Synthetic biology-powered microbial co-culture strategy and application of bacterial cellulose-based composite materials

Jin, Kai; Jin, Chenyang; Wu, Yihan

doi:10.1016/j.carbpol.2022.119171

Cited by 31 publications

(26 citation statements)

References 157 publications

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“…As a rule, one of the polymers is used as a matrix or carrier, in which another polymer is included [ 2 ]. There are several main strategies and methods for developing new materials with a combination of the properties of individual components, which makes it possible to create composites with targeted activity [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…The second group—chemical modifications (molecular modification, polymer grafting, modification of pore structure). Using these two techniques, it is possible to change the hydrophobicity of the matrix, which is crucial for enhancing cell adhesion during the creation of composites [ 3 ]. The third group comprises biological methods, which are often referred to as synthetic approaches to BC composites since, in these methods, the functionalization of BC occurs either during or after its biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

“…The third group comprises biological methods, which are often referred to as synthetic approaches to BC composites since, in these methods, the functionalization of BC occurs either during or after its biosynthesis. These methods, in turn, can also be divided into three main types: in situ synthesis, ex situ synthesis, and BC dissolution [ 3 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, the BC dissolution method reconstructs its three-dimensional structure. BC is first dissolved in an organic solvent, the reinforcing materials are dispersed in it, and then the BC composite film is regenerated from the solution [ 3 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…If a biopolymer synthesized by another microorganism is included in the BC matrix, this product of microbial biosynthesis can be integrated with BC. In that way, the composite can be obtained by cocultivation of producers, creating an artificial symbiotic system [ 3 ]. This approach, in our opinion, represents an attractive, cost-effective scheme with fewer pretreatment and purification steps, since the additives are biosynthesized by microorganisms and directly incorporated into the growing BC matrix.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Advanced “Green” Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy

et al. 2022

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Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used. The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms. The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity. In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan. BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium. The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy. Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties. The developed approach for “grafting” of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Advanced “Green” Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy

et al. 2022

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Efficient Production of Bacterial Cellulose Using Komagataeibacter sucrofermentans on Sustainable Feedstocks

Liu,

Siddique,

Wei

et al. 2024

ChemSusChem

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The production of bacterial cellulose (BC) has indeed garnered global attention due to its versatile properties and applications. Despite potential benefits, the challenges like low productivity, high fermentation costs, and expensive culture media hinder its industrialization. Utilizing low‐cost substrates, especially waste streams, can help address the challenges. In this study, waste feedstocks such as restaurant leftovers, oranges, and grapefruit from canteens and supermarkets were valorized for BC production by Komagataeibacter sucrofermentans. Orange juice is a fascinating substrate with a highest concentration of 20.6 g/L and productivity of 2.05 g/L/d. Using HS medium with supplementary ions, organic acids, ethanol, and various carbon sources is a strategic approach for enhancing BC production. The study reveals that the addition of organic acids or ethanol moderately increased BC production, while ions inhibit BC synthesis, highlighting the complex interplay between various cultivation medium components. Additionally, fermentation with K. sucrofermentans using single and mixed carbon sources was conducted to elucidate the potential metabolic mechanism of BC production. Through alkaline treatment and drying in a 30 °C incubator, we produced the highest quality BC with 92.09% crystallinity. Overall, the study enhances BC production knowledge and provides green and sustainable strategies for fermentative BC production.

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Graphene‐Based Engineered Living Materials

Allahbakhsh,

Gadegaard,

Ruiz

et al. 2023

Small Methods

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With the rise of engineered living materials (ELMs) as innovative, sustainable and smart systems for diverse engineering and biological applications, global interest in advancing ELMs is on the rise. Graphene‐based nanostructures can serve as effective tools to fabricate ELMs. By using graphene‐based materials as building units and microorganisms as the designers of the end materials, next‐generation ELMs can be engineered with the structural properties of graphene‐based materials and the inherent properties of the microorganisms. However, some challenges need to be addressed to fully take advantage of graphene‐based nanostructures for the design of next‐generation ELMs. This work covers the latest advances in the fabrication and application of graphene‐based ELMs. Fabrication strategies of graphene‐based ELMs are first categorized, followed by a systematic investigation of the advantages and disadvantages within each category. Next, the potential applications of graphene‐based ELMs are covered. Moreover, the challenges associated with fabrication of next‐generation graphene‐based ELMs are identified and discussed. Based on a comprehensive overview of the literature, the primary challenge limiting the integration of graphene‐based nanostructures in ELMs is nanotoxicity arising from synthetic and structural parameters. Finally, we present possible design principles to potentially address these challenges.

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Synthetic biology-powered microbial co-culture strategy and application of bacterial cellulose-based composite materials

Cited by 31 publications

References 157 publications

Advanced “Green” Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy

Advanced “Green” Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy

Efficient Production of Bacterial Cellulose Using Komagataeibacter sucrofermentans on Sustainable Feedstocks

Graphene‐Based Engineered Living Materials

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