KgM-AgNPs demonstrated good detection capacity toward Hg2+, in a Hg2+ concentration dependent fashion, within 3 min. Absorbance ratios (A360/A408) was linear with Hg2+ concentrations from 0.010–10.0 to 10.0–60.0 µM, with an estimated (LOD) of 3.25 nM. The probe was applied in lake water sample, with satisfactory accuracy.

Faba Bean (Vicia faba L. minor) Bitterness: An Untargeted Metabolomic Approach to Highlight the Impact of the Non-Volatile Fraction

“…It was not possible to tentatively identify the compounds ( 40 ) and ( 42 ) because their fragmentation data were not available in the literature. The compound ( 40 ) could be a diterpene glycoside called 19-hydroxycinnzeylanol 19-glucoside, which has already been identified in the leaf of a dicotyledon plant [ 46 ]. Concerning the compound ( 42 ), the MS 1 spectrum was consistent with those of 4-chloro-oxoindole-acetic acid, which have been detected in faba bean seeds [ 21 ].…”

Preparation and usage of nanomaterials in biomedicine

“…As previously reported, SeNPs were embedded into β‐(1,3)‐ d ‐glucan (BFP) extracted from Auricularia auricula, which formed stable and well‐dispersed hollow BFP–SeNPs nanotubes in water, which can kill acute myeloid leukemia (AML) cells (Jin et al, 2020). Jayeoye et al (2022) used Asystasia gangetica phenolic extract as the reducing agent of silver salt and konjac glucomannan (KgM) as the stabilizer of AgNPs to prepare KgM–AgNPs, which can be used as a colorimetric probe to detect toxic Hg 2+ .…”

“…Marina et al developed a new multifunctional nanodevice, which uses adriamycin-loaded silica NPs as the nanoplatforms and can use concanthus bean lectin as the targeting ligand to improve biocompatibility(Martínez-Carmona et al, 2018).PolysaccharideThe polysaccharide can stabilize the NPs. As previously reported, SeNPs were embedded into β-(1,3)-D-glucan (BFP) extracted from Auricularia auricula, which formed stable and well-dispersed hollow BFP-SeNPs nanotubes in water, which can kill acute myeloid leukemia (AML) cells(Jin et al, 2020) Jayeoye et al (2022). used Asystasia gangetica phenolic extract as the reducing agent of silver salt and konjac glucomannan (KgM) as the stabilizer of AgNPs to prepare KgM-AgNPs, which can be used as a colorimetric probe to detect toxic Hg 2+ .CompoundsAgNPs reduce Ag ions to metal NPs by using different compounds, such as flavonoids, ketones, aldehydes, tannins, carboxylic acids, and phenols from plant extracts, making them low toxic and environmentally friendly(Rajan et al, 2015) Luhata et al (2022).…”

Green Synthesized Silver Nanoparticles: A Potential Antibacterial Agent, Antioxidant, and Colorimetric Nanoprobe for the Detection of Hg2+ Ions

“…[ 3 , 4 ] AgNPs have numerous applications, such as in optics, [ 5 , 6 ] selective coating, [ 7 ] water purification, [ 8 ] catalysis, [ 9 , 10 , 11 , 12 , 13 ] biolabeling, [ 14 ] biomedicine, [ 15 ] food chemistry, [ 16 ] agriculture, [ 17 ] cosmetics, [ 18 ] food packaging, [ 19 ] and sensing. [ 5 , 20 , 21 , 22 , 23 , 24 ] They are extensively researched as antioxidant, [ 25 , 26 , 27 , 28 , 29 ] antibacterial, [ 25 , 26 , 30 , 31 , 32 ] antifungal, [ 33 , 34 ] anti‐inflammatory, [ 35 ] antiviral, [ 36 ] and anticancer substances [ 25 , 26 , 37 , 38 ] in the field of pharmacology. AgNPs are widely known for their potent antibacterial activity [ 8 , 39 , 40 , 41 ] and their incredibly cost‐effective and selective colorimetric sensor for the detection of metal ions.…”