Synthesis, X-ray absorption spectroscopy and purification of the seleno-bis (S-glutathionyl) arsinium anion from selenide, arsenite and glutathione

Gailer, Jürgen; George, Graham N.; Pickering, Ingrid J.; Buttigieg, Gavin A.; Denton, M. Bonner; Glass, Richard S.

doi:10.1016/s0022-328x(02)01192-0

Cited by 22 publications

(25 citation statements)

References 14 publications

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“…62 This compound was first discovered as seleno Bis( S -glutathionyl) arsinium ion [(GS) 2 AsSe] − . 63 It was later found that simultaneous entry of As and Se into a cell, results in the formation of (GS) 2 AsOH first, which is then attacked by HSe − , displacing its –OH group, to form [(GS) 2 AsSe] − , 64 which can later be excreted from the cell. Furthermore, Rossman and Uddin, 65 proposed that Se can guard against As induced oxidative damage through the upregulation of selenoproteins, thioredoxin reductase and glutathione peroxidase.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the cytotoxicity of Ge–Sb–Se chalcogenide glass optical fibres on 3T3 mouse fibroblasts

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Section: Discussionmentioning

confidence: 99%

Evaluating the cytotoxicity of Ge–Sb–Se chalcogenide glass optical fibres on 3T3 mouse fibroblasts

et al. 2021

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“…Analysis of the reaction mixture on a Sephadex G-10 SEC column using 0.1 M Tris-buffer (pH 8.0) as the mobile phase clearly demonstrated that (GS) 2 AsSe − had formed (this was confirmed by XAS). Thus, the investigated reaction mechanism can explain how (GS) 2 AsSe − might be formed in vivo [131]. A related trace element antagonism was discovered in 1967 when the coadministration of rats with HgCl 2 and HSeO 3 − resulted in negligible toxicity, whereas essentially all animals in the HgCl 2 group died [156].…”

Section: Interaction Of Two or Three Toxic Metals With One Endogenousmentioning

confidence: 97%

“…GSH has, in fact, been referred to as the first line of defense against the acute toxicity of Cd 2+ and Hg 2+ [128,129], and its reaction with numerous toxic metals has therefore been extensively investigated [130]. The interaction of GSH with toxic metals/metalloid compounds, however, is not only relevant with regard to their acute toxicity, but also their chronic toxicity, since the formation of complexes can result in the activation of the metal/metalloid [131]. Since Lcysteine (Cys, Figure 1(b)) is present in hepatocyte cytosol at 0.2-0.5 mM [126] and at 10 M in blood plasma [132], it represents another toxicologically relevant endogenous ligand.…”

Section: ''Bottom-up'' Lc Approaches To Probe the Interaction Of Toximentioning

confidence: 99%

Metal Species in Biology: Bottom-Up and Top-Down LC Approaches in Applied Toxicological Research

Gailer

2013

ISRN Chromatography

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Since the inception of liquid chromatography (LC) more than 100 years ago this separation technique has been developed into a powerful analytical tool that is frequently applied in life science research. To this end, unique insights into the interaction of metal species (throughout this manuscript “metal species” refers to “toxic metals, metalloid compounds, and metal-based drugs” and “toxic metals” to “toxic metals and metalloid compounds”) with endogenous ligands can be obtained by using LC approaches that involve their hyphenation with inductively coupled plasma-based element specific detectors. This review aims to provide a synopsis of the different LC approaches which may be employed to advance our understanding of these interactions either in a “bottom-up” or a “top-down” manner. In the “bottom-up” LC-configuration, endogenous ligands are introduced into a physiologically relevant mobile phase buffer, and the metal species of interest is injected. Subsequent “interrogation” of the on-column formed complex(es) by employing a suitable separation mechanism (e.g., size exclusion chromatography or reversed-phase LC) while changing the ligand concentration(s), the column temperature or the pH can provide valuable insight into the formation of complexes under near physiological conditions. This approach allows to establish the relative stability and hydrophobicity of metal-ligand complexes as well as the dynamic coordination of a metal species (injected) to two ligands (dissolved in the mobile phase). Conversely, the “top-down” analysis of a biological fluid (e.g., blood plasma) by LC (e.g., using size exclusion chromatography) can be used to determine the size distribution of endogenous metalloproteins which are collectively referred to as the “metalloproteome”. This approach can provide unique insight into the metabolism and the plasma protein binding of metal species, and can simultaneously visualize the dose-dependent perturbation of the metalloproteome by a particular metal species. The concerted application of these LC approaches is destined to provide new insight into biochemical processes which represent an important starting point to advance human health in the 21st century.

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“…17 The mechanism for formation of 7 has been studied. 18 An exciting application of the beneficial effect of selenium on arsenic toxicity is the use of dietary selenium supplements, or other forms of selenium, to counter arsenic toxicity. 19 In summary, the use of chemical synthesis, spectroscopic methods and chemical insight contributed significantly to our present understanding of the role of selenium in biology.…”

Section: Ooo-trimethylselenophosphate 2 With Trimethylsilyl Iodide mentioning

confidence: 99%

Insights into the Chemical Biology of Selenium

Glass

Berry

Block

et al. 2008

Phosphorus, Sulfur, and Silicon and the Related Elements

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Synthesis, X-ray absorption spectroscopy and purification of the seleno-bis (S-glutathionyl) arsinium anion from selenide, arsenite and glutathione

Cited by 22 publications

References 14 publications

Evaluating the cytotoxicity of Ge–Sb–Se chalcogenide glass optical fibres on 3T3 mouse fibroblasts

Evaluating the cytotoxicity of Ge–Sb–Se chalcogenide glass optical fibres on 3T3 mouse fibroblasts

Metal Species in Biology: Bottom-Up and Top-Down LC Approaches in Applied Toxicological Research

Insights into the Chemical Biology of Selenium

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