Synthesis of colloidal silver nanoparticle clusters and their application in ascorbic acid detection by SERS

Cholula-Díaz, Jorge L.; Lomelí-Marroquín, Diana; Pramanick, Bidhan; Nieto-Argüello, Alfonso; Cantú-Castillo, Luis A.; Hwang, Hyundoo

doi:10.1016/j.colsurfb.2017.12.051

Cited by 49 publications

(27 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Starch powder was directly extracted from potatoes, and its chemical composition was determined by an enzymatic method revealing a 22.41±0.51 wt% amylose content. 47 All experiments were completed using deionized water (σ=18.2 MΩ cm). For cell fixation studies, poly-llysine, cacodylate buffer, glutaraldehyde, liquid graphite and osmium tetroxide (OsO 4 ) were all purchased from EMS (Electron Microscopy Sciences, Hatfield, PA, USA).…”

Section: Materials and Methods Chemicalsmentioning

confidence: 99%

“…The synthesis of colloidal AuNPs and Ag/Au alloy NPs (or simply Ag/AuNPs) was carried out using an improved method based on a previous work. 47 Briefly, a 1% w/v starch solution was prepared using boiling water. The starch solution was heated under constant reflux and stirred for 30 minutes.…”

Section: Synthesis Of Colloidal Noble Metal Npsmentioning

confidence: 99%

“…spectroscopy, which is an analytical technique that allows the estimation of concentration, degree of agglomeration and particle size of NPs stabilized in suspension. 47,53,55 In Figure 3, we show the UV-visible absorption spectra for six sample batches that were independently synthesized for each colloidal metallic system. The maximum wavelength of the absorption bands (λ max ) was observed at 462±1 and 524±2 nm for Ag/AuNPs and AuNPs, respectively.…”

Section: Determination Of Starch As a Capping Agent By Ft-ir Spectrosmentioning

confidence: 99%

See 2 more Smart Citations

<p>Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents</p>

Lomelí-Marroquín

Medina-Cruz

Nieto-Argüello

et al. 2019

IJN

Self Cite

118

View full text Add to dashboard Cite

Background and aim: Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells. Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent. Methods: The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and ζ-potential. The presence of starch as capping agent was determined by Fourier transform infrared (FT-IR) spectroscopy. The structural properties were studied by X-ray diffraction (XRD). Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures. The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis. To further study the biomedical applications of the synthesized nanostructures, antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) were conducted. In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment. Results: UV-visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs. The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively. The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior. Furthermore, the NPs showed cytocompatibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours. Conclusion: In this study, mono-and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications.

show abstract

Section: Materials and Methods Chemicalsmentioning

confidence: 99%

Section: Synthesis Of Colloidal Noble Metal Npsmentioning

confidence: 99%

Section: Determination Of Starch As a Capping Agent By Ft-ir Spectrosmentioning

confidence: 99%

See 1 more Smart Citation

<p>Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents</p>

Lomelí-Marroquín

Medina-Cruz

Nieto-Argüello

et al. 2019

IJN

Self Cite

118

View full text Add to dashboard Cite

show abstract

“…To evaluate the SERS enhancement effect of the AuNPs@PP-SH devices for the detection of PC in aqueous fluids, we prepared a quaternary mixture containing PC (1 mM), DA (1 mM), UA (1 mM), and AA (1 mM) in PBS buffer solution and tested it on the chips using SERS spectroscopy with comparison to the mixture solutions of [HA (1 mM) + DA (1mM) + UA (1 mM) + AA (1 mM)] and [IS (1 mM) + DA (1mM) + UA (1 mM) + AA (1 mM)] (Figure 9). Previous reports have shown that the strongest peak at 1331, 1386, and 1567 cm −1 can be chosen to estimate the correlation between the Raman intensity and the concentration of DA, AA, and UA, respectively (Pucetaite et al, 2016; Cholula-Díaz et al, 2018; Phung et al, 2018). The AuNPs@PP-SH devices provided a higher-intensity Raman signal for PC, due to its greater SERS activity (SERS hot spots), in the presence of DA, UA, and AA than did the PP-SH devices; nevertheless, discrimination of the overlapping region of signals from the DA, AA, and UA analytes from the background signals of AuNPs@PP-SH devices remained a major challenge in the fingerprint region (1300–1600 cm −1 ) (Figure 9h).…”

Section: Resultsmentioning

confidence: 99%

Organic Electrochemical Transistors/SERS-Active Hybrid Biosensors Featuring Gold Nanoparticles Immobilized on Thiol-Functionalized PEDOT Films

Chou

Chung

et al. 2019

Front. Chem.

View full text Add to dashboard Cite

In this study we immobilized gold nanoparticles (AuNPs) onto thiol-functionalized poly(3,4-ethylenedioxythiophene) (PEDOT) films as bioelectronic interfaces (BEIs) to be integrated into organic electrochemical transistors (OECTs) for effective detection of dopamine (DA) and also as surface-enhanced Raman scattering (SERS)—active substrates for the selective detection of p -cresol (PC) in the presence of multiple interferers. This novel PEDOT-based BEI device platform combined (i) an underlying layer of polystyrenesulfonate-doped PEDOT (PEDOT:PSS), which greatly enhanced the transconductance and sensitivity of OECTs for electrochemical sensing of DA in the presence of other ascorbic acid and uric acid metabolites, as well as amperometric response toward DA with a detection limit (S/N = 3) of 37 nM in the linear range from 50 nM to 100 μM; with (ii) a top interfacial layer of AuNP-immobilized three-dimensional (3D) thiol-functionalized PEDOT, which not only improved the performance of OECTs for detecting DA, due to the signal amplification effect of the AuNPs with high catalytic activity, but also enabled downstream analysis (SERS detection) of PC on the same chip. We demonstrate that PEDOT-based 3D OECT devices decorated with a high-density of AuNPs can display new versatility for the design of next-generation biosensors for point-of-care diagnostics.

show abstract

“…Currently available methods for the detection of AA and ALP activity include surface‐enhanced Raman scattering, electrochemical methods, fluorescence, and colorimetric assay . Among these, colorimetric assay displays obvious advantages due to its fast response, a simple, non‐time‐consuming process, low cost and easy readout with the naked eye.…”

Section: Introductionmentioning

confidence: 99%

Redox‐modulated colorimetric detection of ascorbic acid and alkaline phosphatase activity with gold nanoparticles

et al. 2020

View full text Add to dashboard Cite

Gold nanoparticles (AuNPs) exhibit characteristic absorption peaks in the ultraviolet visible region due to their special surface plasmon resonance effect. This characteristic absorption peak would change with the relative colour varying from wine red to orange-yellow upon sequential addition of ascorbic acid (AA) into the mixture of AuNPs and Ag(I). Similar observations also could be found when the hydrolysis product of sodium L-ascorbyl-2-phosphate with alkaline phosphatase (ALP) was used as an alternative to AA. Results of structure characterization confirmed that the phenomena were due to the reduction of Ag(I) to Ag(0) on the surface of AuNPs and the formation of core-shell AuNPs@Ag. Therefore, a colorimetric assay for rapid visual detection of AA and ALP based on redox-modulated silver deposition on AuNPs has been proposed. Under the optimal experimental conditions, the absorbance variation ΔA 522 nm /A 370 nm of AuNPs was proportional to the concentration of AA (5--60 μmol/L) and ALP (3-18 U/L) with the corresponding detection limit of 2.44 μmol/L for AA and 0.52 U/L for ALP. The assay showed excellent selectivity towards AA and ALP. Moreover, the assay has been applied to detect AA and ALP activity in real samples with satisfying results. K E Y W O R D S alkaline phosphatase, ascorbic acid, core-shell AuNPs@Ag, redox-modulated silver deposition, sodium L-ascorbyl-2-phosphate /journal/bio F I G U R E 5 (a) UV-Vis spectra of AuNPs-Ag(I)-AA system with the addition of AA range 0-90 μmol/L. Inset: Corresponding R A change with different AA concentrations. (b) Linear relationship between ΔR A and the AA concentration in the range 5-60 μmol/L. Inset: Corresponding colour change of AuNPs-Ag(I) system with different AA concentrations. (c) UV-Vis spectra of AuNPs-Ag(I)-AA-P-ALP system with the addition of ALP range 0-25 U/L. Inset: Corresponding R A change with different ALP concentrations. (b) Linear relationship between ΔR A and the concentration of ALP in the range 3-18 U/L. Inset: Corresponding colour change of AuNPs-Ag(I)-AA-P system with different ALP concentrations LIN ET AL. 547

show abstract

Synthesis of colloidal silver nanoparticle clusters and their application in ascorbic acid detection by SERS

Cited by 49 publications

References 42 publications

<p>Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents</p>

<p>Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents</p>

Organic Electrochemical Transistors/SERS-Active Hybrid Biosensors Featuring Gold Nanoparticles Immobilized on Thiol-Functionalized PEDOT Films

Redox‐modulated colorimetric detection of ascorbic acid and alkaline phosphatase activity with gold nanoparticles

Contact Info

Product

Resources

About