This study aims to analyze karyotypes of Sulawesi’s small mammals with Giemsa banding technique. Five fruit bats species (Boneia bidens, Dobsonia viridis, Styloctenium wallacei, Thoopterus nigrescens, Thoopterus suhaniahae) and eight rat species (Bunomys andrewsi, Bunomys chrysocomus, Bunomys heinrichi, Paruromys sp., Rattus dommermani, Rattus exulans, Rattus hoffmanni, Taeromys celebensis) from Mt. Bawakaraeng, South Sulawesi were analyzed. Karyotypes of three species in this study were described for the first time. There were two species of bats and one species of rats showed the best of karyotypes. B. bidens has 2n = 30, FN = 53, FNa = 50, sub metacentric and telocentric for X and Y chromosomes respectively. T. suhaniahae has 2n = 38, FN = 64, FNa = 60 and metacentric X chromosomes. R. hoffmanni has 2n = 44, FN = 61, FNa = 59 and telosentric X chromosomes.