Synthetic boundary crystallization ultracentrifugation: a new method for the observation of nucleation and growth of inorganic colloids and the determination of stabilizer efficiencies

Börger, Lars; Cölfen, Helmut; Antonietti, Markus

doi:10.1016/s0927-7757(99)00427-6

Cited by 22 publications

(26 citation statements)

References 21 publications

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“…Examples for this are the swelling pressure equilibrium experiments 78,79 -a special case of sedimentation-diffusion equilibrium experiments for gels-or synthetic boundary crystallization experiments, which are a special case of band centrifugation experiments for the in situ formation of crystals. 80,81 Sedimentation velocity experiment A sedimentation velocity run, generally carried out at high centrifugal fields, is the most important AUC experiment (Fig. 4, graph A) for nanoparticle characterization, since particle size distributions can be obtained from sedimentation coefficient distributions.…”

Section: Basic Auc Experimentsmentioning

confidence: 99%

Analytical ultracentrifugation of colloids

2010

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In this contribution the use of Analytical Ultracentrifugation (AUC) for the modern analysis of colloids is reviewed. Since AUC is a fractionation technique, distributions of the sedimentation coefficient, particle size and shape, molar mass and density can be obtained for particle sizes spanning the entire colloidal range. The Ångström resolution and the reliable statistics with which particle size distributions can be obtained from analytical ultracentrifugation makes this a high resolution analysis technique for the characterization of nanoparticles in solution or suspension. Several examples showing successful applications of AUC to complex problems in colloid science are given to illustrate the broad range and versatility of questions that can be answered by AUC experiments.

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Section: Basic Auc Experimentsmentioning

confidence: 99%

Analytical ultracentrifugation of colloids

2010

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“…Overlaying the two reactants of a crystallizing species in an AUC cell by the technique of synthetic boundary crystallization ultracentrifugation (Borger et al 2000) allows to initiate the reaction in a sharp boundary formed upon speeding up the ultracentrifuge. Since one reactant is consumed within seconds, further nucleation and growth are quenched and the formed early nucleation and growth species get then fractionated by the ultracentrifugal field and can then be detected.…”

Section: Mwl-auc In the Scientific Literaturementioning

confidence: 99%

The Multiwavelength UV/Vis Detector: New Possibilities with an Added Spectral Dimension

Karabudak

Cölfen

2016

Analytical Ultracentrifugation

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The multiwavelength (MWL) detector is a new type of absorption detector for AUC. The commercial absorption detector of the Beckman Coulter XL-A AUC can only handle a single wavelength per scan with the possibility to scan at maximum 3 wavelengths, whereas MWL-AUC can handle all the wavelengths in the UV/Vis region at one time. The result is impressive since now a full spectral dimension is added to each single scan. In this chapter, we are explaining development history, instrumentation, and future perspective of MWL-AUC.

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“…Another example for application of ABC to perform a chemical reaction directly in the AUC cell is the so-called "Synthetic boundary crystallization" (Börger and Cölfen 1999;Börger et al 2000). Here, a sodium sulfide solution is layered on top of a cadmium chloride solution containing thioglycerol as stabilizer.…”

Section: Introductionmentioning

confidence: 99%

Analytical band centrifugation revisited

Schneider

Cölfen

2018

Eur Biophys J

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Analytical band centrifugation (ABC) is a powerful tool for the analysis of macromolecules and nanoparticles. Although it offers several advantages over the sedimentation velocity (SV) experiment like a physical separation of the individual components and the possibility to perform chemical reactions, its analysis is still very restricted. Therefore, we investigated the integration of ABC data as an alternative approach, as this results in data similar to SV, which can then be evaluated by many established evaluation programs. We investigated this method using two different test systems, myoglobin as a biopolymer with significant diffusion and 100 nm polystyrene latex as a large particle with negligible diffusion, and found some limiting issues. These are namely, broadening of the initial boundary by diffusion of the sample, which can be taken into account and the dynamic density gradient between the solvent in the sector and the overlaid solution, which deforms the initial band upon movement through the gradient and is currently not taken into account. We show the influence these two factors have on the evaluation and show that it is possible to calculate the time-dependent change in solvent density and viscosity in the AUC cell using the integrated form of Fick's second law. We conclude that taking the dynamic density gradient into account will open ABC for the sophisticated methods based on the analysis of the whole sedimentation boundary and not just the determination of an average sedimentation coefficient.

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Synthetic boundary crystallization ultracentrifugation: a new method for the observation of nucleation and growth of inorganic colloids and the determination of stabilizer efficiencies

Cited by 22 publications

References 21 publications

Analytical ultracentrifugation of colloids

Analytical ultracentrifugation of colloids

The Multiwavelength UV/Vis Detector: New Possibilities with an Added Spectral Dimension

Analytical band centrifugation revisited

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