Synthesis of glyceryl ferulate by immobilized ferulic acid esterase

Abstract

Glyceryl ferulate was synthesized by the condensation of ferulic acid with glycerol using Pectinase PL "Amano" from Aspergillus niger, which contained ferulic acid esterase, to improve the water-solubility of ferulic acid. The optimum reaction medium was glycerol/0.1 M acetate buffer, pH 4.0, (98:2 v/v). The enzyme immobilized onto Chitopearl BCW3003 exhibited the highest activity among the those immobilized onto various kinds of Chitopearl BCW resins. The optimum temperature for the immobilized enzyme was 50 degrees C, and it could be reused at least five times without a significant loss in activity for the synthesis of glyceryl ferulate in batch reaction. Storage of the reaction mixture at 25 degrees C improved the molar fraction of glyceryl ferulate relative to the dissolved ferulic residues.

Chemoenzymatic synthesis of feruloylated monoacyl- and diacyl-glycerols in ionic liquids

“…The glyceryl ferulate (1-glyceryl ferulate and 2-glyceryl ferulate) yield increased sharply until 3 h to reach 89%, and then increased smoothly until 10 h to reach 98% (Fig. 3), which is much higher than that obtained in all previously reported routes (Matsuo et al 2008;Sun et al 2007b;Tsuchiyama et al 2006). This good performance obtained in this study could be explained by the fact that ionic liquids are not volatile and Fig.…”

Kinetics of enzymatic synthesis of monoferuloyl glycerol and diferuloyl glycerol by transesterification in [BMIM]PF6

“…Feruloyl glycerols (FGs) are the natural derivatives of FA, which are widely used as UV absorbers and antioxidants in many commercial applications (such as, cosmetics, pharmaceuticals, and food industries) [5][6][7]. However, it is difficult to separate FGs from natural raw materials since the contents of monoferuloyl glycerol (MFG) and diferuloyl glycerol (DFG) are very low (<0.1%) [8], which made FGs synthesis very interesting [9][10][11][12].…”

High Efficient Synthesis of Enzymatic 2-Ethylhexyl Ferulate at Solvent-Free and Reduced Pressure Evaporation System

“…The esterification of FA with monosaccharides as well as n-octyl glycoside to produced hydrophilic feruloylated derivatives. The water-soluble derivative of glyceryl ferulate was biosynthesized by esterification of FA with glycerol using pectinase [17]. Solvent-free enzymatic transesterification of EF with triolein (TO) with a higher EF conversion 77% [18].…”