Synthesis of Hydroxyapatite Nanoparticles with Tailorable Morphologies and Carbonate Substitutions Using a Wet Precipitation Method

Peng, Fei; Veilleux, Eric; Schmidt, Megan; Wei, Mei

doi:10.1166/jnn.2012.5714

Cited by 8 publications

(8 citation statements)

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“…When the OH – or PO 4 3– sites become occupied by carbonate ions, A- or B-type carbonated hydroxyapatites, respectively, can be achieved . Replacing the linear OH – or tetrahedral PO 4 3– ions with planar CO 3 2– ions causes changes in unit cell parameters (expansion or contraction of a or c -axis) . Biological apatites, e.g., bone mineral, usually belong to the so-called AB-type mineral, meaning that simultaneous carbonate substitution of OH – and PO 4 3– sites occurs, while greater changes in lattice parameters can point to a higher carbonate substitution degree in the hydroxyapatite structure .…”

Section: Results and Discussionmentioning

confidence: 99%

Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate

et al. 2020

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The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL–1) and HAP/CS/Gent (4.039 U mL–1) coatings, compared to the control (0.900 U mL–1)). Drug-release kinetics was investigated in deionized water at 37 °C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial “burst-release effect” (∼21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability >85%), providing a promising basis for further medical application trials.

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Section: Results and Discussionmentioning

confidence: 99%

Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate

et al. 2020

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“…[4][5][6][7][8] Hydroxyapatite [HAp; Ca 10 (PO 4 ) 6 (OH) 2 ] and its F-substituted counterparts (FHAp) are principle inorganic constituents of animal bones and teeth. [11][12][13][14][15][16][17][18][19] Among the various investigated silver-containing hybrid materials, HAp and FHAp supported Ag NPs composites have been of particular interest. [11][12][13][14][15][16][17][18][19] Among the various investigated silver-containing hybrid materials, HAp and FHAp supported Ag NPs composites have been of particular interest.…”

Section: Introductionmentioning

confidence: 99%

“…9,10 Articially synthesized HAp and FHAp are widely used in biomedical elds, catalyst, sensors, uorescencematerials, chromatography, and environmental phosphorus recovery. [11][12][13][14][15][16][17][18][19] Among the various investigated silver-containing hybrid materials, HAp and FHAp supported Ag NPs composites have been of particular interest. 20,21 Recent studies by Kaneda et al have revealed that HAp-supported Ag NPs exhibit high catalytic activity for the selective oxidation of various phenylsilanes into phenylsilanols in water 22 and selective hydration of nitriles to amides in aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Controllable synthesis of fluorapatite microcrystals decorated with silver nanoparticles and their optical properties

Jiang

Xie

2015

RSC Adv.

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An EDTA-assisted hydrothermal method is developed for the controlled preparation of fluorapatite microcrystals (FHAp MPs) decorated with silver nanoparticles (Ag NPs). The synthesis process involves the use of EDTA as both a chelating agent and a reducing agent, allowing the one-pot formation of Ag/FHAp composites. The resultant product was characterized by X-ray diffraction patterns (XRD), field emission scanning electron micrographs (FESEM), transmission electron microscopy (TEM), and UV-vis diffuse reflection spectroscopy (UV-DRS). The Ag NPs prepared via this method exhibit a monodispersed size distribution and essentially uniform dispersion on FHAp MPs supports. It was demonstrated that the morphology of FHAp MPs can be tuned by varying the concentration of silver nitrate in the starting reaction solution, or by varying the pH value of the reaction solution. A possible growth mechanism was discussed for the formation of Ag/FHAp composites. Additionally we also demonstrated that the resulting Ag/FHAp composites with different Ag loadings possess tunable optical properties.

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“…A suspension of HA was added to formulations 3–6. Briefly, HA was prepared via a metathesis reaction . HA powder was then added to 5 mL of a 1% (w/v) PVA (Fisher Scientific) solution and mixed on a stirring plate for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, HA was prepared via a metathesis reaction. 31,32 HA powder was then added to 5 mL of a 1% (w/v) PVA (Fisher Scientific) solution and mixed on a stirring plate for 15 min. The HA-PVA suspension was then added to the alginate-phosphate suspension and mixed until homogeneous.…”

Section: Preparation Of Hydrogel Formulationsmentioning

confidence: 99%

Development of a novel alginate‐polyvinyl alcohol‐hydroxyapatite hydrogel for 3D bioprinting bone tissue engineered scaffolds

Bendtsen

Quinnell

Wei

2017

J Biomedical Materials Res

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Three-dimensional printed biomaterials used as personalized tissue substitutes have the ability to promote and enhance regeneration in areas of defected tissue. The challenge with 3D printing for bone tissue engineering remains the selection of a material with optimal rheological properties for printing in addition to biocompatibility and capacity for uniform cell incorporation. Hydrogel biomaterials may provide sufficient printability to allow cell encapsulation and bioprinting of scaffolds with uniform cell distribution. In this study, a novel alginate-polyvinyl alcohol (PVA)-hydroxyapatite (HA) hydrogel formulation with optimal rheological properties for 3D bioprinting of mouse calvaria 3T3-E1 (MC3T3) cells into scaffolds of high shape fidelity has been developed. A systematic investigation was conducted to determine the effect of varying concentrations of alginate, phosphate, calcium, and the PVA-HA suspension in the formulation on the resulting viscosity and thus printability of the hydrogel. HA, the main mineral component in natural bone, was incorporated into the hydrogel formulation to create a favorable bone-forming environment due to its excellent osteoconductivity. Degradation studies in α-MEM cell culture media showed that the 3D printed alginate-PVA-HA scaffolds remained in-tact for 14 days. MC3T3 cells were well distributed and encapsulated throughout the optimal hydrogel formulation and expressed high viability through the completion of the 3D printing process. Thus, the development of this novel, osteoconductive, biodegradable, alginate-PVA-HA formulation and its ability to 3D bioprint tissue engineered scaffolds make it a promising candidate for treating personalized bone defects. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1457-1468, 2017.

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Synthesis of Hydroxyapatite Nanoparticles with Tailorable Morphologies and Carbonate Substitutions Using a Wet Precipitation Method

Cited by 8 publications

References 0 publications

Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate

Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate

Controllable synthesis of fluorapatite microcrystals decorated with silver nanoparticles and their optical properties

Development of a novel alginate‐polyvinyl alcohol‐hydroxyapatite hydrogel for 3D bioprinting bone tissue engineered scaffolds

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