Synthesis of poly(2-methacryloyloxyethyl phosphorylcholine)-conjugated lipids and their characterization and surface properties of modified liposomes for protein interactions

Abstract

Polyethylene glycol (PEG) is frequently used for liposome surface modification. However, as PEGylated liposomes are cleared rapidly from circulation upon repeated injections, substitutes of PEG are being sought. We focused...

Effect of liposome surface modification with water-soluble phospholipid polymer chain-conjugated lipids on interaction with human plasma proteins

“…We have previously demonstrated the long-term stability of 1 mol% PMPC–liposomes, which were stable over 98 days. 22 This indicates that the surface coverage and molecular weight of PMPC are important for obtaining the optimal shielding effect from protein adsorption in human blood. The zeta potential of all analyzed liposomes became more negative after plasma incubation (Fig.…”

“…PMPC-lipids with polymerization degrees of 10, 20, 50, and 100 (3.7, 6.7, 16, and 30 kDa respectively) were synthesized based on the procedure reported by Adler et al 22 (Scheme 1). These lipids are hereinafter referred to as MPC10-lipid, MPC20-lipid, MPC50-lipid, and MPC100-lipid, respectively.…”

“…In addition, the PMPC-lipid-modified surface was not recognized by the anti-PEG antibody. 22 In this study, we modified liposomes with PMPC-lipids having different molecular weights of PMPC (from 3 to 30 kDa) and different molar ratios of PMPC-lipids (1, 5, and 10 mol%). Subsequently, we investigated the interaction between human plasma proteins and the modified liposomes to evaluate the protein profile and its relationship with the length of the PMPCchain, mol% of the PMPC-lipid, and protein adsorption, with emphasis on the binding of C3 to analyze the complement activation.…”

Cell-membrane-inspired polymers for constructing biointerfaces with efficient molecular recognition

“…107 To stabilize the liposomal structure, surface modification on the outer surface with some water-soluble polymers, such as PEO or poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) (PMPC) has been carried out. [108][109][110] Interestingly, the structural concept of lipid microspheres has been applied to an mRNA vaccine against SARS-CoV-2 virus. 111,112 4.1.3 Biointerfaces with natural phosphatidylcholine derivatives.…”

Emerging Phospholipid Nanobiomaterials for Biomedical Applications to Lab-on-a-Chip, Drug Delivery, and Cellular Engineering

“…Atom transfer radical polymerization (ATRP) is another kind of reversible-deactivation radical polymerization in which a carbon−carbon bond with a metal transition catalyst is formed. 44 In this strategy, reversible chain-end-capping by halogen atoms is used to guarantee that the momentary concentration of the polymer radical is less than the conventional technique. The termination ends with the elongation suppression and results in limited molecular weight dispensation.…”