Synthesis of hyaluronan by normal and wounded rabbit iris

Koralewska-Makár, Anna; Sommarin, Yngve; Bruun, Anitha; Molander, Nils; Stenevi, Ulf; Ehinger, Berndt

doi:10.1007/pl00007894

Cited by 3 publications

(5 citation statements)

References 17 publications

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“…When cultured chick embryo fibroblasts are appropriately stimulated, extracellular hyaluronan reaches a 1.9‐fold accumulation in 6 hours and a 3.9‐fold accumulation in 10 hours (Moscatelli & Rubin 1977). Besides, in another study, hyaluronan synthesis has in iris been demonstrated both in vivo and in organ culture in normal and traumatized eyes (Koralewska‐Makár et al 2000). Thus the iris seems to be the site for hyaluronan production after laser irradiation and probably the most important source of the increased aqueous hyaluronan induced by the laser treatment.…”

Section: Discussionmentioning

confidence: 98%

“…We have previously shown that hyaluronan concentrations increase in iris tissue and in aqueous humor after surgical trauma (Koralewska‐Makár et al 2000, 1998). However, we previously used an iridectomy as our standard iris trauma, and then inevitably also induced corneal trauma in the form of a surgical incision and a suture.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that ocular hyaluronan is synthesized both in vivo and in vitro in the normal iris and wounded cornea and iris (Koralewska‐Makár et al 2000; Molander 1994). Trauma appears to activate this process.…”

mentioning

confidence: 99%

“…It also promotes corneal epithelial healing as well as corneal wound healing (Chung et al 1989). Endogenous hyaluronan is involved in corneal and iris response to surgical trauma (Koralewska‐Makár et al 2000, 1998; Molander et al 1993a, 1993b; Hassell et al 1983).…”

mentioning

confidence: 99%

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Influence of laser irradiation on endogenous hyaluronan in rabbit iris and aqueous humor

Koralewska-Makár

Johnsson

Bruun

et al. 2001

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To monitor changes of endogenous hyaluronan in the iris tissue and aqueous humor after an isolated trauma to the iris by argon laser irradiation of the anterior surface of the iris. Methods: Iris and aqueous hyaluronan concentrations in rabbit were measured with a radiometric assay at different time points after laser irradiation. Results: Total hyaluronan content in iris tissue increased 3-fold to a peak concentration of 71-72 mg/g at 1 and 2 days after laser treatment. Aqueous hyaluronan increased to a maximum of about 1.6 mg/ml at 2 h and 12 h after laser irradiation of the iris. Conclusions:The iris tissue responds with increased hyluronan synthesis to an isolated iris argon laser irradiation and it seems to be the most important source of aqueous hyaluronan.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Influence of laser irradiation on endogenous hyaluronan in rabbit iris and aqueous humor

Koralewska-Makár

Johnsson

Bruun

et al. 2001

Acta Ophthalmologica Scandinavica

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“…The changes in aqueous hyaluronan concentration corresponded to similar changes in the iris. In another study, we were able to demonstrate in situ production of hyaluronan by normal and wounded iris tissue in vivo as well as in culture (Koralewska‐Makár et al. 2000).…”

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confidence: 97%

Effects of sympathetic denervation on the hyaluronan content of the anterior segment in the normal and traumatized rabbit eye

Koralewska-Makár

Johnsson

Bruun

et al. 2002

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To determine whether there is any involvement of sympathetic nerves in the regulation of ocular hyaluronan production in the normal and traumatized rabbit iris. Methods: Unilateral sympathetic denervation was performed by removing the right superior cervical ganglion. Hyaluronan concentrations in the iris and aqueous were measured with a radiometric assay at various time intervals after denervation. Peripheral iridectomy was also performed in both denervated and non-denervated eyes. Results: Hyaluronan concentrations in the iris tissue after denervation were observed to have increased after 1 day, reaching a peak of 129.6 ∫ 5.7 mg/g wet weight at day 3. Two weeks later, hyaluronan concentrations had fallen back to normal levels. Ocular trauma with peripheral iridectomy in denervated eyes caused an increase of hyaluronan content of up to 253.5 ∫ 30.5 mg/g wet weight, which was not significantly different from hyaluronan concentrations observed after the same trauma in non-denervated eyes. Conclusion: Cervical sympathetic denervation results in a moderate increase of the hyaluronan content in the rabbit iris and does not appear to influence the hyaluronan response of the iris to trauma.

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Hyaluronan and Associated Proteins in the Visual System

Zako

Yoneda²

2004

Chemistry and Biology of Hyaluronan

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Synthesis of hyaluronan by normal and wounded rabbit iris

Abstract: Our findings demonstrate synthesis of hyaluronan by normal and traumatized iris. The iris seems to be the most important source of aqueous hyaluronan.

Cited by 3 publications

References 17 publications

Influence of laser irradiation on endogenous hyaluronan in rabbit iris and aqueous humor

Influence of laser irradiation on endogenous hyaluronan in rabbit iris and aqueous humor

Effects of sympathetic denervation on the hyaluronan content of the anterior segment in the normal and traumatized rabbit eye

Hyaluronan and Associated Proteins in the Visual System

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