Synthesis of the 1.BETA.-hydroxylated bile acids, unusual bile acids in human biological fluids.

Capillary Gas Chromatographic Determination of C27-Bile Acids in Biological Samples and Its Application to the Urine of a Patient with Zellweger Syndrome

“…The L5-double bond may also increase about 1 MU as in the case of lb. The elution order of C27-bile acids (3,4,5) was in good agreement with that of the corresponding C24-bile acids. All MU values of the cholestanoic acids were about 2.3 MU more than those of the corresponding C24-bile acids, which indicated that the elongation (3 carbon units) of the side chain caused the MU values to increase constantly.…”

Measurement of Transport Activities of Bile Acids in Human Multidrug Resistance-Associated Protein 3 Using Liquid Chromatography-Tandem Mass Spectrometry

“…Glycine-and taurine-conjugated bile acids and bile acid 3-sulfates were all stock samples synthesized in our laboratory. [26][27][28][29] Chemical structures of 1β-and 6α-hydroxylated bile acids, unsaturated bile acids and their abbreviations are shown in Fig. 1.…”

Measurement of the Transport Activities of Bile Salt Export Pump Using LC-MS

“…Tauro-1β,3α,7α,12α-tetrahydroxy-5β-cholan-24-oic acid (T-CA-1β-ol), tauro-3α,6α,7α,12α-tetrahydroxy-5β-cholan-24-oic acid (T-CA-6α-ol), tauro-3β-hydroxy-5-cholenoic acid (T-Δ 5 -3β-ol), tauro-7α,12α-dihydroxy-3-oxo-4-cholenoic acid (T-CA-Δ 4 -3-one), tauro-1β-hydroxychenodeoxycholic acid (T-CDCA-1β-ol), tauro-2β-hydroxychenodeoxycholic acid (T-CDCA-2β-ol), ]-T-Δ 5 -3β-ol (T-Δ 5 -3β-ol-d6) were all stock samples which were synthesized in our laboratory. [17][18][19][20][21][22][23] T-CA-d6, T-CDCA-d6, T-DCA-d6, T-LCA-d6, T-Δ 5 -3β-ol-d6, T-CDCA-1β-ol and T-CDCA-2β-ol were used as internal standards (ISs) for the determination of bile acids by LC-ESI-MS. ATP and adenosine monophosphate disodium salt (AMP) were obtained from Oriental Yeast (Tokyo, Japan). Human BSEP-expressing Sf9 membrane vesicles were obtained from GenoMembrane, Inc. (Kanagawa, Japan).…”