Systematic bio-fabrication of aptamers and their applications in engineering biology

Cai, Rongfeng; Chen, Xin; Zhang, Yuting; Wang, Xiaoli; Zhou, Nandi

doi:10.1007/s43393-022-00140-5

Cited by 14 publications

(5 citation statements)

References 159 publications

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“…Random substitution of ten nucleotides in MSA52 native sequence decreases the stem/loop ratio from 1.26 to 0.68 in the T-scrambled aptamer. It is known that the presence of secondary structures, such as hairpin loops, increases the bonding strengths of aptamers [ 31 ], and is important to the aptamer-target interactions [ 32 ]. In addition, GC content decreased from 54% to 48% when we modified the aptamer native sequence.…”

Section: Resultsmentioning

confidence: 99%

A capacitive laser-induced graphene based aptasensor for SARS-CoV-2 detection in human saliva

Moreira,

Qian,

Datta

et al. 2023

PLoS ONE

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SARS-CoV-2 virus induced CoVID-19 pandemic has accelerated the development of diagnostic tools. Devices integrated with electrochemical biosensors may be an interesting alternative to respond to the high demand for testing, particularly in contexts where access to standard detection technologies is lacking. Aptamers as recognition elements are useful due to their stability, specificity, and sensitivity to binding target molecules. We have developed a non-invasive electrochemical aptamer-based biosensor targeting SARS-CoV-2 in human saliva. The aptamer is expected to detect the Spike protein of SARS-CoV-2 wildtype and its variants. Laser-induced graphene (LIG) electrodes coated with platinum nanoparticles were biofunctionalized with a biotin-tagged aptamer. Electrochemical Impedance Spectroscopy (EIS) for BA.1 sensing was conducted in sodium chloride/sodium bicarbonate solution supplemented with pooled saliva. To estimate sensing performance, the aptasensor was tested with contrived samples of UV-attenuated virions from 10 to 10,000 copies/ml. Selectivity was assessed by exposing the aptasensor to non-targeted viruses (hCoV-OC43, Influenza A, and RSV-A). EIS data outputs were further used to select a suitable response variable and cutoff frequency. Capacitance increases in response to the gradual loading of the attenuated BA.1. The aptasensor was sensitive and specific for BA.1 at a lower viral load (10–100 copies/ml) and was capable of discriminating between negative and positive contrived samples (with strain specificity against other viruses: OC43, Influenza A, and RSV-A). The aptasensor detected SARS-CoV-2 with an estimated LOD of 1790 copies/ml in contrived samples. In human clinical samples, the aptasensor presents an accuracy of 72%, with 75% of positive percent of agreement and 67% of negative percent of agreement. Our results show that the aptasensor is a promising candidate to detect SARS-CoV-2 during early stages of infection when virion concentrations are low, which may be useful for preventing the asymptomatic spread of CoVID-19.

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Section: Resultsmentioning

confidence: 99%

A capacitive laser-induced graphene based aptasensor for SARS-CoV-2 detection in human saliva

Moreira,

Qian,

Datta

et al. 2023

PLoS ONE

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“…specific, Safe, and efficient delivery of siRNAs into special cells is of high importance in the therapy field. [15] Nanobodies Camels and sharks have antibodies that do not have a light chain and only contain a heavy chain, and they are called HCAbs (Heavy Chain Antibodies). These antibodies do not have CH1 domains, but they have CH3 and CH2 domains, which are very similar to conventional antibodies.…”

Section: Aptamersmentioning

confidence: 99%

A Review of the Effectiveness of Using Nanoparticles in the Treatment and Diagnosis of Breast Cancer

Kohli,

Mangla,

Haque

et al. 2024

Clin Cancer Investig J

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Cancer has been one of the main concerns of the health sector in recent years. The utilization of nanoparticles in medical science has created new possibilities for diagnosing, imaging tumors, and treating cancer in humans. This article reviews the utilization of inorganic and organic nanoparticles in cancer treatment and diagnosis. The method of gathering and summarizing the information was through the research conducted in connection with the structure of various types of nanoparticles and their application in imaging, diagnosis, and drug delivery systems in cancer treatment. From inorganic nanoparticles including quantum particles, which have a central core with magnetic attributes, for cancer imaging and detection, as well as from organic nanoparticle systems including dendrimers, aptamers, solid lipid nanoparticles, liposomes, and nanoparticles in transportation. Various drugs and ligands can be used. Aptamers are oligonucleotide or peptide molecules that bind to protein or target cells and are used in diagnosis, treatment, drug delivery, and molecular imaging. Also, nanobodies are antibody molecules that are widely utilized in cancer treatment and diagnosis. According to the obtained results, there are still limitations and challenges to the utilization of nanoparticles in medicine, however, it is vision that shortly nanoparticles will create a great revolution not only in oncology but also in medicine.

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“…Aptamers can also be readily chemically modified in order to increase the range of their targets, increase their binding affinity and conjugation efficiency to their targets or nanomaterials, and promote their stability. These strategies range from adding an active functionality at either the 3′ or 5′ terminals to several more complex chemical conjugation methods, including thiol-maleimide, carbodiimide, oxidative, thiol–gold coordination, avidin–biotin coupling, and click chemistry [ 1 , 40 ].…”

Section: Aptamers and Aptasensorsmentioning

confidence: 99%

Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications

Economou,

Kokkinos,

Bousiakou

et al. 2023

Sensors

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Aptamers are short oligonucleotides designed to possess high binding affinity towards specific target compounds (ions, molecules, or cells). Due to their function and unique advantages, aptamers are considered viable alternatives to antibodies as biorecognition elements in bioassays and biosensors. On the other hand, paper-based devices (PADs) have emerged as a promising and powerful technology for the fabrication of low-cost analytical tools, mainly intended for on-site and point-of-care applications. The present work aims to provide a comprehensive overview of paper-based aptasensors. The review describes the fabrication methods and working principles of paper-based devices, the properties of aptamers as bioreceptors, the different modes of detection used in conjunction with aptasensing PADs, and representative applications for the detection of ions, small molecules, proteins, and cells. The future challenges and prospects of these devices are also discussed.

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Systematic bio-fabrication of aptamers and their applications in engineering biology

Cited by 14 publications

References 159 publications

A capacitive laser-induced graphene based aptasensor for SARS-CoV-2 detection in human saliva

A capacitive laser-induced graphene based aptasensor for SARS-CoV-2 detection in human saliva

A Review of the Effectiveness of Using Nanoparticles in the Treatment and Diagnosis of Breast Cancer

Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications

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