Synthetic β-Barrel by Metal-Induced Folding and Assembly

Abstract

The de novo construction of repeat proteins has received much attention from biologists and chemists, yet that of a β-barrel structure, one of the most well-known classes, has not been accomplished to date. Here, we report the first chemical construction of a β-barrel tertiary structure with a pore through a combination of peptide folding and metal-directed self-assembly. Coordination of zinc salts to an eight-residue peptide fragment bearing β-strand- and loop-forming sequences resulted in a β-barrel in which six-stranded cylindrical antiparallel β-sheets formed a hydrophobic pore with a specific shape.

Metal‐Helix Frameworks from Short Hybrid Peptide Foldamers

“…[1,2] Inspired by the role of metals in the structure and functions of various types of proteins and peptides,extensive efforts have been made in the literature to design metal coordinated assemblies of proteins [3] and synthetic peptides. Nevertheless,F ujita and co-workers have demonstrated the metal driven stunning protein like channels, [6] bbarrels [7] as well as peptide catenanes [8] from short a-peptides. Nevertheless,F ujita and co-workers have demonstrated the metal driven stunning protein like channels, [6] bbarrels [7] as well as peptide catenanes [8] from short a-peptides.…”

“…[4,5] Them etal triggered assembly of peptides [5d,e] and proteins [3e] offer ac ategorical approach to construct well defined nanostructures.H owever,u tilization of peptides to construct ordered metal-peptide-frameworks are scarcely examined. Nevertheless,F ujita and co-workers have demonstrated the metal driven stunning protein like channels, [6] bbarrels [7] as well as peptide catenanes [8] from short a-peptides. Thep rotein like quaternary structures have also been achieved from the metal coordinated assembly of synthetic coiled-coils [9] and collagen peptides.…”

Intra- and intermolecular self-assembly of a 20-nm-wide supramolecular hexagonal grid

“…1). The initial intramolecular self-assembly process, which is reminiscent of the folding process of peptides or proteins 25,26 , occurs on complexation with Fe 2+ metal ions. Subsequent intermolecular self-assembly (also governed by complexation with Fe 2+ ions) leads to the formation of discrete 2D grids.…”

Metal–peptide rings form highly entangled topologically inequivalent frameworks with the same ring- and crossing-numbers

“…There are great advantages to the rapidly developing DNA nanotechnology method 22,23 , which can be used to manipulate a variety of advanced entangled nanostructures, but this method also has disadvantages, including its poor efficiency in three-dimensional shape construction, structural modification, and large-scale synthesis. However, these problems can be addressed by our folding-and-assembly method 16,20,24–26 if the structural prediction is accompanied hereafter. Considering the abundance of highly entangled nanostructures in biological systems and their contribution to the formation and stabilisation of giant protein assemblies 27–30 , molecular entanglements can be considered a new nanoscale bonding pattern representing the principles of nature.…”