Synthesis, surface modification and biological imaging of aggregation-induced emission (AIE) dye doped silica nanoparticles

Mao, Liucheng; Xü, Dong; Wan, Qing; Huang, Qiang; Jiang, Ruming; Shi, Yuying; Deng, Fengjie; Zhang, Xiaoyong; Wei, Yen

doi:10.1016/j.apsusc.2017.01.234

Cited by 17 publications

(7 citation statements)

References 61 publications

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“…44 AIE-active dyes have been encapsulated inside silica nanoparticles to prepare the nano-barcodes. 45,46 Very recently, a 1,8-naphthalimide derivative fluorophore, a new organic dye, was designed to prepare encoded silica nanoparticles Schemas showing the mechanisms of (a) the downconversion emission process, where a high-energy excitation photon (hn1) gets absorbed by the system in ground state 1 and excites the electron to excited state 3. The unstable electron undergoes non-radiative decay to a lower-excited state 2, followed by relaxation to the ground state accompanied by the emission of a lower-energy photon (hn2), and (b) upconversion emission processes, where excitation photon (hn1) gets absorbed by the system in ground state 1 and excites the electron to metastable excited state 2.…”

Section: Optical Encodingmentioning

confidence: 99%

“…46 Another AIEactive dye, 10-cetyl-10H-phenothiazine-3,7-(4,40-aminophenyl)acetonitrile, has also been used to prepare nano-barcodes and was demonstrated for successful uptake by HeLa cells, thereby unleashing their potential to be used for biological applications. 45 Despite the improvements, inorganic fluorescent nanoparticles such as QDs and lanthanide-doped nanocrystals have proven to be better alternatives with several advantages over organic dyes as discussed next.…”

Section: Optical Encodingmentioning

confidence: 99%

“…Recently, nano-barcodes with AIE-active dyes encapsulated inside silica nanoparticles were demonstrated for cellular uptake, displaying their promising bioimaging application. 45 In another study, AIE-active dye encoded nanobarcodes were used for imaging of mitochondria after passing the cell membranes of HeLa and KB cells, thereby further displaying their potential to image specific organelles. 46 In comparison to fluorescent barcodes, Raman barcodes have benefits of nil autofluorescence (for down-converting fluorescent particles), little spectral overlap and absence of photobleaching.…”

Section: Imagingmentioning

confidence: 99%

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Versatile design and synthesis of nano-barcodes

et al. 2017

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Encoded nano-structures/particles have been used for barcoding and are in great demand for the simultaneous analysis of multiple targets. Due to their nanoscale dimension(s), nano-barcodes have been implemented favourably for bioimaging, in addition to their security and multiplex bioassay application. In designing nano-barcodes for a specific application, encoding techniques, synthesis strategies, and decoding techniques need to be considered. The encoding techniques to generate unique multiple codes for nano-barcodes are based on certain encoding elements including optical (fluorescent and non-fluorescent), graphical, magnetic, and phase change properties of nanoparticles or their different shapes and sizes. These encoding elements can generally be embedded inside, decorated on the surface of nanostructures or self-assembled to prepare the nano-barcodes. The decoding techniques for each encoding technique are different and need to be suitable for the desired applications. This review will provide a thorough discussion on designing nano-barcodes, focusing on the encoding techniques, synthesis methods, and decoding for applications including bio-detection, imaging, and anti-counterfeiting. Additionally, associated challenges in the field and potential solutions will also be discussed. We believe that a comprehensive understanding on this topic could significantly contribute towards the advancement of nano-barcodes for a broad spectrum of applications.

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Section: Optical Encodingmentioning

confidence: 99%

Section: Optical Encodingmentioning

confidence: 99%

Section: Imagingmentioning

confidence: 99%

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Versatile design and synthesis of nano-barcodes

et al. 2017

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“…[23][24][25] AIE based dyes have also been incorporated into silica NPs for bio-imaging. [26][27][28][29] J-aggregates of a heptamethine dye have been prepared inside hollow mesoporous silica nanoparticles for NIR imaging by Sletten and coworkers. 10 The silica nanoparticles have been shown to stabilize J-aggregates and facilitate high resolution in vivo imaging in mice.…”

Section: Introductionmentioning

confidence: 99%

Ultrabright Silica-Coated Organic Nanocrystals for Two-Photon In Vivo Imaging

Perdoor

Dubois

Barbara

et al. 2020

ACS Appl. Nano Mater.

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Herein, we demonstrate the potential of ultrabright fluorescent silica-coated organic nanocrystals for two-photon in vivo imaging. These unique nanoparticles (NPs) containing a crystalline core of small push-pull dipolar dye are specifically designed to exhibit two-photon absorption for 2 fluorescence imaging. The NPs can be easily functionalized using click chemistry in pure water, with preservation of the organic core. A novel small-volume DLS technique was used to evaluate the effect of PEGylation on the colloidal stability of the NPs in complex media containing salts and proteins, mimicking the composition of blood serum. The potential of these bright red-emitting NPs for two-photon fluorescence imaging is demonstrated both in vitro and in vivo.

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“…These hybrid NPs generally gave rather weak emission because of the significant quenching caused by the aggregation of dyes in the solid state. To address this problem, luminogens with the feature of aggregation-induced emission (AIEgens), were introduced into the fabrication of fluorescent inorganic NPs via non-covalent or covalent binding, leading to enhanced fluorescence brightness and superior photostability [ 29 , 30 , 31 , 32 , 33 , 34 ]. For example, Prasad et al firstly reported AIEgens functionalized SiO 2 NPs via a physical doping method [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Facile Fabrication of Fluorescent Inorganic Nanoparticles with Diverse Shapes for Cell Imaging

Wang

Zhao

et al. 2019

Nanomaterials

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In the present work, we describe a facile and general method of fabricating fluorescent inorganic nanoparticles with diverse shapes for cell imaging application. The hematite (α-Fe2O3) nanoparticles (HNPs) with three different shapes (i.e., spindle shape, ellipsoidal shape and quasi-spherical shape) were first prepared as model systems in consideration of good biocompatibility and the controllable morphology of α-Fe2O3. Three fluorescent HNPs with different shapes were readily achieved via one-pot sol-gel reaction of AIE luminogen-functionalized siloxane (AIEgen-Si(OCH3)3) and TEOS in the presence of PVP-stabilized HNPs. Due to the fluorescence originating from the thin AIEgens-contained SiO2 shell around the HNPs, their photoluminescent intensities can be tuned by changing the concentrations of TEOS and AIEgen-Si(OCH3)3 in feed prior to the sol-gel reaction. When the as-prepared fluorescent products were dispersed in water, they gave intense green light emission upon excitation at 360 nm with relatively high fluorescence quantum yield. Further, fluorescent HNPs exhibited low cytotoxicity and excellent photostability and, thus, were used as optical probes to preliminarily explore the effect of nanoparticle shapes on their cellular uptake behaviors. This work should open a facile way to prepare various fluorescent inorganic nanoparticles with specific morphology for various biological applications.

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Synthesis, surface modification and biological imaging of aggregation-induced emission (AIE) dye doped silica nanoparticles

Abstract: Synthesis, surface modification and biological imaging of aggregation-induced emission (AIE) dye doped silica nanoparticles

Cited by 17 publications

References 61 publications

Versatile design and synthesis of nano-barcodes

Versatile design and synthesis of nano-barcodes

Ultrabright Silica-Coated Organic Nanocrystals for Two-Photon In Vivo Imaging

Facile Fabrication of Fluorescent Inorganic Nanoparticles with Diverse Shapes for Cell Imaging

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