Synthesis of Glass‐Coated SERS Nanoparticle Probes via SAMs with Terminal SiO<sub>2</sub> Precursors

Schütz, Max; Küstner, Bernd; Bauer, Manuel; Schmuck, Carsten; Schlücker, Sebastian

doi:10.1002/smll.200902065

Cited by 45 publications

(61 citation statements)

References 32 publications

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“…As shown in Figure 2, a DTNB Raman spectrum free of contaminating signals could be recorded from the nanostars functionalized with DTNB and SAM, with the highest intensity at 1,333 cm -1 , resulting in an spectrum that was identical to what had previously been described in the literature. 33,34,39,40 This confirms that the SAM molecules did not replace all the DTNB molecules on the nanostars. As expected for the nanostars-SAM, no SERS signal could be measured.…”

Section: Nanostar Synthesis and Functionalizationmentioning

confidence: 64%

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

D’Hollander¹,

Mathieu²,

Jans³

et al. 2016

IJN

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The need for sensitive imaging techniques to detect tumor cells is an important issue in cancer diagnosis and therapy. Surface-enhanced Raman scattering (SERS), realized by chemisorption of compounds suitable for Raman spectroscopy onto gold nanoparticles, is a new method for detecting a tumor. As a proof of concept, we studied the use of biocompatible gold nanostars as sensitive SERS contrast agents targeting an ovarian cancer cell line (SKOV3). Due to a high intracellular uptake of gold nanostars after 6 hours of exposure, they could be detected and located with SERS. Using these nanostars for passive targeting after systemic injection in a xenograft mouse model, a detectable signal was measured in the tumor and liver in vivo. These signals were confirmed by ex vivo SERS measurements and darkfield microscopy. In this study, we established SERS nanostars as a highly sensitive contrast agent for tumor detection, which opens the potential for their use as a theranostic agent against cancer.

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Section: Nanostar Synthesis and Functionalizationmentioning

confidence: 64%

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

D’Hollander¹,

Mathieu²,

Jans³

et al. 2016

IJN

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“…In general, they can involve one or more nanoparticles conjugated to (1) a highly polarizable Raman reporter molecule, (2) an affinity ligand such as an aptamer or antibody, and (3) a steric or electrostatic capping agent for stabilization in high salt environments [150,151]. Oligonucleotides, antibodies, protein antigens, small molecules, and dyes can all be immobilized onto metallic nanoparticles using thiol end groups, bifunctional PEG linkers, or sequential click chemistry [152][153][154][155]. Extensive work has been done by groups such as Graham et al who utilize various oligonucleotide and resonant dye-coated nanoprobes to form SERRS active nanoassembly complexes for multiplexed DNA detection [156].…”

Section: Molecularly Mediated Colloidal Sersmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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Point-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere -from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted "ASSURED" (Affordable, Sensitive, Specific, Userfriendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

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“…24,25,95,99,110,111 In this section, different encapsulation approaches for SERS labels will be discussed.…”

Section: Protection and Stabilizationmentioning

confidence: 99%

“…15 shows two different synthesis routes towards silica-encapsulated SERS labels comprising a full SAM. 24,95 In the rst route, depicted in Fig. 15, le, the addition of Raman labels (Ra) to AuNPs leads to SAMcoated AuNPs (1a).…”

Section: Silica-encapsulation Of Sers Labelsmentioning

confidence: 99%

“…15, right): both the Raman reporter molecule (Ra) and the terminal SiO 2 -precursor are covalently bound to each other (Ra-SiO 2 ), i.e., both functions are merged into a single molecular unit. 95 In this approach, APTMS (3-amino-n-propyltrimethoxysilane) was covalently conjugated to mercaptobenzoic acid derivatives as Raman reporters. The addition of TEOS, its subsequent hydrolysis and condensation then led to the formation of a silica shell since the SERS labels are already vitreophilic due to the terminal SiO 2 precursor.…”

Section: Silica-encapsulation Of Sers Labelsmentioning

confidence: 99%

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Rational design and synthesis of SERS labels

Wang

Schlücker

2013

Analyst

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143

147

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SERS labels are a new class of nanotags for optical detection based on Raman scattering. Central advantages include their spectral multiplexing capacity due to the small line width of vibrational Raman bands, quantification based on spectral intensities, high photostability, minimization of autofluorescence from biological specimens via red to near-infrared (NIR) excitation, and the need for only a single laser excitation line. Current concepts for the rational design and synthesis of SERS labels are summarized in this review. Chemical constituents of SERS labels are the plasmonically active metal colloids for signal enhancement upon resonant laser excitation, organic Raman reporter molecules for adsorption onto the metal surface for identification, and an optional protective shell. Different chemical approaches towards the synthesis of rationally designed SERS labels are highlighted, including also their subsequent bioconjugation.

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Synthesis of Glass‐Coated SERS Nanoparticle Probes via SAMs with Terminal SiO₂ Precursors

Cited by 45 publications

References 32 publications

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

Rational design and synthesis of SERS labels

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Synthesis of Glass‐Coated SERS Nanoparticle Probes via SAMs with Terminal SiO2 Precursors

Cited by 45 publications

References 32 publications

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

Rational design and synthesis of SERS labels

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Product

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Synthesis of Glass‐Coated SERS Nanoparticle Probes via SAMs with Terminal SiO₂ Precursors