Synthesis of magnetic gold mesoporous silica nanoparticles core shell for cellulase enzyme immobilization: Improvement of enzymatic activity and thermal stability

Poorakbar, Elahe; Shafiee, Abbas; Saboury, Ali Akbar; Rad, Behzad; Khoshnevisan, Kamyar; Ma’mani, Leila; Derakhshankhah, Hossein; Ganjali, Mohammad Reza; Hosseini, Morteza

doi:10.1016/j.procbio.2018.05.012

Cited by 127 publications

(34 citation statements)

References 55 publications

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“…These solid materials are developed by replacing the solvent trapped in the gel structure with a gas (e.g., air), leading to an open-ended mesoporous structure typically composed of 95–99% empty space [ 103 ]. Polysaccharide-based aerogels obtained with polymers with natural resources (alginate, agar, starch, cellulose, chitosan, chitin or pectin) are sustainable materials that can substitute silica aerogels in biomedical and pharmaceutical applications [ 104 , 105 , 106 ]. Different medical applications, such as tissue engineering, drug delivery or biosensing have been developed due to the advancement in bioaerologels research.…”

Section: Applications Of Alginate In the Medical Fieldmentioning

confidence: 99%

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

et al. 2020

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Initially used extensively as an additive and ingredient in the food industry, alginate has become an important compound for a wide range of industries and applications, such as the medical, pharmaceutical and cosmetics sectors. In the food industry, alginate has been used to coat fruits and vegetables, as a microbial and viral protection product, and as a gelling, thickening, stabilizing or emulsifying agent. Its biocompatibility, biodegradability, nontoxicity and the possibility of it being used in quantum satis doses prompted scientists to explore new properties for alginate usage. Thus, the use of alginate has been expanded so as to be directed towards the pharmaceutical and biomedical industries, where studies have shown that it can be used successfully as biomaterial for wound, hydrogel, and aerogel dressings, among others. Furthermore, the ability to encapsulate natural substances has led to the possibility of using alginate as a drug coating and drug delivery agent, including the encapsulation of probiotics. This is important considering the fact that, until recently, encapsulation and coating agents used in the pharmaceutical industry were limited to the use of lactose, a potentially allergenic agent or gelatin. Obtained at a relatively low cost from marine brown algae, this hydrocolloid can also be used as a potential tool in the management of diabetes, not only as an insulin delivery agent but also due to its ability to improve insulin resistance, attenuate chronic inflammation and decrease oxidative stress. In addition, alginate has been recognized as a potential weight loss treatment, as alginate supplementation has been used as an adjunct treatment to energy restriction, to enhance satiety and improve weight loss in obese individuals. Thus, alginate holds the promise of an effective product used in the food industry as well as in the management of metabolic disorders such as diabetes and obesity. This review highlights recent research advances on the characteristics of alginate and brings to the forefront the beneficial aspects of using alginate, from the food industry to the biomedical field.

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Section: Applications Of Alginate In the Medical Fieldmentioning

confidence: 99%

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

et al. 2020

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“…Thermal and chemical stabilities were considerably augmented in a wide range of pH values and temperatures. Vibrating-sample magnetometer (VSM) study results showed that the magnetic behavior of MNPs was not altered following the coating process [72]. Core-shell magnetic gold mesoporous silica was exploited as a support for cellulase immobilization (Scheme 7).…”

Section: Cellulase Immobilization On Composite-functionalized Mnpsmentioning

confidence: 99%

“…Providing unique physical and electronic properties and also providing a large surface area for biomolecules to anchor [69][70][71][72] Amino-based surface functionalization Novel strategies to enhance the enzyme's thermal and chemical stability [63][64][65][66][67] Chitosan-based surface functionalization…”

Section: Silica-based Surface Functionalizationmentioning

confidence: 99%

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Recent Advances of Cellulase Immobilization onto Magnetic Nanoparticles: An Update Review

et al. 2019

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Cellulosic enzymes, including cellulase, play an important role in biotechnological processes in the fields of food, cosmetics, detergents, pulp, paper, and related industries. Low thermal and storage stability of cellulase, presence of impurities, enzyme leakage, and reusability pose great challenges in all these processes. These challenges can be overcome via enzyme immobilization methods. In recent years, cellulase immobilization onto nanomaterials became the focus of research attention owing to the surface features of these materials. However, the application of these nanomaterials is limited due to the efficacy of their recovery process. The application of magnetic nanoparticles (MNPs) was suggested as a solution to this problem since they can be easily removed from the reaction mixture by applying an external magnet. Recently, MNPs were extensively employed for enzyme immobilization owing to their low toxicity and various practical advantages. In the present review, recent advances in cellulase immobilization onto functionalized MNPs is summarized. Finally, we discuss enhanced enzyme reusability, activity, and stability, as well as improved enzyme recovery. Enzyme immobilization techniques offer promising potential for industrial applications.

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“…In the last decade, the use of nanoparticles in enzyme stabilization has attracted increased attention based on their very small size of particles and large surface area . Magnetic nanoparticles show a high binding capacity to conjugated enzymes and can be used for enzyme immobilization . The potential of magnetic nanoparticles as carriers for immobilized enzymes can be increased by the use of nanoparticles containing silica with an ordered structure, such as KCC‐1, on the surface of magnetic supports.…”

Section: Introductionmentioning

confidence: 99%

A Laccase Heterogeneous Magnetic Fibrous Silica‐Based Biocatalyst for Green and One‐Pot Cascade Synthesis of Chromene Derivatives

et al. 2019

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Green cascade approaches, which utilize sustainable and recyclable heterogeneous biocatalysts, can be used in the catalysis of multicomponent organic reactions to synthesize biologically important substances. Three magnetic nanoparticles, iron (II, III) oxide, cobalt ferrite, and nickel ferrite, were prepared by co‐precipitation and functionalized with fibrous silica (KCC‐1), and characterized by scanning electron microscopy (SEM), X‐ray diffraction (XRD), and vibrating sample magnetometry (VSM). Laccase immobilized on CoFe2O4‐KCC‐1 was used as a heterogeneous biocatalyst for green and one‐pot cascade synthesis of 2‐amino‐5‐oxo‐4‐aryl‐4H,5H‐pyrano[3,2‐c]chromene‐3‐carbonitriles. The reaction was performed in the presence of 2,2,6,6‐tetramethylpiperidine‐1‐oxyl (TEMPO) as a synthetic redox mediator. The optimum reaction conditions were found to be as immobilized laccase (100 mg, 95 U) and TEMPO (2 mol‐%) in a 100 mm sodium citrate buffer (pH 4.5), 40 °C, and incubation time 17 h. About 80 % of initial activity of the immobilized laccase was retained after 15 independent runs.

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Synthesis of magnetic gold mesoporous silica nanoparticles core shell for cellulase enzyme immobilization: Improvement of enzymatic activity and thermal stability

Cited by 127 publications

References 55 publications

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

Recent Advances of Cellulase Immobilization onto Magnetic Nanoparticles: An Update Review

A Laccase Heterogeneous Magnetic Fibrous Silica‐Based Biocatalyst for Green and One‐Pot Cascade Synthesis of Chromene Derivatives

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