Synthesis of Biologically Active Gibberellins GA4 and GA7 by Microorganisms

Пирог, Т. П.; Havrylkina, D.V.; Leonova, N.O.; Shevchuk, T.A.; Iutynska, G.O.

doi:10.15407/microbiolj81.02.090

Cited by 5 publications

(4 citation statements)

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“…Previous studies have established the ability of the surfactants producer Rhodococcus erythropolis IMV Ac-5017 to synthesize stimulatory phytohormones -auxins, cytokinins and gibberellins [5,6]. In our opinion, such results may be promising for the development of metabolic preparations with growth-stimulating properties for possible use in plant production.…”

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confidence: 83%

Untitled

2020

Mikrobiol. Z.

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The ability of surfactant producers to synthesize phytohormones expands the scope of their practical application and provides prospects for the development of microbial preparations with growth-stimulating properties. The possibility to intensify the phytohormone-stimulants synthesis by bacterial strains increases the efficiency of such preparations. Aim. The aim is to research the possibility of extracellular auxin synthesis induction in the presence of tryptophan in the cultivation medium of surfactant producer Rhodococcus erythropolis IMV Ac-5017 and establish the optimal concentration of tryptophan and time of introduction into the medium to ensure maximum synthesis of auxins. Methods. Biochemical, microbiological, biotechnological. Cultivation was performed in the liquid mineral medium using ethanol and waste sunflower oil as substrates. Tryptophan was added to the medium as a 1 % solution in an amount of 200 or 300 mg/l at the beginning of the cultivation process or at the end of the exponential growth phase. Phytohormones were isolated by triple extraction with organic solvents from the culture broth supernatant after surfactant extraction. Preliminary purification and concentration of phytohormones was performed by thin layer chromatography. Qualitative and quantitative determination of auxins was performed using high performance liquid chromatography. Results. It was found that regardless of the concentration and time of tryptophan introduction to the culture medium of R. erythropolis IMV Ac-5017 with both substrates, a significant increase (by two to three orders of magnitude) was observed in the amount of synthesized auxins compared to tryptophan-free medium. The highest concentration of auxins (5552-5634 μg/l) was achieved by adding 300 mg/l of tryptophan into the culture medium of R. erythropolis IMV Ac-5017 with ethanol, while without the precursor their amount was only 143 μg/l. In contrast to the cultivation of the strain on culture medium with ethanol, where the synthesis of auxins did not depend on the time of tryptophan introduction, R. erythropolis IMV Ac-5017 formed the maximum amount of auxins when 300 mg/l tryptophan was added to the culture medium with waste oil at the end of the exponential growth phase (2398 μg/l compared to 9.8 μg/l on the medium without tryptophan). As auxin compounds were identified: indole-3-acetic acid, indole-3-carboxylic acid and indole-3-butyric acid. However, the highest amount of indole-3-acetic acid was synthesized, the precursor of which is tryptophan. The synthesis of this auxin (the most common plant auxin) in the presence of 300 mg/l of tryptophan increased more than 40 times on ethanol medium and more than 700 times on medium with waste oil. Induction of auxin synthesis by strain R. erythropolis IMV Ac-5017 correlated with the activity of tryptophan transaminase: when cultured on ethanol without tryptophan, it was 138 nmol•min -1 •mg -1 of protein, while cultured in the presence of precursor it was increased by 5.2 times (up to 714 nmol•min -1 •mg -1 of...

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confidence: 83%

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2020

Mikrobiol. Z.

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“…Phytohormones. Bacteria of the Acinetobacter genus are common in the soil, some of them are associated with plants, have a positive effect on their growth and development, including due to the ability to synthesize phytohormones [21,22,[85][86][87][88][89][90].…”

Section: Synthesis Of Practically Important Metabolites By Acinetobacter Genus Bacteriamentioning

confidence: 99%

“…Our studies [89,90] have shown that A. calcoaceticus IMV B-7241 strain, when culti vated on various substrates, including industrial waste (technical glycerol, biodiesel production waste), synthesizes phytohormones of auxin, cytokinin and gibberellin nature. During cultivation on ethanol and technical glycerol, the concentration of phytohormones (µg/l) was: auxins − 104.2 and 122.0; cytokinins -3.5 and 363.9 [90]; biologically active gibberellin GK 4 − 6.88 and 7.36 [90].…”

Section: Synthesis Of Practically Important Metabolites By Acinetobacter Genus Bacteriamentioning

confidence: 99%

“…It should be noted that the ability of A. calcoaceticus IMV B-7241 strain to the simultaneous synthesis of phytohormones of auxin, cytokinin and gibberellin nature [89][90][91], as well as biosurfactants with antibacterial activity against phytopathogenic bacteria [92] makes it promising for use in agriculture to increase plant yield and phytopathogenic bacteria quantity control.…”

Section: Synthesis Of Practically Important Metabolites By Acinetobacter Genus Bacteriamentioning

confidence: 99%

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Biotechnological Potential of the Acinetobacter Genus Bacteria

Пирог¹,

Lutsai²,

Muchnyk³

2021

Mikrobiol. Z.

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Until recently, there were rare scientific reports on the biotechnological potential of non-pathogenic bacteria of the Acinetobacter genus. Although the first reports about the practically valuable properties of these bacteria date back to the 70s and 80s of the twentieth century and concerned the synthesis of the emulsan bioemulsifier. In the last decade, interest in representatives of the Acinetobacter genus as objects of biotechnology has significantly increased. The review presents current literature data on the synthesis by bacteria of this genus of high-molecular emulsifiers, low-molecular biosurfactants of glyco- and aminolipid nature, enzymes (lipase, agarase, chondroitinase), phytohormones, as well as their ability to solubilize phosphates and decompose various xenobiotics (aliphatic and aromatic hydrocarbons, pesticides, insecticides). Prospects for practical application of Acinetobacter bacteria and the metabolites synthesized by them in environmental technologies, agriculture, various industries and medicine are discussed. The data of own experimental studies on the synthesis and biological activity (antimicrobial, anti-adhesive, ability to destroy biofilms) of biosurfactants synthesized by A. calcoaceticus IMV B-7241 strain and their role in the degradation of oil pollutants, including complex ones with heavy metals, are presented. The ability of A. calcoaceticus IMV B-7241 to the simultaneous synthesis of phytohormones (auxins, cytokinins, gibberellins) and biosurfactants with antimicrobial activity against phytopathogenic bacteria allows us to consider this strain as promising for practical use in crop production to increase crop yields.

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