Systematic assessment of the stability of benzaldehyde lyase in aqueous–organic biphasic systems and its stabilization by modification with methoxy-poly(ethylene) glycol

Wittenboer, Anne van den; Niemeijer, Bernd; Karmee, Sanjib Kumar; Ansorge‐Schumacher, Marion B.

doi:10.1016/j.molcatb.2010.08.007

Cited by 22 publications

(13 citation statements)

References 42 publications

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“…As illustrated in Figure 2 c, native BAL dissolved in a liquid two-phase system retained a rather low activity under these conditions (0.93 μ mol h − 1 ), which is in accordance with the reported sensitivity of this enzyme against organic solvent molecules dissolved in the aqueous phase, the interfaces between aqueous and organic phases, and even its own substrate molecules. [ 30 ] In contrast, in the Pickering emulsion, the apparent activity of BAL increased to 7.73 μ mol h − 1 , which is more than eight times higher than that of native BAL. This suggests that the drastic increase of the interfacial area by Pickering emulsifi cation largely offsets the negative impact of the reaction system on the enzyme, at least within the investigated time range.…”

Section: Methodsmentioning

confidence: 90%

Nanoparticle Cages for Enzyme Catalysis in Organic Media

et al. 2011

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Encapsulation of enzymes in Pickering emulsions results in a large interfacial area of the enzyme-containing aqueous phase for biocatalysis in organic media. This immobilization technique minimizes enzyme inactivation through stabilizing immiscible liquids by particles, facilitates separation processes, and significantly increases catalytic performance of both stable and vulnerable enzymes. Thus, a broad technical applicability can be envisioned.

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Section: Methodsmentioning

confidence: 90%

Nanoparticle Cages for Enzyme Catalysis in Organic Media

et al. 2011

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“…Organic–aqueous biphasic catalysis is an important class of environment friendly processes because it provides a way of heterogenizing the catalysts and substrates/products into two separate and immiscible phases for ready separability . Despite many advantages, the reaction rate of such systems is limited by the small organic/aqueous interfacial area …”

Section: Methodsmentioning

confidence: 99%

Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions

et al. 2018

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Despite the rapid development of Pickering interfacial catalysis (PIC) at liquid-liquid interfaces with chemocatalysts, the use of unstable biocatalysts at emulsion interfaces remains a technical challenge. Herein, we present a Pickering interfacial biocatalysis (PIB) platform based on robust and recyclable enzyme-polymer conjugates that act as both catalytic sites and stabilizers at the interface of Pickering emulsions. The conjugates were prepared by growing poly(N-isopropylacrylamide) on a fragile enzyme, benzaldehyde lyase, under physiological conditions. The mild in situ conjugation process preserved the enzyme structure, and the conjugates were used to emulsify a water-organic two-phase system into a stable Pickering emulsion, leading to a significantly larger interfacial area and a 270-fold improvement in catalytic performance as compared to the unemulsified two-phase system. The PIB system could be reused multiple times. Conjugates of other enzymes were also fabricated and applied for cascade reactions.

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“…2 The use of these media makes possible many chemical reactions that are not feasible in aqueous solution, diminish undesirable side reactions, reduce hydrolytic reactions in favor of synthesis, and facilitate product recovery 3. Three major principles are frequently applied in biocatalysis: monophasic mixtures of aqueous enzyme solutions and varying fractions of fully miscible organic solvents,4, 5 pure hydrophilic or hydrophobic organic solvents that contain dispersed dried enzyme,1, 6 and biphasic aqueous–organic media with the enzyme dissolved in an aqueous solution and reactants that partition between the phases 7. All systems have their advantages and disadvantages,8, 9 and case studies of successful synthetic applications have been reported for each of them 7.…”

Section: Introductionmentioning

confidence: 99%

Optimized Biocatalytically Active Static Emulsions for Organic Synthesis in Nonaqueous Media

Kraume

Ansorge‐Schumacher

2011

ChemCatChem

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Literature reports biocatalytically active static emulsions (BASE) as promising systems for the preparation of biocatalysts designed for synthetic use in organic media. Their excellent catalytic performance is attributed to the numerous micropools of dissolved enzymes independently dispersed in silicone beads. Here, a systematic study of the structure and morphology of BASE and optimization in terms of bead size distribution and overall catalytic performance is presented. The study relies on beads obtained by using a novel preparation method that enables a considerably improved reproducibility of the particle size and catalytic activity in separate batches. A large interfacial area of 0.023 m2 g−1 material was calculated. The adjustment of bead composition increased the apparent catalytic activity of entrapped lipase A from Candida antarctica (CalA) to 0.71 U gBASE−1, which is almost twofold higher than that previously reported. The specific activity remained in the range of prototype BASE (0.21 U mgprotein−1), which nevertheless is about 53 times higher than that reported for CalA entrapped in a sol–gel.

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Systematic assessment of the stability of benzaldehyde lyase in aqueous–organic biphasic systems and its stabilization by modification with methoxy-poly(ethylene) glycol

Cited by 22 publications

References 42 publications

Nanoparticle Cages for Enzyme Catalysis in Organic Media

Nanoparticle Cages for Enzyme Catalysis in Organic Media

Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions

Optimized Biocatalytically Active Static Emulsions for Organic Synthesis in Nonaqueous Media

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