Synthesis of gentamicin-grafted-chitosan with improved solubility and antibacterial activity

Yan, Tingting; Li, Chengpeng; Ouyang, Qianqian; Zhang, Dongying; Zhong, Qingkun; Li, Puwang; Li, Sidong; Yang, Ziming; Wang, Tao; Zhao, Qi

doi:10.1016/j.reactfunctpolym.2019.01.013

Cited by 32 publications

(19 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentration of all samples was 1 mg/mL. GT's graft rate was 22.1%, as reported in our previous research [31].…”

Section: In Vitro Antibacterial Assaysupporting

confidence: 74%

“…To fabricate CS-GT hydrogel, 8 g of PVP was firstly dissolved in 32 g of distilled water, which was then mixed with 20 g CS-GT aqueous solution containing 5 g of CS-GT [31] and 40 g glycerin under magnetic stirring till a homogeneous solution was obtained. GT hydrogel was prepared using the same method, where 5 g GT was introduced to replace the 5 g CS-GT.…”

Section: Preparation Of Cs-gt Hydrogelmentioning

confidence: 99%

“…CS-GT has been successfully prepared in a previous study [31]. In this study, in order to investigate its potential scald healing-promoting effect, the antimicrobial activity of CS-GT was further studied, and the cytocompatibility and hemocompatibility of CS-GT were determined by cell viability assay and hemolysis assay.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

et al. 2020

Self Cite

View full text Add to dashboard Cite

Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of CS-GT hydrogel was systematically explored in this article. It was found that cell viability shows a declined inclination with the prolonged culture time and the increased concentration of CS-GT. After three day’s culture, the cell viability could still remain at 79.72% when CS-GT concentration was as high as 1000 μg/mL. On the other hand, the hemolysis rate of CS-GT was lower than 5% when its concentration is 800 μg/mL. Therefore CS-GT has good cytocompatibility and hemocompatibility. A wound-healing experiment has shown that the skin healing rate of CS-GT hydrogel was the highest at 99.61%, followed by the positive control (wet burn ointment) 94.98%, GT hydrogel 87.50%, and matrix 77.39%. The blank control group, however, possessed the lowest healing rate of 75.45%. Further analysis indicated that CS-GT hydrogel could promote the synthesis of total protein (TP) in skin granulation tissue, resulting in the enhanced hydroxyproline (HYP) content, which facilitated collagen fibrogenesis, reduced cytokine expression in an inflammatory response, and, ultimately, accelerated wound healing. To sum up, CS-GT hydrogel is a promising scald dressing.

show abstract

“…The concentration of all samples was 1 mg/mL. GT's graft rate was 22.1%, as reported in our previous research [31].…”

Section: In Vitro Antibacterial Assaysupporting

confidence: 74%

Section: Preparation Of Cs-gt Hydrogelmentioning

confidence: 99%

See 1 more Smart Citation

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…In 2019, we successfully synthesized a chitosan–gentamicin conjugate (CS-GT) via oxidation and a Schiff base reaction. Compared to CS, its water-solubility and antimicrobial activity are significantly improved [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…Our previous research also found that CS-GT-based hydrogels, with antimicrobial activity, cytocompatibility, and hemocompatibility, can accelerate the wound healing of New Zealand rabbits [ 27 ]. Particularly, CS-GT can inhibit pathogenic Escherichia coli , Pseudomonas aeruginosa , and Staphylococcus aureus effectively [ 26 ]. Therefore, CS-GT may be potentially applied in aquatic farming.…”

Section: Introductionmentioning

confidence: 99%

Effects of Chitosan–Gentamicin Conjugate Supplement on Non-Specific Immunity, Aquaculture Water, Intestinal Histology and Microbiota of Pacific White Shrimp (Litopenaeus vannamei)

Liang

Hou

et al. 2020

Marine Drugs

Self Cite

View full text Add to dashboard Cite

When the aquaculture water environment deteriorates or the temperature rises, shrimp are susceptible to viral or bacterial infections, causing a large number of deaths. This study comprehensively evaluated the effects of the oral administration of a chitosan–gentamicin conjugate (CS-GT) after Litopenaeus vannamei were infected with Vibrio parahaemolyticus, through nonspecific immunity parameter detection, intestinal morphology observation, and the assessment of microbial flora diversification by 16S rRNA gene sequencing. The results showed that the oral administration of CS-GT significantly increased total hemocyte counts and reduced hemocyte apoptosis in shrimp (p < 0.05). The parameters (including superoxide dismutase, glutathione peroxidase, glutathione, lysozyme, acid phosphatase, alkaline phosphatase, and phenoloxidase) were significantly increased (p < 0.05). The integrity of the intestinal epithelial cells and basement membrane were enhanced, which correspondingly alleviated intestinal injury. In terms of the microbiome, the abundances of Vibrio (Gram-negative bacteria and food-borne pathogens) in the water and gut were significantly reduced. The canonical correspondence analysis (CCA) showed that the abundances of Vibrio both in the water and gut were negatively correlated with CS-GT dosage. In conclusion, the oral administration of CS-GT can improve the immunity of shrimp against pathogenic bacteria and significantly reduce the relative abundances of Vibrio in aquaculture water and the gut of Litopenaeus vannamei.

show abstract

Imparting Antimicrobial and Antifouling Properties to Anion Exchange Membrane through the Modification with Gentamicin‐Based Polymer

Luo

Yao

Liao

et al. 2021

Adv Materials Inter

View full text Add to dashboard Cite

A series of gentamicin‐modified anion exchange membranes (AEMs) immobilized by mussel‐inspired dopamine coating is prepared in this work. The purpose is to improve antibacterial activity and fouling resistance of the commercial AEMs (AEM Type I, Fujifilm Co.). As a result of the applied modification, the hydrophilicity of membrane surface improved significantly. Thus, the as‐prepared modified AEMs exhibit the good antifouling property, in electrodialysis (ED) experiments by using the sodium dodecylbenzenesulfonate and albumin from bovine serum as model foulants. Furthermore, taking into account the results of the antibacterial test, the resulting modified AEMs exhibit improved antibacterial property for Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). After a brief contact with bacteria, the antibacterial rates of modified AEMs coating with gentamicin‐based polymer are 80.11% for E. coli and 90.66% for S. aureus, which are much higher than pristine AEM (6.28% and 9.07%), respectively. In addition, the modified membrane has good operational stability during ED process and recovery ability after contamination, and also maintains satisfactory electrochemical properties. This investigation highlights a design of simple strategy for the preparation of antimicrobial and antifouling AEM, which can be used for ED.

show abstract

Synthesis of gentamicin-grafted-chitosan with improved solubility and antibacterial activity

Cited by 32 publications

References 47 publications

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

Effects of Chitosan–Gentamicin Conjugate Supplement on Non-Specific Immunity, Aquaculture Water, Intestinal Histology and Microbiota of Pacific White Shrimp (Litopenaeus vannamei)

Imparting Antimicrobial and Antifouling Properties to Anion Exchange Membrane through the Modification with Gentamicin‐Based Polymer

Contact Info

Product

Resources

About