Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Abstract

It has been reported in our previous work that selenized Artemisia sphaerocephala polysaccharides (SeASPs) with the Se content range of 168-1703μg/g were synthesized by using Na2SeO3/HNO3/BaCl2 system. In the present work, the solution property of SeASP was studied by using size exclusion chromatography combined with multi angle laser light scattering (SEC-MALLS). A decrease in df values indicated that SeASPs with different conformational features that were highly dependent on MW. SeASPs exhibited a more rigid conformation (df value of 1.29-1.52) in low molecular weight range (MW of 1.026-1.426×10(4)g/mol) and compact spherical conformation in high molecular weight range (MW of 2.268-4.363×10(4)g/mol). It could be due to the degradation of polysaccharide chains in HNO3, which was supported in monosaccharide composition analysis. Congo red (CR) spectrophotometric method and atomic force microscopy (AFM) results also confirmed the conformational transition and the evidence on the shape of the rigid chains. In vitro anti-tumor assays, SeASP2 displayed greater anti-proliferative effects against three tumor cell lines (hepatocellular carcinoma HepG-2 cells, lung adenocarcinom A549 cells and cervical squamous carcinoma Hela cells) in a dose-dependent manner. This suggested that selenylation could significantly enhance the anti-tumor activities of polysaccharide derivatives in vitro.

Microwave-assisted synthesis, structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide

“…The molecular height with a mean value of 2.7 nm was determined in particle analysis. AFM observation was similar to the results obtained in Section 3.4 by SEC-MALLS analysis and our previous study in selenized ASP that short chains of SeASP MW might be attributed to the lower M W caused by acid degradation [25]. Moreover, as observed in the molecular morphology of SeASP MW , it was deduced that the molecular aggregation was strong at all concentrations.…”

“…Compared to our previous report, SeASP MW with lower Se contents (111-264 μg/g) presented weaker anti-proliferative activities than selenized ASP with high Se content of 1703 μg/g [25]. Further studies on the preparation of selenized polysaccharides with different Se contents and structure features are necessary to expose the structure-bioactivity relations.…”

“…After selenylation, SeASP exhibited better scavenge hydroxyl and superoxide radical activities in vitro [24]. In anti-tumor assays, SeASP displayed greater anti-proliferative effects against HepG-2 cells, A549 cells and Hela cells in a dose-dependent manner [25].…”

Seleno-short-chain chitosan induces apoptosis in human non-small-cell lung cancer A549 cells through ROS-mediated mitochondrial pathway

“…Selenide mannan, SeGLP and Ch-SeNPs have been reported for antitumor activities in Hep G 2 cells (Decker et al 2001;Estevez et al 2014;Yang et al 1992). Accordingly, SeASP2 also displayed obvious anti-proliferation activity to three tumor cell lines including Hep G2, A549 and Hela cells (Wang et al 2016). Chitosan is a polysaccharide and is derived from partial deacetylation of chitin, which is composed of b-(1-4)-linked D-glucosamine and N-acetyl glucosamine subunits.…”

Biological Activity and Pharmacological Application of Pectic Polysaccharides: A Review

“…Artemisia sphaerocephala Krasch occurs commonly in China, and has been applied in traditional medicine to cure asthma, hepatitis, and rheumatoid arthritis [109]. A. sphaerocephala polysaccharide was isolated and purified; the carbohydrate, protein, and uronic acid contents were 90.2%, 2.4%, and 16.3%, respectively.…”