Synthesis of Polymer Assembled Mesoporous CaCO₃ Nanoparticles for Molecular Targeting and pH-Responsive Controlled Drug Release

Abstract

CaCO3 nanoparticles are very suitable as intelligent carriers because of their ideal biocompatibility and biodegradability, especially their sensitivity to pH. In this paper, we use mesoporous CaCO3 nanoparticles as intelligent carrier, sodium alginate, and chitosan as alternating assembly materials, folic acid as target molecules, and exploit layer-by-layer assembly technology to achieve sensitive molecular targeting and pH response drug release. Mesoporous CaCO3 hybrid nanoparticles have high drug loading on doxorubicin. The effects of different pH values on drug release in vitro were studied by regulating simulated body fluids with different pH values. The cytotoxicity, targeting effect, and drug release of human cervical cancer cell line (HeLa) were studied by cell vitality and imaging experiments. All the evidence suggests that the smart mesoporous CaCO3 nanoparticles may be a potential clinical application platform for cancer therapy.

Magnetic Colloidal Particles in Combinatorial Thin-Film Gradients for Magnetic Resonance Imaging and Hyperthermia

“…Furthermore, the employment of magnetic polymer colloids to design multifunctional biothin films by incorporating various materials to obtain molecular-level control presents numerous biomedical applications. The layer-bylayer (LBL) self-assembled multilayer (SAMu) biothin films are not only useful for diagnostics but are also helpful in directing therapeutic agents by focusing on an external magnetic field to the target zone [11]. A broad range of stimuliresponsive thin films have been fabricated and are used as a point-of-care diagnostic devices that can be routinely applied in everyday clinical practice.…”

Hard, Soft, and Hard-and-Soft Drug Delivery Carriers Based on CaCO3 and Alginate Biomaterials: Synthesis, Properties, Pharmaceutical Applications

“…In addition, these carriers could provide cell targeting by such molecules as folic acid (FA) [ 115 ], peptide (KALA) [ 116 ], therapeutic phosphorylated EEEEpYFELV (EV) peptide [ 117 ], PEG [ 118 ], hyaluronate/glutamate [ 119 ]. For example, Xing et al [ 115 ] used chitosan and sodium alginate (SA) as coatings and modified the surface with PEG and targeting molecules FA to prepare multilayer DOX/CCPs carriers via the co-precipitation method combined with the LbL technology ( Figure 6 ).…”

“…In addition, these carriers could provide cell targeting by such molecules as folic acid (FA) [ 115 ], peptide (KALA) [ 116 ], therapeutic phosphorylated EEEEpYFELV (EV) peptide [ 117 ], PEG [ 118 ], hyaluronate/glutamate [ 119 ]. For example, Xing et al [ 115 ] used chitosan and sodium alginate (SA) as coatings and modified the surface with PEG and targeting molecules FA to prepare multilayer DOX/CCPs carriers via the co-precipitation method combined with the LbL technology ( Figure 6 ). In vitro experiments illustrated that DOX release rate of the hybrid system was slow, where only 35% of the total encapsulated amount of loaded drugs was released after 150 h of incubation, indicating that the system had long-term drug release characteristics caused by extensive electrostatic interactions between the polymer electrolyte and drug.…”

“…Interestingly, the combination of these two excellent biomaterials has also been extensively studied ( Figure 10 ). As mentioned above, the polyelectrolyte layer on the surface of CCPs can effectively improve the drug encapsulation and delivery efficiency, thereby achieving higher drug efficacy [ 115 , 281 ] ( Figure 10 A).…”

Recent Trends in Nanocarrier-Based Targeted Chemotherapy: Selective Delivery of Anticancer Drugs for Effective Lung, Colon, Cervical, and Breast Cancer Treatment

“…The intelligent NPs were constructed with chitosan and sodium alginate as alternative materials, folic acid as cancer cell targeting ligand, and layer-by-layer as pH-responsive approach. Cervical cancer cell-targeted delivery of doxorubicin was achieved in a controlled manner via pH responsiveness and receptor recognition [122]. Another recent study reported a novel bioinspired NP strategy for simultaneous delivery of paclitaxel and siRNA for effective treatment of cervical malignancies.…”