System peaks in capillary zone electrophoresis of anions with negative voltage polarity and counter‐electroosmotic flow

Sursyakova, Viktoria V.; Kalyakin, Sergey N.; Burmakina, Galina V.; Rubaylo, Anatoly I.

doi:10.1002/elps.201000313

Cited by 15 publications

(15 citation statements)

References 57 publications

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“…For the negative voltage polarity, the theoretical migration time of the system peak t spi was calculated as follows :

t_{sp i} = t_{normali} \cdot \frac{l}{l_{eff}} + t_{EOF}

where t i is the migration time of the ion producing the system peak, t EOF is the migration time of the EOF marker or time corresponding to sp0. sp0 is the system peak forming at the capillary outlet in the start after the voltage application which is transferred to the detector by EOF .…”

Section: Methodsmentioning

confidence: 99%

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New peak broadening parameter for the characterization of separation capability in capillary electrophoresis

Sursyakova

Rubaylo

2015

J of Separation Science

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The influence of separation conditions on peak broadening is usually estimated by the number of theoretical plates. Using the data available in literature and experimental data, it is shown that in pressure-assisted capillary electrophoresis the plate number is not directly related to the separation capability of conditions used. The experiments at different electrolyte flow velocities demonstrate that a higher plate number (the best separation efficiency) can be obtained with a lower peak resolution. Since ions are separated by electrophoresis due to the difference in electrophoretic mobilities, the peak width in terms of electrophoretic mobility is suggested as a new peak broadening parameter describing the separation capability of the conditions used. The parameter can be calculated using the tailing factor and the temporal peak width at 5% of the peak height. A simple equation for the resolution calculation is derived using the parameter. The advantage of the peak width in terms of mobility over other parameters is shown. The new parameter is recommended to be used not only in pressure-assisted capillary electrophoresis but also in general capillary electrophoresis when in a number of runs the virtual separative migration distance and separation capability of the conditions used change widely.

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“…For the negative voltage polarity, the theoretical migration time of the system peak t spi was calculated as follows :

t_{sp i} = t_{normali} \cdot \frac{l}{l_{eff}} + t_{EOF}

Section: Methodsmentioning

confidence: 99%

“…For the negative voltage polarity, the electrophoretic mobility of the i th anion μ ep,i was calculated as follows :

\begin{matrix} true \\ right μ_{ep, i} & = & left μ_{tot, normali} + μ_{EOF + normalP} = μ_{tot, normali} + μ_{EOF} - υ_{P} \cdot \frac{l}{U} = \frac{l}{U} \\ left (\frac{l_{eff}}{t_{i}} + \frac{l - l_{eff}}{t_{EOF}}) \end{matrix}

where μ EOF is the electroosmotic mobility, υ Р is the hydrodynamic flow velocity.…”

Section: Methodsmentioning

confidence: 99%

New peak broadening parameter for the characterization of separation capability in capillary electrophoresis

Sursyakova

Rubaylo

2015

J of Separation Science

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“…For the minimization of the formation of system peaks [15,16], it would be most convenient to use background electrolyte based only on the ligand. The possibility of using EDTA as the background electro lyte and ligand was studied.…”

Section: Resultsmentioning

confidence: 99%

Determination of iron and copper ions in cognacs by capillary electrophoresis

et al. 2015

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A method is developed for the determination of iron(III) and copper(II) in cognacs by capillary electrophoresis (CE) with diode array detection with limits of detection 0.06 and 0.6 mg/L, respectively. For eign substances present in cognac do not interfere with the determination. The accuracy of the results of anal ysis was confirmed by the added-found method. The method was applied to the analysis of seven cognac samples. The data obtained by CE are in a good agreement with the results of inductively coupled plasma mass spectrometry.

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“…The detection wavelengths were 254 and 191 nm. The hydrodynamic pressure of 50 mbar was used to inject samples for 2 s and to reduce migration time during the separation [41][42][43][44].…”

Section: Instrumentationmentioning

confidence: 99%

Composition and stability constants of copper(II) complexes with succinic acid determined by capillary electrophoresis

Sursyakova

Burmakina

Rubaylo

2016

Journal of Coordination Chemistry

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Fax: 73912494108 2 Composition and stability constants of copper(II) complexes with succinic acid ions determined by capillary electrophoresisThe advantage of usage of capillary electrophoresis technique was demonstrated for studying a complicated system owing to the dependence of direction and velocity of the electrophoretic movement on the charge of complex species. The stability constants of copper(II) complexes with ions of succinic acid were determined by capillary electrophoresis, including the 1:2 metal to ligand complexes which are rarely mentioned and taken into account in studies during recent years. The measurements were carried out at 25 ºC and ionic strength of 0.1, obtained by mixing the solutions of succinic acid and lithium hydroxide up to рН 4.2 -6.2. It was shown that while pH was more than 4.5 the zone of copper(II) complexes with succinate ions moves as an anion. It is impossible to treat this fact using only the complexes with a metal-ligand ratio of 1:1 (CuL 0 , CuHL + ).The following values of stability constants were obtained: log β(CuL) = 2.89 ± 0.02, log β(CuHL + ) = 5.4 ± 0.5, log β(CuL 2 2-) = 3.88 ± 0.05, log β(CuHL 2 -) = 7.2 ± 0.3.

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System peaks in capillary zone electrophoresis of anions with negative voltage polarity and counter‐electroosmotic flow

Cited by 15 publications

References 57 publications

New peak broadening parameter for the characterization of separation capability in capillary electrophoresis

New peak broadening parameter for the characterization of separation capability in capillary electrophoresis

Determination of iron and copper ions in cognacs by capillary electrophoresis

Composition and stability constants of copper(II) complexes with succinic acid determined by capillary electrophoresis

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