Systematic analysis reveals O-methyltransferase gene family members involved in flavonoid biosynthesis in grape

Lu, Suwen; Zhuge, Yaxian; Hao, Tianyi; Liu, Zhongjie; Zhang, Mengwei; Fang, Jinggui

doi:10.1016/j.plaphy.2022.01.007

Cited by 19 publications

(11 citation statements)

References 45 publications

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“…ShCOMT is responsible for regulating the conversion of caffeic acid to ferulic acid, while ShCCoMT is responsible for converting caffeoyl-CoA to feruloyl-CoA 49,50 . These two OMT enzyme play an important regulatory role in the synthesis of podophyllotoxin precursor compound pinobanksin, and have been isolated in multiple plants 51,52 . However, the regulatory role of ShOMT3 and ShOMT1 in the podophyllotoxin biosynthetic process has only been discovered in recent years, and related studies have not yet been conducted indepth.…”

Section: Discussionmentioning

confidence: 99%

Cloning and bioinformatics analysis of key gene ShOMT3 of podophyllotoxin biosynthesis pathway in Sinopodophyllum hexandrum

Gao,

Zhao,

et al. 2024

Preprint

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Sinopodophyllum hexandrum (S. hexandrum) is an endangered traditional Chinese medicine as abundant podophyllotoxin with powerful anticancer activity. In this study, the rootstalks of S. hexandrum from different geographical locations in China [S1 (Gansu) and S2 (Shaanxi)] were used as research materials to clone the key gene pluviatolide O-methyltransferase 3 (ShOMT3) in the podophyllotoxin biosynthetic pathway. Subsequently, bioinformatics analysis of the ShOMT3 gene and its encoded protein was subjected to bioinformatics analysis using various analysis software including ProtParam, Tmhmm Server 2.0, SubLoc, Signal-P 5.0, and Swiss-model. The results of the analysis revealed that the CDS region of the ShOMT3 gene is 1119 bp long, encoding 372 amino acids. The theoretical molecular weight of the ShOMT3 protein is 41.32784 kD, and the theoretical isoelectric point (pI) is 5.27. The instability coefficient of the protein is 46.05, the aliphatic index is 93.58, and the grand average of hydropathicity (GRAVY) is 0.037, indicating that it is an unstable hydrophobic protein. The protein does not contain transmembrane domains or signal peptides, indicating that it is a non-secreted protein. Secondary structure prediction results suggests that the protein consists of alpha helices, random coils, extended strands, and beta-turns. Tertiary structure prediction results suggests that the protein functions as a monomer. In the phylogenetic tree, the ShOMT3 protein has the highest homology with Podophyllum peltatum (P. peltatum). The successful cloning and bioinformatics analysis of the ShOMT3 gene provide theoretical basis and excellent genetic resources for the molecular regulatory mechanism analysis of the podophyllotoxin biosynthetic pathway and molecular breeding in S. hexandrum.

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Section: Discussionmentioning

confidence: 99%

Cloning and bioinformatics analysis of key gene ShOMT3 of podophyllotoxin biosynthesis pathway in Sinopodophyllum hexandrum

Gao,

Zhao,

et al. 2024

Preprint

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“…COMT from soybean, tomato, apple, and grape were separated into clades D and E with distant related indicating the evolutionary constancy of COMTs between species and their functional differentiation. Differential expression patterns of COMT in different tissues of soybean and grape also suggested their functional diversity in response to abiotic stress 98,99 . Similarly, COMT in apple to a common ancestor has undergone duplication, generating two clusters with different functions 100 .…”

Section: Melatonin Biosynthesismentioning

confidence: 99%

“…Differential expression patterns of COMT in different tissues of soybean and grape also suggested their functional diversity in response to abiotic stress. 98,99 Similarly, COMT in apple to a common ancestor has undergone duplication, generating two clusters with different functions. 100 In addition, tandem or segmental duplication of COMT in Arabidopsis implied their conservative functions in coping with abiotic stress.…”

Section: Abiotic Stressmentioning

confidence: 99%

Genetic and evolutionary dissection of melatonin response signaling facilitates the regulation of plant growth and stress responses

Gao

Chen

et al. 2023

Journal of Pineal Research

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The expansion of gene families during evolution could generate functional diversity among their members to regulate plant growth and development. Melatonin, a phylogenetically ancient molecule, is vital for many aspects of a plant's life. Understanding the functional diversity of the molecular players involved in melatonin biosynthesis, signaling, and metabolism will facilitate the regulation of plant phenotypes. However, the molecular mechanism of melatonin response signaling elements in regulating this network still has many challenges. Here, we provide an in‐depth analysis of the functional diversity and evolution of molecular components in melatonin signaling pathway. Genetic analysis of multiple mutants in plant species will shed light on the role of gene families in melatonin regulatory pathways. Phylogenetic analysis of these genes was performed, which will facilitate the identification of melatonin‐related genes for future study. Based on the abovementioned signal networks, the mechanism of these genes was summarized to provide reference for studying the regulatory mechanism of melatonin in plant phenotypes. We hope that this work will facilitate melatonin research in higher plants and finely tuned spatio‐temporal regulation of melatonin signaling.

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“…Under certain conditions, these hydroxyl groups are further methylated by O-methyltransferase (OMT) to produce other anthocyanosides such as peonidin 3-Oglucoside, petunidin 3-O-glucoside, and malvidin 3-O-glucoside (Fig. 1) [23].…”

Section: Introductionmentioning

confidence: 99%

De novo biosynthesis of anthocyanins in Saccharomyces cerevisiae using metabolic pathway synthases from blueberry

Mei,

Hua,

Liu

et al. 2024

Preprint

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Background Anthocyanins are water-soluble flavonoids in plants, which give plants bright colors and are widely used as food coloring agents, nutrients, and cosmetic additives. There are several limitations for traditional techniques of collecting anthocyanins from plant tissues, including species, origin, season, and technology. The benefits of using engineering microbial production of natural products include ease of use, controllability, and high efficiency. Results In this study, ten genes of anthocyanins synthetic pathway were successfully cloned from anthocyanin-rich plant materials blueberry fruit and purple round eggplant rind. The Yeast Fab Assembly technology was utilized to construct the transcriptional units of these genes under different promoters. The transcriptional units of PAL and C4H, 4CL and CHSwere fused and inserted into Chr. XVI and IV of yeast strain JDY52 respectively using homologous recombination to gain Strain A. The fragments containing the transcriptional units of CHI and F3H, F3'H and DFRwere inserted into Chr. III and XVI to gain Strain B1. Strain B2 has the transcriptional units of ANS and 3GT in Chr. IV. Several anthocyanins, including cyanidin, peonidin, pelargonidin, petunidin, and malvidin, were detected by LC-MS/MS following the predicted outcomes of the de novo biosynthesis of anthocyanins in S. cerevisiae using a multi-strain co-culture technique. ConclusionWe propose a novel concept for advancing the heterologous De novo anthocyanin synthesis pathway, as well as fundamental information and a theoretical framework for the ensuing optimization of the microbial synthesis of anthocyanins.

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Systematic analysis reveals O-methyltransferase gene family members involved in flavonoid biosynthesis in grape

Cited by 19 publications

References 45 publications

Cloning and bioinformatics analysis of key gene ShOMT3 of podophyllotoxin biosynthesis pathway in Sinopodophyllum hexandrum

Cloning and bioinformatics analysis of key gene ShOMT3 of podophyllotoxin biosynthesis pathway in Sinopodophyllum hexandrum

Genetic and evolutionary dissection of melatonin response signaling facilitates the regulation of plant growth and stress responses

De novo biosynthesis of anthocyanins in Saccharomyces cerevisiae using metabolic pathway synthases from blueberry

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