Synthesis of Desoxyribonucleic Acid in Normal and Irradiated Cells and Its Relation to Chromosome Breakage

Howard, Alma; Pelc, S. R.

doi:10.1080/09553008514552501

Cited by 133 publications

(98 citation statements)

References 14 publications

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“…A11 analyses were performed using peak height detection and logarithmic amplification . The amount of DNA in the nuclei is proportional to the fluorescent signal and is expressed as arbitrary C values (Howard and Pelc, 1953). The signals obtained from tomato leaf tissue were used, and the gain settings were adjusted so that signals of a11 intact nuclei were registered within the channel range.…”

Section: Flow Cytometry Of Nucleimentioning

confidence: 99%

[beta]-Tubulin Accumulation and DNA Replication in Imbibing Tomato Seeds

et al. 1995

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The activation of the cell cycle in embryo root tips of imbibing tomato (Lycopersicon esculentum Mill. cv Lerica) seeds was studied by flow cytometric analyses of the nuclear DNA content and by immunodetection of p-tubulin. With dry seeds, flow cytometric profiles indicated that the majority of the cells were arrested at the C , phase of the cell cycle. In addition, p-tubulin was not detectable on western blots. Upon imbibition of water, the number of cells in C , started to increase after 24 h, and a 55-kD fl-tubulin signal was detected between 24 and 48 h. Two-dimensional immunoblots revealed at least three different p-tubulin isotypes. Thus, p-tubulin accumulation and DNA replication were induced during osmotic priming. These processes, as well as seed germination rate, were enhanced upon subsequent imbibition of water, compared with control seeds that imbibed but were not primed. By contrast, when aged seeds imbibed, DNA replication, P-tubulin accumulation, and germination were delayed. In all cases studied, both DNA replication and p-tubulin accumulation preceded visible germination. We suggest that activation of these cell-cycle-related processes is a prerequisite for tomato seed germination. Furthermore, p-tubulin expression can be used as a parameter for following the initial processes that are activated during seed imbibition.

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Section: Flow Cytometry Of Nucleimentioning

confidence: 99%

[beta]-Tubulin Accumulation and DNA Replication in Imbibing Tomato Seeds

et al. 1995

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“…Although displacement of labelled cells away from the tip was treated as a feature of cell kinetics at a later date (Barlow 1985), Howard and Pelc (1951c) immediately realised that it was possible thereby not only to estimate the cycle time of cells within the meristem, but also to infer that there were two periods during interphase when nuclear DNA was not being synthesised. These became known as the G1 and G2 phases of the cycle (Howard and Pelc 1953). Thus, it was now possible, using continuous labelling with the more specific DNA precursor, 3 H-thymidine (which also gave a superior resolution to the autoradiographs due to the shorter path-length of the β-particles), to estimate the timing of the mitotic cycle in various zones of the Zea apex (Clowes 1961b).…”

Section: Cell Kinetics In the Qc And Meristem Before And After X-irramentioning

confidence: 99%

The concept of the quiescent centre and how it found support from work with X-rays. I. Historical perspectives

Barlow

2015

Plant Root

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“…She (with Stephen Pelc) was the first to explore and describe the cell cycle in eukaryotic cells. 4 Using radioactive tracers (the technique which revolutionized the field of cell biology), they found that, unlike bacteria, eucariotic cells synthesized DNA only in S-phase rather than continually through interphase. The definition of cell phases: G1, S, G2 and mitosis formed the basis for the modern studies of growth control in molecular biology and biomedicine.…”

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confidence: 99%

The contribution of women to radiobiology: Marie Curie and beyond

Gasińska

2016

Reports of Practical Oncology & Radiotherapy

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Radiobiology Ionizing radiationTumor radiobiology a b s t r a c t Marie Sklodowska-Curie, an extraordinary woman, a Polish scientist who lived and worked in France, led to the development of nuclear energy and the treatment of cancer. She was the laureate of two Nobel Prizes, the first woman in Europe who obtained the degree of Doctor of Science and opened the way for women to enter fields which had been previously reserved for men only. As a result of her determination and her love of freedom, she has become an icon for many female scientists active in radiation sciences. In 1911, Marie Sklodowska-Curie was awarded her second Nobel Prize for the discovery of radium. Her pioneering work demonstrated that radiation is a powerful tool with a wide range of potential applications, which include a range of diagnostic and therapeutic medical procedures. Marie Curie has left a great deal to the world. Her work led to the development http://dx

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Synthesis of Desoxyribonucleic Acid in Normal and Irradiated Cells and Its Relation to Chromosome Breakage

Cited by 133 publications

References 14 publications

[beta]-Tubulin Accumulation and DNA Replication in Imbibing Tomato Seeds

[beta]-Tubulin Accumulation and DNA Replication in Imbibing Tomato Seeds

The concept of the quiescent centre and how it found support from work with X-rays. I. Historical perspectives

The contribution of women to radiobiology: Marie Curie and beyond

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