Systematic identification of TPS genes in Gossypium and their characteristics in response to flooding stress

Abstract

Terpene synthases (TPS) is a key enzyme in the synthesis of plant terpenoids. Studies on TPSs have not been reported in Gossypium barbadense and Gossypium arboreum. 260 TPSs were identified in Gossypium, including 71 in Gossypium hirsutum, 75 in Gossypium. barbadense, 60 in Gossypium. arboreum, and 54 in Gossypium raimondii. We systematically analyzed the TPS gene family of Gossypium from three aspects: gene structure, evolutionary process and gene function. (1) Gene structure: Based on the protein structure of two conserved domains (PF01397 and PF03936), the TPS gene family is divided into five clades: TPS -a, -b, -c, -e/f and -g. (2) Evolution: Whole genome duplication and segmental duplication are the main modes of TPS gene amplification. (3) Function: The abundance of cis-acting elements may reveal the functional diversity of TPSs in cotton. TPS gene has tissue specific expression in cotton. The hypomethylation of the exon of TPSs may help to enhance the adaptability of cotton to flooding stress. In conclusion, this study can broaden the understanding of structure-evolution-function of the TPS gene family, and provide reference for the mining and verification of new genes.

Genome-wide identification of terpenoid synthase family genes in Gossypium hirsutum and functional dissection of its subfamily cadinene synthase A in gossypol synthesis

“…To reveal whether replication and loss have occurred, we performed a colinear analysis of all TPS genes in diploid G. arboreum and G. raimondii and tetraploid G. hirsutum ( Figure 3 ). When PF01397 and PF03936 were used as indexes to scan the whole genome for identification of TPS family genes, it was found that there were 75 TPS genes in G. arboreum , 60 in G. raimondii , and only 91 in G. hirsutum without any filtration ( Huang et al., 2018 ; Cui et al., 2023 ). Collinearity analysis showed that 22 sequences showed collinearity between G. arboreum and G. hirsutum , and 20 sequences showed collinearity between G. raimondii and G. hirsutum .…”

Time-Series Gene Expression Analysis of Terpene Synthase Gene Family Reveals Its Evolutionary Dynamics in a Tobacco Plant

“…We found this pattern concurs with the overall molecular phylogeny of Solanaceae, where the genus Nicotiana is at a more basal level than Capsicum and Solanum on the phylogenetic tree 22 . It is observed that tandem duplication played an important role in the occurrence of tabacumexclusive clades 27 . The tandem duplication accounts for the formation of gene clusters of the same gene family and confers new phenotypic traits 28 .…”