Synthetic estrogen bioaccumulates and changes the behavior and biochemical biomarkers in adult zebrafish

Tamagno, Wagner Antonio; Sofiatti, Jéssica Reis de Oliveira; Alves, Carla; Sutorillo, Nathália Tafarel; Vanin, Ana Paula; Bilibio, Denise; Pompermaier, Aline; Barcellos, Leonardo José Gil

doi:10.1016/j.etap.2022.103857

Cited by 15 publications

(6 citation statements)

References 55 publications

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“…Cholinergic signaling is responsible for learning, and locomotion, and is important in several processes, such as memory, neuronal development, and participation in the reward system 46 . Even more alterations in central systems are observed to understand and explain different disorders 47 . Al is considered a neurotoxic compound once is related to cholinergic damage in animals 15 .…”

Section: Discussionmentioning

confidence: 99%

“…THC and CBD analyte quantification were determined in the water as described by Tamagno et al, (2022) 47 with some adaptations when the fish remained for the duration of exposure. The extraction was performed using QuEChERS Extraction (Agilent Technologies, catalog number 5982-0755) and QuEChERS Dispersive kits (Agilent Technologies, catalog number 5982-4950).…”

Section: Methodsmentioning

confidence: 99%

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Cannabis sativa-based oils against aluminum-induced neurotoxicity

Alves

Tamagno

Vanin

et al. 2023

Sci Rep

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The use of terpenoid compounds in different neural-related conditions is becoming useful for several illnesses. Another possible activity of these compounds is the reduction of nervous impairment. Cannabis sativa plants are known for their concentration of two important terpenoids, the delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). CBD and THC have central peripheral activities already described and their usage in different brain diseases, such as Alzheimer's and multiple sclerosis. Aluminum (Al) is known as an important neurotoxic compound, the physiological action of Al is not known already, and in high concentrations can lead to intoxication and cause neurotoxicity. Here we evaluated the potential effect of two different doses of CBD- and THC-rich based oils against Al-induced toxicity, in the zebrafish model. We evaluated behavioral biomarkers of the novel tank test (NTT) and social preference test (SPT), and biochemical markers: the activity of the enzyme acetylcholinesterase (AChE) and the antioxidant enzymes—catalase, superoxide dismutase, and glutathione-S-transferase. CBD- and THC-based oils were able to increase the AChE activity helping the cholinergic nervous system actuate against Al toxicity which was reflected by the behavioral biomarkers changes. We concluded that the oils have a protective effect and might be used with proposals for neurological and antioxidant impairment avoidance caused by Al intoxications.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Cannabis sativa-based oils against aluminum-induced neurotoxicity

Alves

Tamagno

Vanin

et al. 2023

Sci Rep

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“…There is broad literature revising how reproductive and social behavior are impaired by 17α-ethinylestradiol (EE2), an oral contraceptive that is found in wastewater and effluents ( 150 ). Exposure to EE2 has an anxiolytic effect in adult zebrafish ( 151 ) and reduces dominant male aggression, impairing dominance and their reproductive success in half of exposed fish ( 70 ). Moreover, EE2 reduces females’ court behavior toward males and may have severe implications on the reproductive success of exposed zebrafish populations ( 152 ).…”

Section: Sex Steroids and Agonistic Behaviormentioning

confidence: 99%

Social behaviors as welfare indicators in teleost fish

2023

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Animal welfare is a key issue not only for aquaculture industry and food production, but also for daily husbandry practices in research topics related to physiology in wild and farmed animals. In this context, teleost fish constitute interesting models to assess alternative welfare indicators because of their wide diversity in reproductive and social structures. Any framework for assessing teleost fish welfare needs to account for the physiological mechanisms involved in each species as a first step. A comprehensive approach should also take into account how these physiological and behavioral parameters can be altered by environmental enrichment considering the specific requirements in each case and identifying intrinsic biological characteristics of individual species. This review will show how cortisol and sex steroids regulate social behavior in teleost fish, and how different aspects of social behavior can be employed as welfare indicators according to specific characteristics in each case. This article will consider evidence in teleost fish, including cichlids, characids and cyprinids with different reproductive strategies and social structures (e.g., territorial social hierarchies or shoaling behavior). Neotropical species will be particularly emphasized. The main laboratory-based animal welfare indicators are cortisol, a classical stress hormone, together with sex steroids. Considering that the endocrine landscape is intrinsically related to social behavior, reproductive and agonistic behavioral traits such as aggression, anxiety and courtship are key elements to assess welfare under housing and culture conditions. This review highlights the importance of assessing physiological mechanisms and identifying behavioral characteristics in teleost fish, especially in Neotropical species, as a baseline to understand which environmental enrichment can improve animal welfare in each individual species.

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“…Although filtration systems are an option for treating collected water, these systems do not remove many contaminants. In addition, there are many reports of impacts on zebrafish behavior, physiology, reproduction, and development for drug residues (48,49) and agrochemicals (50) .…”

Section: Source Of Watermentioning

confidence: 99%

Good practices in the rearing and maintenance of zebrafish (Danio rerio) in Brazilian laboratories

et al. 2023

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Good Laboratory Practice (GLP) is a management quality control system that encompasses the organizational process and conditions under which non-clinical health and environmental studies are carried out. According to the World Health Organization, GLP must contain five topics: resources, characterization, rules, results, and quality control. This work aims to address a review according to WHO standards of implementing Good Laboratory Practices in zebrafish (Danio rerio) vivariums. Considering that the promotion of one health (animal, human, and environmental) associated with an education plan, protocols, and records are fundamental to guarantee the safety and integrity of employees, animals, and the environment as well as reliability in the results generated. In a way, Brazil still needs improvements related to the well-being of aquatic organisms (national laws, international agreements, corporate programs, and others), especially concerning its use in research and technological development. In this way, the implementation of GLPs provides valuable guidance for improving animal welfare and worker safety, facilitating the standardization of research.

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Synthetic estrogen bioaccumulates and changes the behavior and biochemical biomarkers in adult zebrafish

Cited by 15 publications

References 55 publications

Cannabis sativa-based oils against aluminum-induced neurotoxicity

Cannabis sativa-based oils against aluminum-induced neurotoxicity

Social behaviors as welfare indicators in teleost fish

Good practices in the rearing and maintenance of zebrafish (Danio rerio) in Brazilian laboratories

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