Synthesis of chitosan-alginate microspheres with high antimicrobial and antibiofilm activity against multi-drug resistant microbial pathogens

Thaya, Rajagopalan; Vaseeharan, Baskaralingam; Sivakamavalli, Jeyachandran; Iswarya, Arokiadhas; Govindarajan, Marimuthu; Alharbi, Naiyf S.; Kadaikunnan, Shine; Al-anbr, Mohammed N.; Khaled, Jamal M.; Benelli, Giovanni

doi:10.1016/j.micpath.2017.11.011

Cited by 61 publications

(45 citation statements)

References 42 publications

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“…Similarly, it is reported that chitosan (2.6 kDa) at > 1 mg/ml prevents biofilms formation of LM, PA, and SA [17]. Also, alginate, a carboxyl methyl derivative of chitosan with varying degrees of efficiency was reported to inhibit biofilms formation in pathogens [18]. However, the antibiofilm effect of CFA observed in our study is more effective as compared to the antibioiflm property reported for catechol derivatives of chitosan against S.…”

supporting

confidence: 61%

Antibacterial and Biofilm Modulating Potential of Ferulic Acid Grafted Chitosan against Human Pathogenic Bacteria

Dasagrandhi

Park

Jung

et al. 2018

Preprint

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Emergence of more virulent forms of human pathogenic bacteria with multi drug resistance is a serious global issue and requires alternative control strategies. The current study was focused to investigate the antibacterial and antibiofilm potential of ferulic acid grafted chitosan (CFA) against Listeria monocytogenes (LM), Pseudomonas aeruginosa (PA), and Staphylococcus aureus (SA). The present result showed that CFA at 64 µg/mL concentration exhibit bactericidal action against LM and SA (>4 log reduction) and bacteriostatic action against PA (<2 log CFU) within 24 h of incubation. Further studies based on propidium iodide uptake assay, measurement of material released from the cell, and electron microscopic analysis revealed that the bactericidal action of CFA was due to the altered membrane integrity and permeability. CFA dose-dependently inhibited biofilm formation (52-89% range), its metabolic activity (30.8-75.1% range) and eradicated mature biofilms, and reduced viability (71-82% range) of the test bacteria. Also, the swarming motility of LM was differentially affected at sub-MIC concentration of CFA. In the present study, the ability of CFA to kill and alter the virulence production in human pathogenic bacteria will insight a new scope for the application of these biomaterials in healthcare to effectively treat bacterial infections.

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supporting

confidence: 61%

Antibacterial and Biofilm Modulating Potential of Ferulic Acid Grafted Chitosan against Human Pathogenic Bacteria

Dasagrandhi

Park

Jung

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Similarly, it is reported that chitosan (2.6 kDa) at >1 mg/mL prevents biofilms formation of LM, PA, and SA [17]. Also, alginate, a carboxyl methyl derivative of chitosan with varying degrees of efficiency was reported to inhibit biofilm formation in pathogens [18]. However, the antibiofilm effect of CFA observed in our study is more effective compared to the antibioiflm property reported for catechol derivatives of chitosan against S. epidermidis biofilms [19].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Biofilm Modulating Potential of Ferulic Acid-Grafted Chitosan against Human Pathogenic Bacteria

Dasagrandhi

Park

Jung

et al. 2018

IJMS

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The emergence of more virulent forms of human pathogenic bacteria with multi-drug resistance is a serious global issue and requires alternative control strategies. The current study focused on investigating the antibacterial and antibiofilm potential of ferulic acid-grafted chitosan (CFA) against Listeria monocytogenes (LM), Pseudomonas aeruginosa (PA), and Staphylococcus aureus (SA). The result showed that CFA at 64 µg/mL concentration exhibits bactericidal action against LM and SA (>4 log reduction) and bacteriostatic action against PA (<2 log colony forming units/mL reduction) within 24 h of incubation. Further studies based on propidium iodide uptake assay, measurement of material released from the cell, and electron microscopic analysis revealed that the bactericidal action of CFA was due to altered membrane integrity and permeability. CFA dose dependently inhibited biofilm formation (52–89% range), metabolic activity (30.8–75.1% range) and eradicated mature biofilms, and reduced viability (71–82% range) of the test bacteria. Also, the swarming motility of LM was differentially affected at sub-minimum inhibitory concentration (MIC) concentrations of CFA. In the present study, the ability of CFA to kill and alter the virulence production in human pathogenic bacteria will offer insights into a new scope for the application of these biomaterials in healthcare to effectively treat bacterial infections.

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“…[22][23][24][25][26][27] Thaya et al demonstrated antibacterial activity of Ca 2+ cross-linked alginate/chitosan microspheres. 28 El-Aassar and Mo tested antibacterial activity of alginate microbeads containing silver nanoparticles (AgNPs). 29 Zahran et al modied surface of cotton fabric with AgNPs-alginate composite and evaluated their antibacterial activity.…”

Section: Introductionmentioning

confidence: 99%

Polydopamine functionalized hydrogel beads as magnetically separable antibacterial materials

et al. 2019

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Synthesis of chitosan-alginate microspheres with high antimicrobial and antibiofilm activity against multi-drug resistant microbial pathogens

Cited by 61 publications

References 42 publications

Antibacterial and Biofilm Modulating Potential of Ferulic Acid Grafted Chitosan against Human Pathogenic Bacteria

Antibacterial and Biofilm Modulating Potential of Ferulic Acid Grafted Chitosan against Human Pathogenic Bacteria

Antibacterial and Biofilm Modulating Potential of Ferulic Acid-Grafted Chitosan against Human Pathogenic Bacteria

Polydopamine functionalized hydrogel beads as magnetically separable antibacterial materials

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