Systematic evaluation of ICG and trypan blue related effects on ARPE-19 cells in vitro

Peters, Swaantje; Altvater, Andreas; Bopp, Silvia; Vonthein, Reinhard; Szurman, Peter; Spitzer, Martin S.; Warga, M.; Lueke, Matthias; Bartz-Schmidt, Karl-Ulrich; Grisanti, Salvatore

doi:10.1016/j.exer.2007.08.024

Cited by 25 publications

(23 citation statements)

References 35 publications

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“…This assumption was supported by Ueno et al who demonstrated that subretinal injection of 0.25 mg/ml BBG induced no detectable toxicity in opposite to the other tested dyes like ICG and TB [26]. However, in a recently published in-vitro study testing the ICG-related cytotoxicity on human RPE cells it was found that concentrations lower than 1 mg/ml and incubation times shorter than 5 min as used in clinical practice were well tolerated [27]. The hypothesis of a time-and dosedependent toxic effect of ICG was confirmed by a recent published review of the literature [28].…”

Section: Discussionmentioning

confidence: 78%

Electrophysiological effects of Brilliant Blue G in the model of the isolated perfused vertebrate retina

Lüke

Januschowski

Beutel

et al. 2008

Graefes Arch Clin Exp Ophthalmol

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Section: Discussionmentioning

confidence: 78%

Electrophysiological effects of Brilliant Blue G in the model of the isolated perfused vertebrate retina

Lüke

Januschowski

Beutel

et al. 2008

Graefes Arch Clin Exp Ophthalmol

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“…Shortly after ICG had been recommended for ILM peeling,3 a less favourable functional outcome and a high incidence of peripheral visual field defects6 15 16 led to a still ongoing discussion on potential toxic effects of ICG which may be related to its photosensitising properties 17 18. The safety margin of ICG is unclear, and some authors did not observe any dye-related complications 19 20.…”

Section: Discussionmentioning

confidence: 99%

An in vivo evaluation of Brilliant Blue G in animals and humans

Remy

Thaler

Schumann

et al. 2008

British Journal of Ophthalmology

134

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Background/Aims: To evaluate the retinal toxicity of Brilliant Blue G (BBG) following intravitreal injection in rat eyes and examine the biocompatibility and the staining properties in humans. Methods: BBG was injected into the 11 rat eyes to evaluate toxic effects with balanced salt solution (BSS) serving as control. Retinal toxicity was assessed by retinal ganglion cell (RGC) counts and by light microscopy 7 days later. In addition, BBG was applied during vitrectomy for macular hole (MH) (n = 15) or epiretinal membranes (ERM) (n = 3) in a prospective, non-comparative consecutive series of patients. Before and after surgery, all patients underwent a complete clinical examination including measurement of best corrected visual acuity (VA) and intraocular pressure, perimetry, fundus photography and optical coherence tomography. Patients were seen 1 day before surgery and then in approximately four weeks intervals.

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“…Compared to other cell culture models, such as the human RPE cell line ARPE-19 [7,8,9,10], retinal ganglion cells [11] and human primary RPE cells [12,13], the HFRPE culture model shows a robust cell culture to exhibit the morphology and physiology of healthy RPE in vivo [14,15]. It is a useful model to test the effect of novel vital dyes on outer BRB function before clinical application.…”

Section: Introductionmentioning

confidence: 99%

Effect of Novel Vital Dyes on Outer Blood-Retina Barrier Function in Cultured Human Retinal Pigment Epithelium

Liu

Meyer

Stanzel³

2013

Ophthalmologica

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Purpose: To assess tight junction (TJ) integrity in cultured human fetal retinal pigment epithelium (HFRPE) after exposure to clinically relevant novel vital dyes. Methods: HFRPE floater cells were harvested from RPE primary cultures of 4 donor eyes and seeded on polyester Transwell® for 4-6 weeks. The apical compartments of well-differentiated cultures were exposed to 0.005 mg/ml Coomassie violet R200 (CVR), methyl 2B (M2B) or Orange II. Periods of 30-300 s were chosen to mimic surgical exposure times, while 3 h was used for toxicity assays, with subsequent washout. Cell-cell junctions were studied by immunofluorescence (zonula occludens-1, ZO-1). Transepithelial electrical resistance (TER) was measured regarding blood-retina barrier (BRB) function. Results: At 4-6 weeks after confluence, HFRPE had grown into pigmented hexagonal monolayers with stable TER values (451-1,520 Ω·cm²). After 300-second dye treatments, a continuous ZO-1 signal was detected in all vital dye-treated groups 1.5 h after exposure, whereas trypsin controls showed patchy loss of the TJ stain. TER of CVR-, M2B- and Orange-II-treated groups had dropped 1.5 h after exposure to 148 ± 58.4, 162 ± 23.7 and 164 ± 18.5 Ω·cm², respectively, compared to 73 ± 44.9 Ω·cm² in positive controls. After 3 h of exposure to 0.005 mg/ml vital dyes in thick drops, TER maintained similar levels to those prior to exposure (90.8 ± 4.7% of the original values, 93.8 ± 6.5 and 91.9 ± 3.6%, respectively), together with no difference from the vehicle controls (94.8 ± 6.6%). TER values recovered in all groups to prior levels within 3 days. Conclusion: Novel vital dyes (CVR, M2B and Orange II) caused no outer BRB function alteration.

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Systematic evaluation of ICG and trypan blue related effects on ARPE-19 cells in vitro

Cited by 25 publications

References 35 publications

Electrophysiological effects of Brilliant Blue G in the model of the isolated perfused vertebrate retina

Electrophysiological effects of Brilliant Blue G in the model of the isolated perfused vertebrate retina

An in vivo evaluation of Brilliant Blue G in animals and humans

Effect of Novel Vital Dyes on Outer Blood-Retina Barrier Function in Cultured Human Retinal Pigment Epithelium

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