Systematic Position and Phylogeny

Ley, Paul De; Blaxter, Mark

doi:10.1201/b12614-2

Cited by 213 publications

(184 citation statements)

References 29 publications

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“…The ovaries of free-living females of the nematode superfamily Strongyloidoidea (De Ley and Blaxter, 2001;Dorris, et al, 2002) are very similar in structure to the gonads of C. elegans hermaphrodites. This has made it possible to directly apply standard methods for gonadal microinjection of C. elegans (Evans 2006) to Strongyloides spp.…”

Section: Introductionmentioning

confidence: 83%

Strongyloides stercoralis: Cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3′ UTR

Junio

Massey

et al. 2008

Experimental Parasitology

116

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Transgenesis is a valuable methodology for studying gene expression patterns and gene function. It has recently become available for research on some parasitic nematodes, including Strongyloides stercoralis. Previously, we described a vector construct, comprising the promoter and 3′ UTR of the S. stercoralis gene Ss era-1 that gives expression of GFP in intestinal cells of developing F1 progeny. In the present study, we identified three new S. stercoralis promoters, which, in combination with the Ss era-1 3′ UTR, can drive expression of GFP or the red fluorescent protein, mRFPmars, in tissuespecific fashion. These include Ss act-2, which drives expression in body wall muscle cells, Ss gpa-3, which drives expression in amphidial and phasmidial neurons and Ss rps-21, which drives ubiquitous expression in F1 transformants and in the gonads of microinjected P0 female worms. Concomitant microinjection of vectors containing GFP and mRFPmars gave dually transformed F1 progeny, suggesting that these constructs could be used as co-injection markers for other transgenes of interest. We have developed a vector "toolkit" for S. stercoralis including constructs with the Ss era-1 3′ UTR and each of the promoters described above.

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Section: Introductionmentioning

confidence: 83%

Strongyloides stercoralis: Cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3′ UTR

Junio

Massey

et al. 2008

Experimental Parasitology

116

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“…An interesting observation in our study was the presence of 5-HT-IR structures located along the whole body of T. pseudospiralis. Nematodes of the order Trichinellida (Ley & Blaxter, 2002) are characterized by having bacillary bands (rows of hypodermal gland cells) present also in Dioctophymatida (e.g. Eustrongylides).…”

Section: Discussionmentioning

confidence: 99%

Serotonin in Trichinella pseudospiralis: An immunocytochemical study

et al. 2016

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SummaryThis is the fi rst report on the presence and localization of the neurotransmitter serotonin (5-HT) in the nervous system of the nematode Trichinella pseudospiralis, the causative agent of trichinellosis. The orientation of the 5-HT-immunoreactive (5-HT-IR) nerve cells in the adult worm is described. In the anterior region of the worm 5-HT-IR occurs in 7 neurons. Longitudinal nerve cords extend posteriorly from the anterior end. They are connected by transverse commissures. The vulval area is intensively supplied with 5-HT-IR nerve cells and fi bres forming a plexus. Two rows of small 5HT-IR structures, hypodermal glands, are visible along the whole nematode body. Because of the conserved structural features among nematodes the 5-HT-IR neurons observed are likely to have counterparts in the model worm, Caenorhabditis elegans. Some basic differences are evident and demand further study.

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“…Next, the suspension containing the nematodes was clarified by sugar centrifugal floatation (JENKINS, 1964) and kept in formalin (4% formaldehyde). The total number of nematodes was estimated on a counting slide, and 200 random individuals were identified to genus level under an optical microscope, in accordance with Goseco et al (1974aGoseco et al ( , 1974b, Bongers (1987), Fortuner et al (1988) and De Ley and Blaxter (2002).…”

Section: Sampling Extraction and Identification Of Nematodesmentioning

confidence: 99%

Depth-stratified soil sampling for assessing nematode communities

et al. 2016

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This study assessed the importance of stratified soil sampling on the detection (and therefore the distribution) of nematode communities and the differentiation of ecosystems by collecting stratified soil samples at intervals of 10 cm and non-stratified samples from 0 to 30 cm in two soil management systems (no-tillage and conventional tillage) and in a native forest fragment. The nematode frequency and prominence values were obtained after extraction by successive screening operations, sugar floatation clarification and the identification of nematodes to the genus level. The nematode communities were compared two-by-two based on Sorensen's community coefficient (CC) and the percentage similarity (PS). Relative abundances of functional guilds were subjected to a principal component analysis (PCA) and classified in dendrograms. Thirty-two edaphic nematode genera were found, and the nematode communities sampled on a non-stratified basis in the soil profile exhibited a high level of similarity because they could not be accurately characterized. Genera with low abundances were not detected. In the stratified samples, we were able to classify and group the nematodes present at different depths, mainly from 0 to 10 cm. Stratified soil sampling allowed a more accurate characterization and greater differentiation of nematode communities, identifying taxa that occurred at lower abundance levels, irrespective of frequency. Key words: Bioindicators. Free-living nematodes. Nematofauna. Similarity. Vertical distribution. ResumoCom o objetivo de avaliar a importância da amostragem estratificada de solos na detecção (assim como na distribuição) de comunidades de nematoides e na diferenciação de ecossistemas, amostras de solo estratificadas em intervalos de 10 cm e não estratificadas de 0 a 30 cm foram coletadas em dois sistemas de prepare do solo (plantio direto e preparo convencional), e em um fragmento de floresta nativa. Após extração por peneiramento sucessivo, clarificação por flotação em sacarose e identificação dos nematoides até nível de gênero, a frequência e proeminência dos nematoides foram obtidas. As comunidades de nematoides foram comparadas duas a duas, baseadas no Coeficiente de comunidade de Sorensen e na Similaridade percentual (PS). Abundâncias relativas das guildas funcionais foram submetidas à Análise de componentes principais (PCA) e classificadas em dendrogramas. Trinta e dois gêneros de nematoides edáficos foram encontrados e as comunidades de nematoides amostradas de forma não estratificada no perfil do solo apresentaram um maior nível de similaridade entre si, pois não foram corretamente caracterizadas e os gêneros com baixa abundância não foram detectados. Nas amostras estratificadas, foi possível classificar e agrupar os nematoides presentes em diferentes profundidades, principalmente

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Systematic Position and Phylogeny

Cited by 213 publications

References 29 publications

Strongyloides stercoralis: Cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3′ UTR

Strongyloides stercoralis: Cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3′ UTR

Serotonin in Trichinella pseudospiralis: An immunocytochemical study

Depth-stratified soil sampling for assessing nematode communities

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