Synthesis of [Mn(Salen)Cl] Complex Compound and Superoxide Dismutase Activity Determination through Non-Enzymatic Method

Abstract

Superoxide dismutase (SOD) is an Mn-containing enzyme that catalyzes the conversion of superoxide (O2•−) into O2 and H2O2. To develop a small, non-protein Mn-containing SOD mimic, an [Mn(salen)Cl] complex was synthesized from MnCl2.4H2O and H2salen. The product yielded 75-90% reddish dark brown crystals indicative of the presence of manganese(III) metal ions. An elemental analysis showed that the compound contained: C = 48.36%; H = 4.34%; and N = 7.13%. Infrared spectra of this compound showed peaks at: υ(OH)=3426 (medium), υ(C=N)=1634 (very high), υ(CO)=1292 (high). An ESI-MS analysis (m/z) showed the presence of ionic species from the complex including (salen)(H+) = 269.0 and [Mn(salen)]+ = 320.9. Conductivity measurements revealed that the compound is neutral with a molar conductivity of 0.17 mS. Its magnetic moment of 5.2 BM was obtained from a magnetic susceptibility balance measurement, showing that the compound is paramagnetic. The SOD activity of this compound was given by IC50 = 2.5 ± 0.3 µM in a non-enzymatic riboflavin photoreduction in vitro test.

Playing with [{Mo6Bri6Sei2}(CN)a6]4− Cluster Building Blocks and Mn(salen)+ Nodes for the Crystal Engineering of Inorganic Coordination Polymers

“…The starting cluster compound Cs 2 K 2 [{Mo 6 Br i 6 Se i 2 }(CN) 6 ] and the manganese salen were prepared as reported previously. [10,16,17] Energy dispersive spectroscopy (EDS) was performed on Field emission Scanning Electron Microscope TM-3000 with Bruker Nano EDS analyzer. FT-IR spectra in KBr pellets were recorded on a Bruker Scimitar FTS 2000 in the range 4000-375 cm À 1 .…”

“…All chemicals and solvents were reagent grade and were used without further purifications. The starting cluster compound Cs 2 K 2 [{Mo 6 Br i 6 Se i 2 }(CN) 6 ] and the manganese salen were prepared as reported previously [10,16,17] . Energy dispersive spectroscopy (EDS) was performed on Field emission Scanning Electron Microscope TM‐3000 with Bruker Nano EDS analyzer.…”

A Multipurpose Metallophore and Its Copper Complexes with Diverse Catalytic Antioxidant Properties to Deal with Metal and Oxidative Stress Disorders: A Combined Experimental, Theoretical, and In Vitro Study

“…On the contrary, CAT, which is abundant in the peroxisomes, breaks down the H 2 O 2 into water and molecular O 2 . , It is known that the levels of SODs decrease with age, resulting in the increase in the level of ROS . One of the common strategies for dealing with this situation is the consumption of nutraceutical ingredients such as vitamins (A, C, and E) and flavonoids, because they can act as potent antioxidants and metal chelators. − Furthermore, they have long been recognized to possess anti-inflammatory, antiallergic, hepatoprotective, antithrombotic, antiviral, and anticarcinogenic activities, showing a direct relation between these diseases and ROS. , A more recent approach is the development of synthetic mimics of antioxidant metalloenzymes such as SOD and CAT. − Their examples include coordination compounds synthesized with porphyrins, H 2 salen-type ligands, macrocycles, corroles, and polydentate amines. − Recently, Policar and co-workers reviewed strategies for the design of efficient SOD mimics and their basic requirements like cellular uptake, speciation, catalytic activities, and distribution in cells …”