Synthesis of silver nanoparticles in context of their cytotoxicity, antibacterial activities, skin penetration and application in skincare products

Szczepańska, Elżbieta; Bielicka–Giełdoń, Aleksandra; Niska, Karolina; Strankowska, Justyna; Żebrowska, Joanna; Inkielewicz‐Stępniak, Iwona; Łubkowska, Beata; Swebocki, Tomasz; Skowron, Piotr; Grobelna, Beata

doi:10.1080/10610278.2020.1726917

Cited by 21 publications

(20 citation statements)

References 39 publications

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“…Nowadays, various metal nanoparticles such as silver, gold or titanium are widely used not only in industry, but also in nanotechnology and other various fields of research [ 7 , 8 ]. The titanium dioxide nanoparticles (TiO 2 NPs) are utilized in many fields of industry [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Efficient Method for the Concentration Determination of Fmoc Groups Incorporated in the Core-Shell Materials by Fmoc–Glycine

et al. 2020

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In this paper, we described the synthesis procedure of TiO2@SiO2 core-shell modified with 3-(aminopropyl)trimethoxysilane (APTMS). The chemical attachment of Fmoc–glycine (Fmoc–Gly–OH) at the surface of the core-shell structure was performed to determine the amount of active amino groups on the basis of the amount of Fmoc group calculation. We characterized nanostructures using various methods: transmission electron microscope (TEM), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and X-ray photoelectron spectroscopy (XPS) to confirm the modification effectiveness. The ultraviolet-visible spectroscopy (UV-vis) measurement was adopted for the quantitative determination of amino groups present on the TiO2@SiO2 core-shell surface by determination of Fmoc substitution. The nanomaterials were functionalized by Fmoc–Gly–OH and then the fluorenylmethyloxycarbonyl (Fmoc) group was cleaved using 20% (v/v) solution of piperidine in DMF. This reaction led to the formation of a dibenzofulvene–piperidine adduct enabling the estimation of free Fmoc groups by measurement the maximum absorption at 289 and 301 nm using UV-vis spectroscopy. The calculations of Fmoc loading on core-shell materials was performed using different molar absorption coefficient: 5800 and 6089 dm3 × mol−1 × cm−1 for λ = 289 nm and both 7800 and 8021 dm3 × mol−1 × cm−1 for λ = 301 nm. The obtained results indicate that amount of Fmoc groups present on TiO2@SiO2–(CH2)3–NH2 was calculated at 6 to 9 µmol/g. Furthermore, all measurements were compared with Fmoc–Gly–OH used as the model sample.

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Section: Introductionmentioning

confidence: 99%

Efficient Method for the Concentration Determination of Fmoc Groups Incorporated in the Core-Shell Materials by Fmoc–Glycine

et al. 2020

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“…Silver nanoparticles were selected to form the core due to their high enhancement efficiency of the fluorescence signal [ 31 ]. In addition, silver has an antimicrobial effect and already in small quantities exhibits a preservative effect in cosmetics, textile, and medical products [ 32 , 33 ]. Silica shell was chosen as a separator to control the distance between the DNS group used as fluorophore and the silver surface to obtain maximum fluorescence enhancement.…”

Section: Introductionmentioning

confidence: 99%

Dansyl-Labelled Ag@SiO2 Core-Shell Nanostructures—Synthesis, Characterization, and Metal-Enhanced Fluorescence

et al. 2020

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The present work describes synthesis, characterization, and use of a new dansyl-labelled Ag@SiO2 nanocomposite as an element of a new plasmonic platform to enhance the fluorescence intensity. Keeping in mind that typical surface plasmon resonance (SPR) characteristics of silver nanoparticles coincide well enough with the absorption of dansyl molecules, we used them to build the core of the nanocomposite. Moreover, we utilized 10 nm amino-functionalized silica shell as a separator between silver nanoparticles and the dansyl dye to prevent the dye-to-metal energy transfer. The dansyl group was incorporated into Ag@SiO2 core-shell nanostructures by the reaction of aminopropyltrimethoxysilane with dansyl chloride and we characterized the new dansyl-labelled Ag@SiO2 nanocomposite using transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR). Additionally, water wettability measurements (WWM) were carried out to assess the hydrophobicity and hydrophilicity of the studied surface. We found that the nanocomposite deposited on a semitransparent silver mirror strongly increased the fluorescence intensity of dansyl dye (about 87-fold) compared with the control sample on the glass, proving that the system is a perfect candidate for a sensitive plasmonic platform.

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“…In recent years, nanosilver (Ag NPs) has often been used in everyday products, including dressings [1][2][3], cleaning agents [4], cosmetics [5,6], and controlled drug delivery [7] due to its enhanced antibacterial or antifungal properties compared with macro-scale silver. Moreover, silver nanoparticles have unique properties, such as optical properties concerning the surface plasmon phenomenon [8].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, silver nanoparticles have unique properties, such as optical properties concerning the surface plasmon phenomenon [8]. Despite the many advantages of Ag NPs, there are more scientific publications indicating its toxic properties, which are influenced by many factors, including those that are most important, such as size [6], shape [9], or the type of nanoparticle synthesis [10].…”

Section: Introductionmentioning

confidence: 99%

“…One of the nanoparticles' description parameters is their potential toxicity, including the determination of an exposure route. There are several routes of human exposure to nanoparticles [28][29][30], including pulmonary [31][32][33], oral [34][35][36][37], and transdermal [6,[38][39][40]. One of the most susceptible routes of nanoparticles action is the transdermal route.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Cytotoxicity Comparison of Silver- and Silica-Based Nanostructures

et al. 2021

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Core-shell structures are the most common type of composite material nanostructures due to their multifunctional properties. Silver nanoparticles show broad antimicrobial activity, but the safety of their utilization still remains an issue to tackle. In many applications, the silver core is coated with inorganic shell to reduce the metal toxicity. This article presents the synthesis of various materials based on silver and silica nanoparticles, including SiO2@Ag, Ag@SiO2, and sandwich nanostructures—Ag@SiO2@Ag—and the morphology of these nanomaterials based on transmission electron microscopy (TEM), UV-Vis spectroscopy, and FT-IR spectroscopy. Moreover, we conducted the angle measurements due to the strong relationship between the level of surface wettability and cell adhesion efficiency. The main aim of the study was to determine the cytotoxicity of the obtained materials against two types of human skin cells—keratinocytes (HaCaT) and fibroblasts (HDF). We found that among all the obtained structures, SiO2@Ag and Ag@SiO2 showed the lowest cell toxicity and very high half-maximal inhibitory concentration. Moreover, the measurements of the contact angle showed that Ag@SiO2 nanostructures were different from other materials due to their superhydrophilic nature. The novel approach presented here shows the promise of implementing core-shell type nanomaterials in skin-applied cosmetic or medical products.

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Synthesis of silver nanoparticles in context of their cytotoxicity, antibacterial activities, skin penetration and application in skincare products

Cited by 21 publications

References 39 publications

Efficient Method for the Concentration Determination of Fmoc Groups Incorporated in the Core-Shell Materials by Fmoc–Glycine

Efficient Method for the Concentration Determination of Fmoc Groups Incorporated in the Core-Shell Materials by Fmoc–Glycine

Dansyl-Labelled Ag@SiO2 Core-Shell Nanostructures—Synthesis, Characterization, and Metal-Enhanced Fluorescence

Characterization and Cytotoxicity Comparison of Silver- and Silica-Based Nanostructures

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