Synthesis of streptavidin-conjugated magnetic nanoparticles for DNA detection

Gong, Peijun; Peng, Zheyang; Wang, Yao; Qiao, Ru; Mao, Weixing; Qian, Haisheng; Zhang, Mengya; Li, Congcong; Shi, Shenyuan

doi:10.1007/s11051-013-1558-9

Cited by 18 publications

(10 citation statements)

References 42 publications

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“…The streptavidin-coated MNPs had a characteristic plasmon resonance peak at around 536 nm, which shifted to 561 nm for the immobilized enzymes (Figure , inset). This peak indicated the formation of the biotin–streptavidin bond and the agglomeration of the streptavidin-coated MNPs . The agglomeration of the streptavidin-coated MNPs might be caused by hydrogen bonds between streptavidin molecules on different particles .…”

Section: Resultsmentioning

confidence: 97%

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Preparation of Streptavidin-Coated Magnetic Nanoparticles for Specific Immobilization of Enzymes with High Activity and Enhanced Stability

Long

Liu

et al. 2021

Ind. Eng. Chem. Res.

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In this study, streptavidin-functionalized magnetic nanoparticles were developed as a support for enzyme immobilization based on the specific recognition between biotin and streptavidin. The enzymeimmobilizing ability was evaluated using pullulanase as a model enzyme. Magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) nanoparticles were prepared and functionalized with streptavidin using cyanuric chloride. The immobilized enzymes were characterized using UV−vis spectra, Fourier-transform infrared spectroscopy, and transmission electron microscopy. The immobilized pullulanase retained high levels of activity (85.3%) and exhibited significantly improved pH and thermal stability as compared to the free enzyme. At pH 5.5, the relative activity of the immobilized enzyme (75.2%) was significantly greater than that of the free enzyme (15.8%; p < 0.01). After incubation for 360 min at 60 °C, the residual activity of the free enzyme was only 21.5%, while the immobilized enzyme retained more than 70.6% of its residual activity. Moreover, the immobilized pullulanase also exhibited excellent recyclability, retaining more than 74.2% of its initial activity after 8 consecutive reuses. These results indicated that streptavidin-coated magnetic nanoparticles have great potential for use as a support for the immobilization of the various enzymes required for continuous biotechnological applications.

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Section: Resultsmentioning

confidence: 97%

“…Oxirane groups were found by analysis of the band at 920 cm –1 due to C–O stretching. The FT-IR spectra of streptavidin showed characteristic peaks at 1639, 1549, and 1062 cm –1 (Figure a) . The peaks around 1639 and 1549 cm –1 corresponded to the stretching vibrations of the amino group and the amide I band, respectively.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Streptavidin-Coated Magnetic Nanoparticles for Specific Immobilization of Enzymes with High Activity and Enhanced Stability

Long

Liu

et al. 2021

Ind. Eng. Chem. Res.

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“…2 3 5-7 18-21 In addition, more recently the biotin STREP system was been investigated for use on other nanoparticle types, as for example for coupling of catalase on the surface of gold nanoparticles, 22 or for DNA-detection by magnetic nanoparticles. 23 Herein, a simple one-step biotin-STREP method for attachment of mAbs on LIPs was developed. Interestingly, although a very low amount of STREP was used in the proposed new technique, no vesicle aggregation occurred, as proven by the resulting vesicle size distribution.…”

Section: Papadia Et Almentioning

confidence: 99%

A Simplified Method to Attach Antibodies on Liposomes by Biotin-Streptavidin Affinity for Rapid and Economical Screening of Targeted Liposomes

Papadia¹,

Markoutsa²,

Antimisiaris³

2014

j biomed nanotechnol

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The biotin-Streptavidin (STREP) technique for attachment of monoclonal antibodies (mAbs) (or other ligand types) on liposome surface offers high attachment yield, however it is time consuming and expensive due to the number of steps used and the consumption of large quantities of STREP. Herein, a simplified, fast and economic technique, by incubating pre-mixed biotin-mAb/STREP with biotin-liposomes, at a 3:1:1 biotin-mAb/STREP/biotin-LIP ratio (mol/mol/mol) was evaluated. The physichochemical properties, final mAb attachment yield and targeting potential of liposomes decorated with an anti-transferrin receptor mAb (TfR-mAb), prepared by the simple method (SM) and the conventional method (CM), were compared. The vesicle uptake by hCMEC/D3 cells (known to overexpress TfR) were considered as a measure of liposome targeting capability. Results show that both targeted liposome types (SM and CM) have small size (mean diameters around 150 nm), low poly-dispersity (approx. 0.20) and similar mAb attachment yield (between 64-88%). However, the uptake of the SM-liposomes is slightly lower compared to CM-LIP (24-30% decrease), suggesting that the modulated conformation of mAbs on the liposome surface (triplets attached to one single STREP molecule) results in decreased targeting capability. Nevertheless, the simpler and faster one-step preparation procedure which has very high lipid recovery (> 95%) compared to the CM (50-60%) and 15-30 times lower consumption of STREP, may be a good alternative for initial screening of various mAbs as ligands for targeted liposomal or other nanotechnologies, during pre-clinical development.

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“…Then, 1 mL of glutaraldehyde (25% in H 2 O) and 0.25 mL of PBS (0.01M, pH 8.0) were added into the suspension in nitrogen atmosphere at room temperature, followed by constant stirring for 3 h. To remove excess glutaraldehyde, glutaraldehyde-modified microparticles were washed with ethanol and PBS (0.01M, pH 7.4) for three times, respectively. Then, aldehyde-modified microparticles were mixed with 0.5 mg of 1 mg mL 21 SA (in 0.01 M PBS, pH 7.4), and incubated at 4 C for 24 h. 41 Unreacted SAs were washed with PBS (0.01M, pH 7.4) and the UV spectra of the supernatant determined at 282 nm. The amount of SAs coated onto aldehydemodified microparticles could be calculated according to the method reported by literature.…”

Section: Preparation Of Sa-modified Microparticlesmentioning

confidence: 99%

Synthesis and application of streptavidin functionalized organosilica microparticles

Hou

Zhang

Wang

et al. 2014

J of Applied Polymer Sci

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This article reports on the chemical synthesis, functionalization and application of streptavidin (SA)-coated organosilica microparticles. Thiol-functionalized organosilica microparticles are synthesized via a two-step approach involving acid-catalyzed hydrolysis and condensation of 3-mercaptopropyl trimethoxysilane (MPS), followed by base-catalyzed condensation. The surfaces of these particles are modified with 3-aminopropyltriethoxysilane (APS), and characterized by FTIR, XPS, and TGA. Then, APSfunctionalized microparticles are covalently coated with SAs by using glutaraldehyde as a coupling agent, and characterized by FTIR, SEM, and AFM. Immobilization of biotinylated k DNA onto microparticles via SA-biotin immuoreaction is confirmed by confocal microscopy. The results show that these SA-functionalized organosilica microparticles are useful carriers for DNA manipulation at the single molecule level.

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Synthesis of streptavidin-conjugated magnetic nanoparticles for DNA detection

Cited by 18 publications

References 42 publications

Preparation of Streptavidin-Coated Magnetic Nanoparticles for Specific Immobilization of Enzymes with High Activity and Enhanced Stability

Preparation of Streptavidin-Coated Magnetic Nanoparticles for Specific Immobilization of Enzymes with High Activity and Enhanced Stability

A Simplified Method to Attach Antibodies on Liposomes by Biotin-Streptavidin Affinity for Rapid and Economical Screening of Targeted Liposomes

Synthesis and application of streptavidin functionalized organosilica microparticles

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