Synthesis of cationic magnetite nanoparticles for intracellular protein delivery

Abstract

Intracellular protein delivery shows great promise in the treatment of various diseases. However, therapeutic applications of this method are limited by its low delivery efficiency and poor targeting ability. As one of most important drug delivery cargoes, Fe3O4 nanoparticles (nFe3O4) have attracted much attention for both therapeutic and diagnostic applications, especially for targeting drug delivery. To use nFe3O4 for protein delivery, a simple but effective modification of nFe3O4 is critical to attach proteins on its surface. In this work, by designing and synthesizing cationic poly(2‐(dimethylamino)ethyl methacrylate) (PDMA)‐grafted nFe3O4 via in situ atom transfer radical polymerization (ATRP), we demonstrate a simple solution to improve interactions between nFe3O4 and proteins. With the grafted PDMA on the surface, nFe3O4 exhibits not only significant enhancement in dispersibility and stability in aqueous phase, but also an excellent capability to attach negative‐charged proteins. Moreover, with the assistance of external magnetic field, PDMA‐grafted nFe3O4 can be used as a targetable vector to deliver proteins into specific cells. This work provides a novel platform based on cationic magnetite nanoparticles that can deliver therapeutic proteins into specific sites for the treatment of various diseases. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40260.

Transduction Methods for Cytosolic Delivery of Proteins and Bioconjugates into Living Cells

“…Cell apoptosis was observed only when a magnetic field was used, demonstrating cytosolic delivery of caspase 3. Other magnetic protein carriers were produced by classical methods, either by covalent or noncovalent linking of polymers onto the surface of SPIO . These carriers transduced BSA and GFP inside the cytosol in a magnetic field‐dependent manner.…”

“…Other magnetic protein carriers were produced by classical methods, either by covalent or noncovalent linking of polymers onto the surface of SPIO. [165] These carriers transduced BSA and GFP inside the cytosol in a magnetic field-dependent manner. Nevertheless, the use of SPIO nanoparticles for large molecule transduction requires improved delivery efficiency, including strategies to reduce/ avoid long term SPIO nanoparticles accumulation in animal, favoring biological elimination.…”

Failure mechanism of rigid polyurethane foam under high temperature vibration condition by experimental and finite element method

“…The experimental results revealed that compressive strength of polyurethane foam decreased significantly after degradation, and the ester/hydroxyl band ratio was a good indicator to assess the degradation degree. Wang et al . investigated the thermal decomposition kinetics of RPUF in oxidative atmosphere.…”

Smoldering spot ignition of natural fuels by a hot metal particle

“…More elaborate 1-D models have also been developed for flaming ignition [28]. A related problem is the spot fire ignition of polymer foams found in industrial and urban environments has also been studied [29][30][31].…”