Synthesis of prednisolone molecularly imprinted polymer nanoparticles by precipitation polymerization

Abstract

The prednisolone molecularly imprinted polymer nanoparticles (prednisolone-MIPs) were prepared via precipitation polymerization. The synthetic conditions were performed by optimizing of functional monomers (acrylamide and methacrylic acid) and crosslinkers (ethylene glycol dimethacrylate and pentaerythritol triacrylate) with defined the synthetic ratio of template molecule:functional monomer:crosslinker as 1:7:30. The acquired MIPs were studied for their binding quantitation by kinetic adsorption analysis. Two of four synthetic conditions reached an equilibrium binding capacity at 960 minutes. In addition, the equilibrium adsorption, kinetic models, adsorption isotherms, and molecular selectivity were also evaluated in this study.The pseudo-second order was the best fitted kinetic model with good agreement on Langmuir isotherm of both optimum conditions. The molecular recognition of prednisolone-MIPs was further carried out to demonstrate the selectivity performance and found that prednisolone-MIPs had a good recognition to target prednisolone rather than structural analogue.

MOLECULARLY IMPRINTED POLYMER NANOPARTICLES (MIP-NPs) APPLICATIONS IN ELECTROCHEMICAL SENSORS

“…Alternative methods provide imprinted polymer in regular size and shape. The other methods, including precipitation polymerization [27,28], emulsion polymerization [29], iniferter polymerization [30], and core-shell particles [31]. Precipitation polymerization gives a better control of shape and size.…”

Recent Advances in Solid-Phase Extraction (SPE) Based on Molecularly Imprinted Polymers (MIPs) for Analysis of Hormones

“…The general scheme for precipitation polymerization in shown in Figure 3. In contrast to bulk polymerization, this form of polymerization necessitates a significant amount of solvent [74]. It should also be noted that many variables, such as the polarity of the solvent, reaction temperature, and stirring speed, affect the size of the particles obtained, so reaction conditions should be carefully regulated [75].…”

Sample Preparation Using Molecularly Imprinted Polymers

“…40 Boronic acid was also used for its ability to form a reversible covalent complex with cis-diol compounds such as luteolin, 48,49 vitamin B12, 50 sialic acid, 51 or for the specific trapping of glycans of glycoproteins as discussed in more detail below. Many works reported the screening of different synthesis conditions by preparing MIPs using different types of monomers, cross-linkers, template/monomer or monomer/cross-linker ratios, natures of the porogen, 28,30,39,43,[52][53][54][55][56][57][58][59][60][61][62][63][64][65] and even the pH when boronic acid is involved. 50,66 The evaluation of the resulting MIPs consists mainly in comparing the binding capacity of the MIP for the target analyte with the one obtained with the corresponding non-imprinted polymer (NIP) synthesized with the same conditions as the MIP but in the absence of the template molecule.…”

“…As for MIP-SPE, MIP particles for dSPE can be obtained by bulk polymerization that gives a monolith that must be crushed and possibly sieved. 53,58,64,[143][144][145][146] More homogenous MIP microspheres can also be obtained by precipitation polymerization, 63,65,[147][148][149] suspension polymerization, 45,89 and emulsion polymerization. 150 Another option is surface imprinting, which mainly consists in dispersing a core sorbent in the polymerization solution as described in detail in recent reviews.…”