Synthetic Antimicrobial Peptide Design

Powell, William A.; Catranis, Catharine M.; Maynard, Charles

doi:10.1094/mpmi-8-0792

Cited by 83 publications

(54 citation statements)

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“…Many studies were conducted with AMPs against plant pathogens in vitro and in planta, and their advantages and disadvantages are discussed in the introduction (3,11,15,(31)(32)(33)(34)(35)(40)(41)(42). Here, we demonstrated the successful application of the ultrashort lipopeptides as fungicides for treatment of gray mold on different plant tissues.…”

Section: Discussionmentioning

confidence: 84%

“…Their membranolytic mechanism makes it difficult for the microorganisms to develop resistance (23,47,52,61). These compounds include peptides and their synthetic derivatives mimicking gene-encoded AMPs from various species as well as lipopeptides, cyclopeptides, and cyclic lipopeptides (CLPs) which are produced via nonribosomal synthesis by various microorganisms (3,(31)(32)(33)(34)(35)(40)(41)(42). In most cases, the use of synthetic peptides is limited due to high-cost production (11,19).…”

mentioning

confidence: 99%

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Inhibition of Fungal and Bacterial Plant Pathogens In Vitro and In Planta with Ultrashort Cationic Lipopeptides

Makovitzki¹,

Viterbo

Brotman

et al. 2007

Appl Environ Microbiol

102

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Section: Discussionmentioning

confidence: 84%

mentioning

confidence: 99%

Inhibition of Fungal and Bacterial Plant Pathogens In Vitro and In Planta with Ultrashort Cationic Lipopeptides

Makovitzki¹,

Viterbo

Brotman

et al. 2007

Appl Environ Microbiol

102

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“…A helical-wheel representation of this region suggested an amphipathic helix with a strongly positively charged face. While little is known concerning all the factors that contribute to the inhibitory activity of antimicrobial peptides, it is thought that amphipathic a-helical structures are important in determining the activity of some antimicrobial peptides [45,461. It is also thought that a highly positively charged and narrow polar face is needed to effect maximal antimicrobial activity [47,481.…”

Section: Discussionmentioning

confidence: 99%

Purification, Characterisation and cDNA Cloning of an Antimicrobial Peptide from Macadamia Integrifolia

Marcus

Goulter

Green

et al. 1997

European Journal of Biochemistry

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An antimicrobial peptide with no significant amino acid sequence similarity to previously described peptides has been isolated from the nut kernels of Macadamia integrifolia. The peptide, termed MiAMP1, is highly basic with an estimated pI of 10.1, a mass of 8.1 kDa and contains 76 amino acids including 6 cysteine residues. A cDNA clone containing the entire coding region corresponding to the peptide was obtained. The deduced amino acid sequence of the cDNA indicated a 26‐amino‐acid signal peptide at the N‐terminus of the preprotein. Purified MiAMP1 inhibited the growth of a variety of fungal, oomycete and gram‐positive bacterial phytopathogens in vitro. Some pathogens exhibited close to 100% inhibition in less than 1 μM peptide (5 μg/ml). Antimicrobial activity was diminished against most, but not all, microbes in the presence of calcium and potassium chloride salts (1 mM and 50 mM, respectively). MiAMPl was active against bakers yeast, was inactive against Escherichia coli and was non‐toxic to plant and mammalian cells. Analysis of genomic DNA indicated that MiAMP1 was encoded on a single copy gene containing no introns. The MiAMP1 gene may prove useful in genetic manipulations to increase disease resistance in transgenic plants.

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“…Several design strategies have been devised in order to find shorter and more stable peptides, while maintaining or increasing the activity with a low cytotoxicity. These strategies include the juxtaposition of fragments of natural antimicrobial peptides, the modification of natural peptides, and the de novo design of sequences maintaining the crucial features of native antimicrobial peptides (2,3,11,24,32,38,42). However, the process involved in the development of lead candidates is time consuming and limited by the number of individual compounds that can be synthesized.…”

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confidence: 99%

Sporicidal Activity of Synthetic Antifungal Undecapeptides and Control of Penicillium Rot of Apples

Badosa

Ferré

Francés

et al. 2009

Appl Environ Microbiol

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The antifungal activity of cecropin A(2-8)-melittin(6-9) hybrid undecapeptides, previously reported as active against plant pathogenic bacteria, was studied. A set of 15 sequences was screened in vitro against Fusarium oxysporum, Penicillium expansum, Aspergillus niger, and Rhizopus stolonifer. Most compounds were highly active against F. oxysporum (MIC < 2.5 M) but were less active against the other fungi. The best peptides were studied for their sporicidal activity and for Sytox green uptake in F. oxysporum microconidia. A significant inverse linear relationship was observed between survival and fluorescence, indicating membrane disruption. Next, we evaluated the in vitro activity against P. expansum of a 125-member peptide library with the general structure R-X 1 KLFKKILKX 10 L-NH 2 , where X 1 and X 10 corresponded to amino acids with various degrees of hydrophobicity and hydrophilicity and R included different N-terminal derivatizations. Fifteen sequences with MICs below 12.5 M were identified. The most active compounds were BP21 {Ac,F,V} and BP34 {Ac,L,V} (MIC < 6.25 M), where the braces denote R, X 1 , and X 10 positions and where Ac is an acetyl group. The peptides had sporicidal activity against P. expansum conidia. Seven of these peptides were tested in vivo by evaluating their preventative effect of inhibition of P. expansum infection in apple fruits. The peptide Ts-FK LFKKILKVL-NH 2 (BP22), where Ts is a tosyl group, was the most active with an average efficacy of 56% disease reduction, which was slightly lower than that of a commercial formulation of the fungicide imazalil.

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Synthetic Antimicrobial Peptide Design

Cited by 83 publications

References 0 publications

Inhibition of Fungal and Bacterial Plant Pathogens In Vitro and In Planta with Ultrashort Cationic Lipopeptides

Inhibition of Fungal and Bacterial Plant Pathogens In Vitro and In Planta with Ultrashort Cationic Lipopeptides

Purification, Characterisation and cDNA Cloning of an Antimicrobial Peptide from Macadamia Integrifolia

Sporicidal Activity of Synthetic Antifungal Undecapeptides and Control of Penicillium Rot of Apples

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