Synthesis of glycidyl methacrylate-based matrix and its application in affinity chromatography of urokinase

Tang, Meng; Cao, Xuejun; Liu, Zuozhen; Wu, Xingyan; Dai, Guohao

doi:10.1016/s0032-9592(99)00002-3

Cited by 12 publications

(7 citation statements)

References 9 publications

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“…Poly(glycidyl methacrylate) (PGMA) has attracted much attention for its more hydrophilic characteristics than polystyrene and the abundant surface functional epoxy groups, which can be directly coupled with biomolecules or further modified for a variety of biological applications. Spherical PGMA particles are typically produced in aqueous medium by suspension polymerization14, 15 or by multi‐step seeded polymerization 16, 17. Recently, dispersion copolymerization of glycidyl methacrylate (GMA) and other acrylic monomers have also been reported 18, 19.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of monodisperse nonporous crosslinked poly(glycidyl methacrylate) particles with metal affinity ligands for protein adsorption

Guan

Liu

2005

Polymer International

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Monodisperse nonporous crosslinked poly(glycidyl methacrylate) (PGMA) particles with immobilized metal affinity ligands were prepared for selective recovery of proteins. The PGMA particles, with an average size of 2.2 µm, were prepared by a simple dispersion polymerization of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA). The particles were characterized by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). The epoxy groups of the particles were modified with the metal chelating agent iminodiacetic acid (IDA), which forms metal-IDA chelates at the active sites. After charging with copper ions, the particles were used to recover a model protein, bovine hemoglobin (BHb), in a batchwise manner. The particles had the adsorption capacity of 218.7 mg g −1 with little nonspecific adsorption. The adsorption behavior could be described with the Langmuir equation. The effect of pH on the adsorption was also studied. Regeneration of the metal-chelated particles was easily performed with 50 mmol L −1 ethylenediaminetetraacetic acid (EDTA), followed by washing with water and reloading with Cu 2+ . The particles could be very useful as an affinity separation adsorbent.

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Section: Introductionmentioning

confidence: 99%

Synthesis of monodisperse nonporous crosslinked poly(glycidyl methacrylate) particles with metal affinity ligands for protein adsorption

Guan

Liu

2005

Polymer International

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“…Due to these features, polymer microspheres have found use in various ÿelds, such as the traditional industries of paint, ink, and paper, as well as ÿne applications in recent years, e.g. carriers for enzymes (Hayashi and Ikada, 1990;Omi et al, 1997;Tang et al, 1999;Brahim et al, 2002), separation media for proteins (Ugelstad et al, 1992;Okubo and Hatorri, 1993;Inomata et al, 1995;Feng et al, 1998;Li et al, 2000), DNAs (Uhlen, 1989;Kawaguchi et al, 1989) and cells (Ugelstad et al, 1993), carriers for protein refolding (Shimizu et al, • Immunoassay microspheres (Dolitzky et al, 1994), on which antibodies or antigens are immobilized, are used to detect or separate their complementary antigens or antibodies. These microspheres should be spherical with smooth surfaces and small enough to give large speciÿc areas.…”

Section: Introductionmentioning

confidence: 99%

Compromise between dominant polymerization mechanisms in preparation of polymer microspheres

2004

Chemical Engineering Science

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“…Monodispersed micron‐sized polymer particles have various applications, e.g . support materials for enzymes, and column packing for affinity chromatography . The particle size distribution in suspension polymerization in stirred tanks is normally governed by droplet break‐up and coalescence during the dispersion of the monomer(s) in water and during the first part of the polymerization.…”

Section: Introductionmentioning

confidence: 97%

Micron‐Sized Polymer Particles by Membrane Emulsification

Simons

Keurentjes

Meuldijk

2013

Macromolecular Symposia

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Summary Dispersions of micron‐sized styrene droplets in water were produced by straight through microchannel arrays. The monomer droplets have been polymerized by suspension polymerization using an initiator system, which was only oil soluble. A significant amount of submicron particles was formed by secondary nucleation. The side reaction was emulsion polymerization, which was confirmed by the molecular weight distribution of the reaction product. Suppression of secondary nucleation was only partially possible by using the water soluble inhibitor NaNO2. Increasing the monomer to water ratio before polymerization resulted in less emulsion polymerization. Molecular weight was found to be strongly dependent on the initiator concentration as well as on the inhibitor concentration. It was demonstrated that polymer particles with a diameter of about 10 μm can be produced using emulsification of the monomer by microchannel arrays prior to polymerization.

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Synthesis of glycidyl methacrylate-based matrix and its application in affinity chromatography of urokinase

Cited by 12 publications

References 9 publications

Synthesis of monodisperse nonporous crosslinked poly(glycidyl methacrylate) particles with metal affinity ligands for protein adsorption

Synthesis of monodisperse nonporous crosslinked poly(glycidyl methacrylate) particles with metal affinity ligands for protein adsorption

Compromise between dominant polymerization mechanisms in preparation of polymer microspheres

Micron‐Sized Polymer Particles by Membrane Emulsification

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