Abstract:The cerium oxide nanoparticles (CeO 2 NPs) and M 2+ doped CeO 2 NPs (M 2+ = Zn 2+ , Ni 2+ , Cu 2+ and Co 2+ ) were prepared through the Azadirachta indica (A. indica) leaf extracts. The x-ray diffraction studies confirmed that synthesized pure and M 2+ doped CeO 2 NPs were exhibited the cubic structure. The FESEM and TEM images of both pure and M 2+ doped CeO 2 NPs have exhibited spherical structure. From the EDAX spectral analysis, the elemental compositions were identified. The various functional groups were confirmed by FT-IR spectrum. Photoluminescence spectral analysis, small shift observed for emission of the CeO 2 NPs values as compared to that of the transition metal ions doped CeO 2 NPs emission values. The antimicrobial studies were performed against a set of gram positive (Staphylococcus aureus and Streptococcus pneumoniae) and gram negative (Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumonia and Shigelladysenteriae) bacterial and fungal candida albicans strain.