Synthesis of magnetic nanoparticles with immobilized aminophenylboronic acid for selective capture of glycoproteins

Aminophenyl boronic acids can form reversible covalent ester interactions with cis-diol-containing molecules, serving as a selective tool for binding glycoproteins as antibody molecules that possess oligosaccharides in both the Fv and Fc regions. In this study, amino phenyl boronic acid (APBA) magnetic particles (MPs) were applied for the magnetic separation of antibody molecules. Iron oxide MPs were firstly coated with dextran to avoid non-specific binding and then with 3-glycidyloxypropyl trimethoxysilane to allow further covalent coupling of APBA (APBA_MP MP (Q max). APBA_MPs were also tested for antibody purification directly from CHO cell supernatants. The particles were able to bind 98% of IgG loaded and to recover 95% of pure IgG (purity greater than 98%) at extremely mild conditions.

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“…The silica coating was carried out according to method described by Lin et al [18] with minor modifications. Briefly, 120 ml of aq.…”

Section: Silica and Tetraethoxy Silane Coating Of Iron Oxide Magneticmentioning

confidence: 99%

Boronic acid-modified magnetic materials for antibody purification

Dhadge

Hussain

Azevedo

et al. 2014

J. R. Soc. Interface.

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“…In addition, the moderate acidic elution condition make boronate affinity well coupled with mass spectrometry for analysis of cis-diol biomolecules in -omics [14][15][16]. Different types of boronate affinity materials have been developed for specific capture of glycoproteins [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions

Yang

Mao

et al. 2013

Anal Bioanal Chem

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In this study, a boronate-silica hybrid affinity monolith was prepared for specific capture of glycoproteins at neutral pH condition. The monolith was synthesized via a facile one-pot procedure in a stainless steel column by concurrently mixing hydrolyzed alkoxysilanes tetramethoxysilane and vinyltrimethoxysilane, organic monomer 3-acrylamidophenylboronic acid and initiator 2,2′-azobisisobutyronitrile together. The polycondensation of alkoxysilanes and copolymerization of organic monomer and vinyl-silica monolith were carried out successively by reacting at different temperatures. After optimizing the preparation conditions, the resulting hybrid affinity monolith was systematically characterized and exhibited excellent affinity to both cis-diol-containing small molecules and glycoproteins at neutral and physiological pH, including adenosine, horseradish peroxidase, transferrin and ovalbumin. The binding capacity of ovalbumin on monolith was measured to be 2.5 mg g −1 at pH 7.0. Furthermore, the hybrid affinity monolith was applied to the separation of transferrin from bovine serum sample at a physiological condition. Good repeatability was obtained and the relative standard deviations of retention time were 1.15 and 4.77 % (n=5) for run-torun and column-to-column, respectively.

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“…Afterward, only OB reappeared in the elution lane (lane 3) and the selectivity of the MIP nanospheres was further proven. Compared with other separation methods of glycoprotein [42], the imprinted nanospheres had a more satisfied selectivity. The adsorption rate of the imprinted nanospheres was faster than that of tumor marker-responsive gels [43], which can be attributed to the formation of the specific binding sites on the surface of the nanospheres.…”

Section: Real Sample Analysismentioning

confidence: 99%

Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein

Gao

et al. 2013

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Synthesis of magnetic nanoparticles with immobilized aminophenylboronic acid for selective capture of glycoproteins

Cited by 128 publications

References 32 publications

Boronic acid-modified magnetic materials for antibody purification

Boronic acid-modified magnetic materials for antibody purification

Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions

Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein

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