Synthesis of polymer-functionalized nanoscale graphene oxide with different surface charge and its cellular uptake, biosafety and immune responses in Raw264.7 macrophages

Wang, Bing; Su, Xiaopeng; Liang, Junlong; Yang, Lifeng; Hu, Qinli; Shan, Xinyi; Wan, Junmin; Hu, Zhiwen

doi:10.1016/j.msec.2018.04.096

Cited by 59 publications

(42 citation statements)

References 39 publications

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“…Many studies on the biocompatibility of GRMs have been reported mainly on the mouse macrophage cell line RAW 264.7. Wang et al observed that RAW 264.7 cells incubated with GO functionalized with polyethylene glycol (PEG) and polyethylenimine (PEI) were 90% viable at low concentrations and that GO-PEG could trigger an inflammatory response eliciting IL-6 secretion [8]. In another study, rGO (reduced GO) microfibers inhibited cell proliferation without affecting the viability of macrophages.…”

Section: Introductionmentioning

confidence: 99%

Few Layer Graphene Does Not Affect Cellular Homeostasis of Mouse Macrophages

et al. 2020

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Graphene-related materials (GRMs) are widely used in various applications due to their unique properties. A growing number of reports describe the impact of different carbon nanomaterials, including graphene oxide (GO), reduced GO (rGO), and carbon nanotubes (CNT), on immune cells, but there is still a very limited number of studies on graphene. In this work, we investigated the biological responses of few layer graphene (FLG) on mouse macrophages (bone marrow derived macrophages, BMDMs), which are part of the first line of defense in innate immunity. In particular, our paper describes our findings of short-term FLG treatment in BMDMs with a focus on observing material internalization and changes in general cell morphology. Subsequent investigation of cytotoxicity parameters showed that increasing doses of FLG did not hamper the viability of cells and did not trigger inflammatory responses. Basal level induced autophagic activity sufficed to maintain the cellular homeostasis of FLG treated cells. Our results shed light on the impact of FLG on primary macrophages and show that FLG does not elicit immunological responses leading to cell death.

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Section: Introductionmentioning

confidence: 99%

Few Layer Graphene Does Not Affect Cellular Homeostasis of Mouse Macrophages

et al. 2020

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“…Macrophages, an important part of innate immune system, are related to various diseases and play a vital role in sepsis by regulating immune responses and inflammatory factors secretion, such as tumor necrosis factor (TNF)-α, IL-10, and IFN-g [3,4]. Recent research has indicated that excessive production of inflammatory cytokines was observed during sepsis [5,6].…”

Section: Introductionmentioning

confidence: 99%

Expression and role of ABIN1 in sepsis: In vitro and in vivo studies

Sun

Meng

et al. 2020

Open Medicine

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In this research, we attempted to explain the effect and the related molecular mechanisms of ABIN1 in lipopolysaccharide (LPS)-induced septic mice or RAW264.7 macrophages. LPS was adopted to treat RAW264.7 macrophages for 4 h, and the levels of inflammatory factors were assessed by ELISA. Besides, ABIN1 expression was measured by quantitative reverse transcription polymerase chain reaction. Apparently, LPS enhanced immunoreaction, suggested by increased expression of IL-1β, tumor necrosis factor (TNF)-α, and IL-6. ABIN1 levels were obviously reduced compared to the control. Furthermore, we evaluated the roles of ABIN1-plasmid in immunoreaction and nuclear factor-κB (NF-κB) pathway. We found that ABIN1-plasmid significantly reduced the expression of IL-1β, TNF-α, and IL-6 in LPS-treated cells and inhibited NF-κB pathway activation. Meanwhile, a septic mouse mode was conducted to validate the role of ABIN1 in inflammatory response and organ damage in vivo. These data suggested that ABIN1-plasmid significantly inhibited the secretion of inflammatory cytokines and Cr, BUN, AST, and ALT levels in the serum of LPS-stimulated mice compared to LPS + control-plasmid group, reflecting the relieved inflammation and organ injury. In summary, the present findings indicated that ABIN1 alleviated sepsis by repressing inflammatory response through NF-κB signaling pathway, emphasizing the potential value of ABIN1 as therapeutic strategy for sepsis.

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“…This result is in agreement with the literature suggesting that internalised GO ends up in the lysosomes 9,32 . Some studies show that surface charge of GO can be modified using different chemical moieties in order to influence the intracellular fate of GO 16,18,80 From Video S1-S6, we could also see a consistent exchange of both materials between the lysosomal vesicles, and with no obvious disruption to the lysosome membrane up to 48 h of treatment. These findings confirm results from our previous study, where we observed no toxicity or lysosomal rupture after treatment with s-GO using cell culture medium supplemented with 10% FBS 34 .…”

Section: Intracellular Fate Of Go We Interrogated Intracellular Fatementioning

confidence: 73%

Dynamic interactions and intracellular fate of label-free, thin graphene oxide sheets within mammalian cells: role of lateral sheet size

Chen

Crică

Rosano

et al. 2019

Preprint

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Graphene oxide (GO) holds great potential for biomedical applications, however fundamental understanding of the way it interacts with biological systems is still lacking even though it is a prerequisite for successful clinical translation. In this study, we exploited intrinsic fluorescent properties of GO to establish the relationship between lateral dimensions of the material, its cellular uptake mechanism and intracellular fate. Label-free GO with distinct lateral dimensions, small (s-GO) and ultra-small (us-GO), was synthesized and thoroughly characterised both in water and in biologically relevant cell culture medium. Interactions of the material with a range of non-phagocytic mammalian cell lines (BEAS-2B, NIH/3T3, HaCaT, 293T) were studied using a combination of complementary analytical techniques (confocal microscopy, flow cytometry and TEM). The uptake mechanism was interrogated using a range of pharmaceutical inhibitors for main endocytic pathways (ethyl-isopropyl amiloride, monodansylcadaverine, chlorpromazine, genistein, cytochalasin D, latrunculin A, dynasore and sodium azide), and validated using negatively charged polystyrene beads with different diameters (0.1 and 1 μm). Regardless of lateral dimension, both types of GO were found to interact with the plasma membrane and to be efficiently taken up by a panel of cell lines in a timeand dose-dependent manner. s-GO was internalised mainly via macropinocytosis while us-GO used mainly clathrin-and caveolae-mediated endocytosis. Lastly, we show that both s-GO and us-GO terminate in lysosomal compartments for up to 48 h. Our results aim to offer significant insight into the mechanism of interaction of GO with non-phagocytic cell lines that can be exploited for the design of biomedically applicable 2D transport systems.

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Synthesis of polymer-functionalized nanoscale graphene oxide with different surface charge and its cellular uptake, biosafety and immune responses in Raw264.7 macrophages

Cited by 59 publications

References 39 publications

Few Layer Graphene Does Not Affect Cellular Homeostasis of Mouse Macrophages

Few Layer Graphene Does Not Affect Cellular Homeostasis of Mouse Macrophages

Expression and role of ABIN1 in sepsis: In vitro and in vivo studies

Dynamic interactions and intracellular fate of label-free, thin graphene oxide sheets within mammalian cells: role of lateral sheet size

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